Piperazine ferulate prevents high‑glucose‑induced filtration barrier injury of glomerular endothelial cells

Abstract

Filtration barrier injury induced by high glucose (HG) levels leads to the development of diabetic nephropathy. The endothelial glycocalyx plays a critical role in glomerular barrier function. In the present study, the effects of piperazine ferulate (PF) on HG-induced filtration barrier injury of glomerular endothelial cells (GEnCs) were investigated and the underlying mechanism was assessed. Immunofluorescence was used to observe the distribution of the glycocalyx as well as the expression levels of syndecan-1 and Zonula occludens-1 (ZO-1). Endothelial permeability assays were performed to assess the effects of PF on the integrity of the filtration barrier. Protein and mRNA expression levels were measured by western blotting and reverse transcription-quantitative PCR analyses, respectively. In vitro experiments revealed that adenosine monophosphate-activated protein kinase (AMPK) mediated HG-induced glycocalyx degradation and endothelial barrier injury. PF inhibited the HG-induced endothelial barrier injury and restored the expression levels of heparanase-1 (Hpa-1), ZO-1 and occludin-1 by AMPK. In vivo assays demonstrated that PF reduced the expression levels of Hpa-1, increased the expression levels of ZO-1 and attenuated glycocalyx degradation in the glomerulus. These data suggested that PF attenuated HG-induced filtration barrier injury of GEnC by regulating AMPK expression.

Green Synthesis and Characterization of Silver Nanoparticles Using Azadirachta indica Seeds Extract: In Vitro and In Vivo Evaluation of Anti-Diabetic Activity

“…AI-AgNPs-treated group except for slight vacuolation and dilation of sinusoids (Figure 8). These results are in line with other mechanisms of interaction of nano Ag with cells that have been presented in the study [90], and various researchers have reported different approaches for diabetic medicine [91][92][93][94][95][96][97]. Considering the results obtained from antidiabetic activity, green-synthesised AgNPs are good anti-diabetic agents.…”

MDK Promotes Lymph Node Metastasis of Cervical Squamous Cell Carcinoma by Activating the PI3K/AKT and p38 MAPK Pathway

“…Syndecan-1 is one of the receptors for MDK and has been reported to regulate the expression of TJPs (ZO-1, occludin) [32]. We found that after siRNA interference with Syndecan-1 expression (Fig.…”

The association between dietary inflammatory index with some cardio-metabolic risk indices among the patients with type 2 diabetes from Hoveyzeh cohort study: a cross-sectional study

“…Inflammatory cytokines, like IL-1β and IL-6, can dampen the body’s ability to respond to insulin in fat, muscle, and liver. These cytokines are like pieces of a puzzle that explain how inflammation can lead to IR [ 68 , 69 ]. Prolonged high blood sugar due to IR directly damages blood vessel cells (endothelium, smooth muscle) and immune cells (macrophages), triggering abnormal blood clotting and tissue breakdown.…”