Ginsenoside Rg3 ameliorates allergic airway inflammation and oxidative stress in mice

Huang, Wen‐Chung; Huang, Tse-Hung; Yeh, Kuo Wei; Chen, Ya Ling; Shen, Sheng‐Feng; Liou, Chian-Jiun

doi:10.1016/j.jgr.2021.03.002

Cited by 46 publications

(34 citation statements)

References 43 publications

(65 reference statements)

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“…Our study shows that DIE is a potent anti-inflammatory agent that targets ROS to inhibit the NF κ B activation. This potential was also reported in Panax ginseng, which potently targets oxidative stress and the NF κ B [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 61%

Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice

Lee

Yuk

Saba

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. Duchesnea indica has been reported for its anti-inflammatory properties. However, its efficacy in sepsis has yet to be reported. In this study, we studied the ability of Duchesnea indica extract (DIE) to rescue mice from septic shock and sepsis. Methods. In vitro studies included the measurement of secreted nitric oxide, cell viability, gene and protein expression via real-time polymerase chain reaction and western blot, and confocal microscopy in RAW 264.7 cells. In vivo studies include a model of septic shock and sepsis in BALB/c mice induced by a lethal and sub-lethal dose of lipopolysaccharide (LPS). Results. DIE suppressed the expression of proinflammatory cytokines induced by LPS and prevented the translocation of NFκB into the nucleus of RAW 264.7 cells. It also prevented reactive oxygen species damage induced by LPS in murine bone marrow-derived macrophages. Models of sepsis and septic shock were established in BALB/c mice and DIE-rescued mice from septic shock. DIE also reversed the increase in tumor necrosis factor-α and nitrite levels in the serum of mice induced with sepsis. DIE also prevented the translocation of NFκB from the cytosol into the nucleus in murine lungs. Histopathological damage induced by sepsis was reversed in the testis, liver, and lungs of mice. Conclusion. In conclusion, DIE is a suitable candidate for development as a therapeutic agent for sepsis.

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Section: Discussionmentioning

confidence: 61%

Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice

Lee

Yuk

Saba

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…AHR was measured 24 h after the last OVA challenge, as described previously [ 49 ]. Briefly, mice were placed in a closed system to inhale aerosolized methacholine (0 to 40 mg/mL) for 3 min.…”

Section: Methodsmentioning

confidence: 99%

“…Lung tissues were homogenized, and a lipid peroxidation assay kit was used to detect MDA levels (Abcam, Waltham, MA, USA) as described previously [ 49 ]. Briefly, a lung tissue solution was incubated with the MDA color reagent solution, followed by incubation with reaction solution for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Sophoraflavanone G from Sophora flavescens Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model

Wang

Huang

Chen

et al. 2022

IJMS

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Sophoraflavanone G (SG), isolated from Sophora flavescens, has anti-inflammatory and anti-tumor bioactive properties. We previously showed that SG promotes apoptosis in human breast cancer cells and leukemia cells and reduces the inflammatory response in lipopolysaccharide-stimulated macrophages. We investigated whether SG attenuates airway hyper-responsiveness (AHR) and airway inflammation in asthmatic mice. We also assessed its effects on the anti-inflammatory response in human tracheal epithelial cells. Female BALB/c mice were sensitized with ovalbumin, and asthmatic mice were treated with SG by intraperitoneal injection. We also exposed human bronchial epithelial BEAS-2B cells to different concentrations of SG to evaluate its effects on inflammatory cytokine levels. SG treatment significantly reduced AHR, eosinophil infiltration, goblet cell hyperplasia, and airway inflammation in the lungs of asthmatic mice. In the lungs of ovalbumin-sensitized mice, SG significantly promoted superoxide dismutase and glutathione expression and attenuated malondialdehyde levels. SG also suppressed levels of Th2 cytokines and chemokines in lung and bronchoalveolar lavage samples. In addition, we confirmed that SG decreased pro-inflammatory cytokine, chemokine, and eotaxin expression in inflammatory BEAS-2B cells. Taken together, our data demonstrate that SG shows potential as an immunomodulator that can improve asthma symptoms by decreasing airway-inflammation-related oxidative stress.

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“…Subsequently, BALF was collected to measure the concentrations of cytokines and chemokines. BALF cells were stained with Giemsa stain to enable calculation of the types and number of immune cells [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…MDA activity and glutathione were measured with a lipid peroxidation assay kit and glutathione assay kit (Sigma), respectively. Briefly, lung tissues were homogenized and incubated with the appropriate reaction solution, as previously described [ 29 ]. MDA activity and glutathione levels were measured by use of a multi-mode microplate reader (Molecular Devices).…”

Section: Methodsmentioning

confidence: 99%

Gypenoside A from Gynostemma pentaphyllum Attenuates Airway Inflammation and Th2 Cell Activities in a Murine Asthma Model

Huang

Yeh

et al. 2022

IJMS

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Our previous study found that oral administration of Gynostemma pentaphyllum extract can attenuate airway hyperresponsiveness (AHR) and reduce eosinophil infiltration in the lungs of asthmatic mice. Gypenoside A is isolated from G. pentaphyllum. In this study, we investigated whether gypenoside A can effectively reduce asthma in mice. Asthma was induced in BALB/c mice by ovalbumin injection. Asthmatic mice were treated with gypenoside A via intraperitoneal injection to assess airway inflammation, AHR, and immunomodulatory effects. In vitro, gypenoside A reduced inflammatory and oxidative responses in inflammatory tracheal epithelial cells. Experimental results showed that gypenoside A treatment can suppress eosinophil infiltration in the lungs, reduce tracheal goblet cell hyperplasia, and attenuate AHR. Gypenoside A significantly reduced Th2 cytokine expression and also inhibited the expression of inflammatory genes and proteins in the lung and bronchoalveolar lavage fluid. In addition, gypenoside A also significantly inhibited the secretion of inflammatory cytokines and chemokines and reduced oxidative expression in inflammatory tracheal epithelial cells. The experimental results suggested that gypenoside A is a natural compound that can effectively reduce airway inflammation and AHR in asthma, mainly by reducing Th2 cell activation.

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Ginsenoside Rg3 ameliorates allergic airway inflammation and oxidative stress in mice

Cited by 46 publications

References 43 publications

Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice

Duchesnea indica Extract Ameliorates LPS-Induced Septic Shock in Mice

Sophoraflavanone G from Sophora flavescens Ameliorates Allergic Airway Inflammation by Suppressing Th2 Response and Oxidative Stress in a Murine Asthma Model

Gypenoside A from Gynostemma pentaphyllum Attenuates Airway Inflammation and Th2 Cell Activities in a Murine Asthma Model

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