Ginsenoside Rg1 attenuates high glucose‑induced endothelial barrier dysfunction in human umbilical vein endothelial cells by protecting the endothelial glycocalyx

Zhu, Tingting; Wang, Honglian; Wang, Li; Zhong, Xia; Huang, Wei; Deng, Xian; Guo, Hengli; Xiong, Jianfeng; Xu, Youhua; Fan, Junming

doi:10.3892/etm.2019.7378

Cited by 11 publications

(9 citation statements)

References 41 publications

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“…Endothelial glycocalyx maintains the vascular function and plays an important role in vascular permeability, leucocyte, platelet adhesion as well as transduction of fluid shearing forces ( 23 , 24 ). FITC-WGA has been shown to bind to the glycoprotein of the glycocalyx ( 25 ).…”

Section: Discussionmentioning

confidence: 99%

Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells

et al. 2021

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Filtration barrier injury induced by high glucose (HG) levels leads to the development of diabetic nephropathy. The endothelial glycocalyx plays a critical role in glomerular barrier function. In the present study, the effects of piperazine ferulate (PF) on HG-induced filtration barrier injury of glomerular endothelial cells (GEnCs) were investigated and the underlying mechanism was assessed. Immunofluorescence was used to observe the distribution of the glycocalyx as well as the expression levels of syndecan-1 and Zonula occludens-1 (ZO-1). Endothelial permeability assays were performed to assess the effects of PF on the integrity of the filtration barrier. Protein and mRNA expression levels were measured by western blotting and reverse transcription-quantitative PCR analyses, respectively. In vitro experiments revealed that adenosine monophosphate-activated protein kinase (AMPK) mediated HG-induced glycocalyx degradation and endothelial barrier injury. PF inhibited the HG-induced endothelial barrier injury and restored the expression levels of heparanase-1 (Hpa-1), ZO-1 and occludin-1 by AMPK. In vivo assays demonstrated that PF reduced the expression levels of Hpa-1, increased the expression levels of ZO-1 and attenuated glycocalyx degradation in the glomerulus. These data suggested that PF attenuated HG-induced filtration barrier injury of GEnC by regulating AMPK expression.

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Section: Discussionmentioning

confidence: 99%

Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells

et al. 2021

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“…Rg 1 is the main active ingredient extracted from Panax notoginseng and has been widely used to prevent vascular damage. Recent research ( Zhu et al, 2019 ) showed that high glucoscouldan induce endothelial glycocalyx disorders and increase the expression of heparanase mRNA in HUVEC, and Rg1 treatment can reverse this expression. In addition, after high glucose stimulation, Rg1 treatment can reduce transendothelial resistance and transendothelial albumin pass rate.…”

Section: Protective Effect Of Panax Ginseng Camey On Renal Innate Cellsmentioning

confidence: 99%

Panax Ginseng C.A.Mey. as Medicine: The Potential Use of Panax Ginseng C.A.Mey. as a Remedy for Kidney Protection from a Pharmacological Perspective

Jin

Zhang

et al. 2021

Front. Pharmacol.

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Panax ginseng C.A.Mey. has been widely consumed as food/diet supplements from natural sources, and its therapeutic properties have also aroused widespread concern. Therapeutic properties of Panax ginseng C.A.Mey. such as anti-inflammatory, ameliorating chronic inflammation, enhancing the immunity, resisting the oxidation again, and regulating the glucose and lipid metabolism have been widely reported. Recent years, lots of interesting studies have reported the potential use of Panax ginseng C.A.Mey. in the management of DKD. DKD has become the leading cause of end-stage renal disease worldwide, which increases the risk of premature death and poses a serious financial burden. Although DKD is somehow controllable with different drugs such as Angiotensin-Converting Enzyme Inhibitors (ACEI), Angiotensin Receptor Blockers (ARB) and lowering-glucose agents, modern dietary changes associated with DKD have facilitated research to assess the preventive and therapeutic merits of diet supplements from natural sources as medicine including Panax ginseng C.A.Mey. Findings from many scientific evidences have suggested that Panax ginseng C.A.Mey. can relieve the pathological status in cellular and animal models of DKD. Moreover, a few studies showed that alleviation of clinical phenotype such as reducing albuminuria, serum creatinine and renal anemia in DKD patients after application or consumption of Panax ginseng C.A.Mey.. Therefore, this review aims to discuss the effectiveness of Panax ginseng C.A.Mey. as medicine for targeting pathological phenotypes in DKD from a pharmacological perspective. This review will provide new insights into the potential understanding use of Panax ginseng C.A.Mey. in the management of DKD in clinical settings.

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“…Several reports have highlighted the following functions of the GCX: mechanotransduction, 123 barrier protection 124 and regulation of permeability, and other functions. Regarding mechanotransduction, due to the transmembrane nature of GCX core proteins, Tarbell and colleagues have noted that the GCX transduces shear and stretch forces into biomolecular responses of ECs.…”

Section: Endothelial Glycocalyx: Its Functions and Implications For Gmentioning

confidence: 99%

Endothelial Glycocalyx-Mediated Intercellular Interactions: Mechanisms and Implications for Atherosclerosis and Cancer Metastasis

Mensah

Nersesyan

Ebong

2020

Cardiovasc Eng Tech

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Purpose The endothelial glycocalyx (GCX) plays a critical role in the health of the vascular system. Degradation of the GCX has been implicated in the onset of diseases like atherosclerosis and cancer because it disrupts endothelial cell (EC) function that is meant to protect from atherosclerosis and cancer. Examples of such EC function include interendothelial cell communication via gap junctions and receptor-mediated interactions between endothelial and tumor cells. This review focuses on GCX-dependent regulation of these intercellular interactions in healthy and diseased states. The ultimate goal is to build new knowledge that can be applied to developing GCX regeneration strategies that can control intercellular interaction in order to combat the progression of diseases such as atherosclerosis and cancer. Methods In vitro and in vivo studies were conducted to determine the baseline expression of GCX in physiologically relevant conditions. Chemical and mechanical GCX degradation approaches were employed to degrade the GCX. The impact of intact versus degraded GCX on intercellular interactions was assessed using cytochemistry, histochemistry, a Lucifer yellow dye transfer assay, and confocal, intravital, and scanning electron microscopy techniques. Results Relevant to atherosclerosis, we found that GCX stability determines the expression and functionality of Cx43 in gap junction-mediated EC-to-EC communication. Relevant to cancer metastasis, we found that destabilizing the GCX through either disturbed flow-induced or enzyme induced GCX degradation results in increased E-selectin receptor-mediated EC-tumor cell interactions. Conclusion Our findings lay a foundation for future endothelial GCX-targeted therapy, to control intercellular interactions and limit the progression of atherosclerosis and cancer.

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Ginsenoside Rg1 attenuates high glucose‑induced endothelial barrier dysfunction in human umbilical vein endothelial cells by protecting the endothelial glycocalyx

Cited by 11 publications

References 41 publications

Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells

Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells

Panax Ginseng C.A.Mey. as Medicine: The Potential Use of Panax Ginseng C.A.Mey. as a Remedy for Kidney Protection from a Pharmacological Perspective

Endothelial Glycocalyx-Mediated Intercellular Interactions: Mechanisms and Implications for Atherosclerosis and Cancer Metastasis

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