Ginsenoside Rb1 Ameliorates Age-Related Myocardial Dysfunction by Regulating the NF-κB Signaling Pathway

Abstract

Age-related myocardial dysfunction is a very large healthcare burden. Here, we aimed to investigate whether ginsenoside Rb1 (Rb1) improves age-related myocardial dysfunction and to identify the relevant molecular mechanism. Young mice and aged mice were injected with Rb1 or vehicle for 3 months. Then, their cardiac function was inspected by transthoracic echocardiography. Serum and myocardium tissue were collected from all mice for histological or molecular expression analyses, including aging-related proteins, markers relevant to fibrosis and inflammation, and markers indicating the activation of the nuclear factor-kappa B (NF-[Formula: see text]B) pathway. Compared with the control condition, Rb1 treatment significantly increased the ejection fraction percentage and significantly decreased the internal diameter and volume of the left ventricle at the end-systolic and end-diastolic phases in aged mice. Rb1 treatment reduced collagen deposition and collagen I, collagen III, and transforming growth factor-[Formula: see text]1 protein expression levels in aged hearts. Rb1 also decreased the aging-induced myocardial inflammatory response, as measured by serum or myocardial interleukin-6 and tumor necrosis factor-[Formula: see text] levels. Furthermore, Rb1 treatment in aged mice increased cytoplasmic NF-[Formula: see text]B but decreased nuclear NF-[Formula: see text]B, which indicated the suppression of the NF-[Formula: see text]B signaling pathway by regulating the translocation of NF-[Formula: see text]B. Rb1 could alleviate aging-related myocardial dysfunction by suppressing fibrosis and inflammation, which is potentially associated with regulation of the NF-[Formula: see text]B signaling pathway.

Kirenol Inhibits B[a]P‐Induced Oxidative Stress and Apoptosis in Endothelial Cells via Modulation of the Nrf2 Signaling Pathway

“…Another observation of that study was that this phenomenon is linked to abnormal stress (mediated by ROS) and inflammation [44]. In addition to getting the Bax/Bcl-2 ratio's dysregulation attenuated, the extracts of KRL are also likely to prevent myocardial cells' apoptotic death among animals that have reached an advanced age [45]. KRL's cytoprotective impact is linked to enhanced activity of antioxidants and lower oxidative stress.…”

The anti-aging mechanism of ginsenosides with medicine and food homology

“…Ke et al found that Rb1 could significantly improve myocardial dysfunction and reduce the left ventricular internal diameter and volume in aged mice. 52 Further studies showed that Rb1 could reduce collagen deposition and the protein expression levels of collagen I, collagen III and TGF-β1 in the aging heart. In addition, NF-κB was shown to undergo significant nuclear translocation in the presence of Rb1.…”

Effects of Shenmai injection against chronic heart failure: a meta-analysis and systematic review of preclinical and clinical studies

“…GRg1 can suppress TNF-α mediated NF-κB activation, thereby attenuating cardiac hypertrophy induced by abdominal aortic constriction (Tang et al, 2016). On the other hand, GRb1 can improve energy metabolism in cardiac myocytes (Li et al, 2023b), inhibit inflammation (Ke et al, 2020;Wang et al, FIGURE 12 (Continued).…”

“…Frontiers in Pharmacology frontiersin.org Funnel plot of the LVEF. Frontiers in Pharmacology frontiersin.org 2021), suppress myocardial hypertrophy (Wang et al, 2021), and inhibit myocardial fibrosis (Ke et al, 2020). Relevant studies suggest that GRb1 improves energy metabolism in cardiac myocytes by activating the PPARα pathway, possibly through the inhibition of Fas-associated death domain (Li et al, 2023b).…”

“…Relevant studies suggest that GRb1 improves energy metabolism in cardiac myocytes by activating the PPARα pathway, possibly through the inhibition of Fas-associated death domain (Li et al, 2023b). GRb1 may alleviate age-related cardiac dysfunction by inhibiting fibrosis and inflammation, which may be related to the regulation of the NF-κB pathway (Ke et al, 2020). Ophiopogon contains effective chemical components such as steroidal saponins, high isoflavones and carbohydrates (Chen et al, 2016).…”