Ginsenoside as a new stabilizer enhances the transfection efficiency and biocompatibility of cationic liposome

Abstract

Ginsenoside-based compounds were utilized as membrane stabilizers to prepare CLs (GCLs). GCLs are demonstrated as promising non-viral vectors with excellent transfection efficiency and biocompatibility, possessing great potential for gene delivery.

Extracellular Vesicles as Novel Drug-Delivery Systems through Intracellular Communications

“…Therefore, they have attracted attention as a next-generation modality that sets them apart from other pharmaceutical products. However, nucleic-acid drugs have problems such as low intracellular-delivery efficiency due to low cell-membrane permeability, their large molecular weight, and elimination by the cellular immune system [93][94][95][96][97]. To solve these problems, the development of a DDS using the chemical modification of nucleic acids and liquid nanoparticles is underway.…”

A lipid–polymer hybrid nanoparticle (LPN)-loaded dissolving microneedle patch for promoting hair regrowth by transdermal miR-218 delivery

“…15,16 In the LPN formulation, cholesterol and natural saturated phospholipid lecithin were used to stabilize the structure of LPNs, as in cellular membranes; 17 the outer DSPE-PEG (distearoyl phosphoethanolamine-polyethylene glycol) layer was used as a stealth coating to prolong the in vivo circulation time; 18 the cationic polymer polyvinylamine (PVAm) was utilized as a core to facilitate endosomal escape in which the therapeutic miRNAs were encapsulated. [19][20][21] Furthermore, the stratum corneum is another vital hurdle of transdermal RNA therapy. A microneedle (MN) patch, a promising transdermal device, was flexibly adjusted to fit irregular skin surfaces and to achieve different depths of delivery.…”

Gasdermin E plasmid DNA/indocyanine green coloaded hybrid nanoparticles with spatiotemporal controllability to induce pyroptosis for colon cancer treatment

“…31 Cationic liposome is an ideal nonviral vector; however, the cytotoxicity and low efficiency in vivo always restrict its application. 32,33 Inclusion of hydrophobic poly(lactide-co-glycolide) (PLGA) inner core inside of the lipid membrane can contribute to pack water-insoluble drug and avoid drug leakage. 22,34 And PEGylation of the nanoparticle with PEG-containing lipid membrane can prevent serum protein binding, prolong circulation time in vivo and shield the toxicity of cationic lipids to some extent.…”

“…Nonviral vectors with easy preparation process, low cost, and good compatibility can avoid the potential immunogenicity and carcinogenicity of viral vectors 31 . Cationic liposome is an ideal nonviral vector; however, the cytotoxicity and low efficiency in vivo always restrict its application 32,33 . Inclusion of hydrophobic poly(lactide‐co‐glycolide) (PLGA) inner core inside of the lipid membrane can contribute to pack water‐insoluble drug and avoid drug leakage 22,34 .…”