Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

Kochan, Ewa; Caban, Sylwia; Szymańska, Grażyna; Szymczyk, Piotr; Lipert, Anna; Kwiatkowski, Paweł; Sienkiewicz, Monika

doi:10.17951/c.2017.72.1.15-26

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…There are various kinds of bioreactors. Stirred-tank bioreactors are the most commonly used (they were utilized by Wang et al [44] and Kochan et al [51]), since they allow easier accumulation of cells at various stages due to their large capacities and for their capability to scale up nutrients [52]. The airlift and balloon-type airlift bioreactors are also utilized for ginsenoside production.…”

Section: Bioreactor: Large-scale Propagationmentioning

confidence: 99%

Biotechnological Interventions for Ginsenosides Production

2020

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Ginsenosides are secondary metabolites that belong to the triterpenoid or saponin group. These occupy a unique place in the pharmaceutical sector, associated with the manufacturing of medicines and dietary supplements. These valuable secondary metabolites are predominantly used for the treatment of nervous and cardiac ailments. The conventional approaches for ginsenoside extraction are time-consuming and not feasible, and thus it has paved the way for the development of various biotechnological approaches, which would ameliorate the production and extraction process. This review delineates the biotechnological tools, such as conventional tissue culture, cell suspension culture, protoplast culture, polyploidy, in vitro mutagenesis, hairy root culture, that have been largely implemented for the enhanced production of ginsenosides. The use of bioreactors to scale up ginsenoside yield is also presented. The main aim of this review is to address the unexplored aspects and limitations of these biotechnological tools, so that a platform for the utilization of novel approaches can be established to further increase the production of ginsenosides in the near future.

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Section: Bioreactor: Large-scale Propagationmentioning

confidence: 99%

Biotechnological Interventions for Ginsenosides Production

2020

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“…After a 25-day growth cycle at a temperature of 24 � 1°C and pH of 6.0, the cell growth and ginsenoside content reached 23.65 g/L and 677.25 mg/L, respectively. Kochan et al [81] cultured the callus cells derived from ginseng leaves in the liquid medium containing 1 mg/L 2,4-D and 0.1 mg/L kinetin at a pH of 5.6 -5.8, temperature of 26°C, and humidity of 90 %. The suspension culture was maintained in the dark for 40 days.…”

Section: Cell Suspension Culture Of American Ginsengmentioning

confidence: 99%

Recent Advances in the Tissue Culture of American Ginseng (Panax quinquefolius)

Qiang

Jin

Phillips

et al. 2020

Chemistry & Biodiversity

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The in vitro tissue culture of medicinal plants is considered as a potential source for plant-derived bioactive secondary metabolites. The in vitro tissue culture of American ginseng has wide commercial applications in pharmaceutical, nutraceutical, food, and cosmetic fields with regard to the production of bioactive compounds such as ginsenosides and polysaccharides. This review highlights the recent progress made on different types of tissue culture practices with American ginseng, including callus culture, somatic embryo culture, cell suspension culture, hairy root culture, and adventitious root culture. The tissue culture conditions for inducing ginseng callus, somatic embryos, cell suspension, hairy roots, and adventitious roots were analyzed. In addition, the optimized conditions for increasing the production of ginsenosides and polysaccharides were discussed. This review provides references for the use of modern biotechnology to improve the production of bioactive compounds from American ginseng, as well as references for the development and sustainable utilization of American ginseng resources.

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How Do Extraction Methods and Biotechnology Influence Our Understanding and Usages of Ginsenosides?: A Critical View and Perspectives

Hano¹,

Drouet²,

Addi³

et al. 2022

Ginseng - Modern Aspects of the Famed Traditional Medicine

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Ginseng saponins, aka ginsenosides, are bioactive phytochemicals from Panax species. Panax comes from the Greek word “panakos,” which means “cure-all.” Owing to their involvement in the creation of numerous medications and nutritional supplements, ginseng saponins play an essential part, especially in the pharmaceutical sector. The main ginsenosides (i.e., Rb1, Rb2, Rc, Rd and Rf) are extracted using a variety of extraction methods, although from a limited number of Panax species. However, more than ca 1000 unique ginsenosides and 18 Panax species have been reported so far, thus demonstrating our present challenge in better understanding of the potential medicinal uses of these compounds. Moreover, ginsenoside production and extraction methods are both time-consuming and inefficient, which has stimulated the development of several efficient extraction and biotechnological technologies to speed up these processes. In this chapter, we highlighted the need to expand the cutting-edge research approaches involving these unique ginsenosides to better understand their biological activities and discover new bioactive ginsenosides as well. The main objective of this chapter is to discuss the undiscovered aspects and limitations of the current biotechnological and extraction technologies, eventually to provide a platform for the production of these unique ginsenosides.

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Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

Cited by 3 publications

References 24 publications

Biotechnological Interventions for Ginsenosides Production

Biotechnological Interventions for Ginsenosides Production

Recent Advances in the Tissue Culture of American Ginseng (Panax quinquefolius)

How Do Extraction Methods and Biotechnology Influence Our Understanding and Usages of Ginsenosides?: A Critical View and Perspectives

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