Ginsenoside Ro suppresses interleukin-1β-induced apoptosis and inflammation in rat chondrocytes by inhibiting NF-κB

Zhang, Xiaohong; Xu, Xin; Xu, Tao

doi:10.1016/s1875-5364(15)30015-7

Cited by 50 publications

(50 citation statements)

References 30 publications

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“…Rg1 inhibited IL-1β induced chondrocyte apoptosis, promoted TIMP-1 expression and inhibited MMP-13 expression via its effects on the phosphatidylinositol-3-kinase/protein kinase B and mitochondrial signaling pathways (31). Furthermore, Ro evidently prevented IL-1β-stimulated chondrocyte apoptosis and inflammation by inhibiting nuclear factor-κB activation (32).…”

Section: Discussionmentioning

confidence: 97%

“…'Shen' and 'mai' are Chinese abbreviations for red ginseng and Ophiopogon japonicas. SMI contains a number of gensenosides from red ginseng, considered to be beneficial in the protection of articular cartilage, thus it is worth investigating whether SMI has the potential to treat KOA (27)(28)(29)(30)(31)(32). The aim of the present study was to evaluate the anti-KOA activities of SMI on knee articular cartilage in an experimental rabbit KOA model induced by anterior cruciate ligament transection (ACLT), as well as in IL-1β-stimulated human chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of Shenmai injection on knee articular cartilage of osteoarthritic rabbits and IL-1β-stimulated human chondrocytes

Yao

Chen

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Shenmai injection (SMI) has been widely used as a therapy to treat a number of diseases. However, its anti-osteoarthritic properties have not yet been fully investigated. In the present study, the protective effect of SMI on knee articular cartilage of anterior cruciate ligament transected rabbits and interleukin-1β (IL-1β)-stimulated human chondrocytes was investigated. For the in vivo study, knee osteoarthritis (KOA) was induced in female New Zealand white rabbits by anterior cruciate ligament transection (ACLT) in the knee of right hind limb. Rabbits either underwent sham surgery or ACLT surgery. Out of the rabbits receiving ACLT surgery, half of the rabbits received one 0.3 ml Shenmai intra-articular injection in the knee per week for four weeks, following ACLT surgery. The other rabbits received the same volume of normal saline solution. The cartilage was subsequently collected for histological evaluation. For the in vitro study, cultured human chondrocytes were treated with 10 ng/ml IL-1β in the presence or absence of 5 and 2% (v/v) SMI for 24 h. Nitric oxide (NO) and prostaglandin E2 (PGE2) levels in cell culture supernatant were assessed using a Griess reaction and ELISA respectively.The mRNA expression of cyclooxgenase-2 (COX-2), inducible nitric oxide synthase (iNOS), matrix metalloproteinase (MMP)-1, MMP-13 and tissue inhibitors of metalloproteinase-1 (TIMP-1) in chondrocytes were detected by reverse transcription-quantitative polymerase chain reaction. The results of the current study revealed that treatment with SMI ameliorated cartilage degradation in the ACLT rabbit model, and decreased levels of NO and PGE2. Furthermore, treatment with SMI decreased levels of COX-2, iNOS, MMP-1 and MMP-13 mRNA expression and increased TIMP-1 mRNA expression in IL-1β-stimulated human chondrocytes. These results indicate that SMI suppresses inflammation and ameliorated cartilage degradation, making it a potential and promising therapeutic option to treat KOA.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Protective effect of Shenmai injection on knee articular cartilage of osteoarthritic rabbits and IL-1β-stimulated human chondrocytes

Yao

Chen

et al. 2017

Experimental and Therapeutic Medicine

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“…17 As previously reported, treating chondrocytes with IL-1β could reduce chondrocyte viability and upregulate apoptotic-related proteins, and several anti-inflammatory agents had been believed to exert anti-apoptosis effects in chondrocytes. 13,18 The dose of GB used in different cells varies from 25−1000 μΜ, and in our study, we demonstrated that GB exerted anti-inflammation and anti-apoptosis effects in chondrocytes at doses of 50−100 μΜ. 7,10,12 Previous studies have suggested that LPS markedly induces upregulation of genes and the generation of various matrix-degrading enzymes and proinflammatory cytokines, including IL-6, Cox-2, ADAMTS-5, ADAMTS-4, MMP-13 and MMP-3, in chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Ginkgolide B exerts anti-inflammatory and chondroprotective activity in LPS-induced chondrocytes

Hu¹,

Li²,

Xin³

et al. 2018

Adv Clin Exp Med

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“…2). Previous researches on ginsenoside Ro mostly involved in the anti-inflammatory 28, 29 , thrombosis prevention 30, 31 and anti-oxidation 32 . The most eye-catching newly discovery of ginsenoside Ro is its autophagy inhibition activity, which beyond several other ginsenosides extraceted form Panax ginseng 33 .…”

Section: Discussionmentioning

confidence: 99%

The traditional Chinese medicine Achyranthes bidentata and our de novo conception of its metastatic chemoprevention: from phytochemistry to pharmacology

Zhou

Qian

Dong

et al. 2017

Sci Rep

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Our recent biosystems analysis revealed similarities between embryonic implantation and cancer cell adhesion, which suggests that abortifacients may be good for safe and effective metastatic chemoprevention targeting circulating tumor cells (CTC). Here we test the hypothesis by using the well-known abortion herb Achyranthes bidentata Blume (A. bidentata). Five compounds were separated from the herb root. Among them, ginsenoside Ro was the most potent in inhibiting embryonic implantation within non-cytotoxic concentrations. It specifically inhibited the metastatic dissemination capability of colon cancer cells HT29, including the migration and invasion ability, and their adhesion to human endothelium through inhibiting integrin αvβ6, MMP-2, MMP-9, and ERK phosphorylation by HT29. Pretreatment of nude mice with oral ginsenoside Ro followed by HT29 intravenous inoculation and 40-day oral ginsenoside Ro significantly prevented lung metastasis with downregulation of integrin αvβ6 and no toxicity. The present study firstly introduces the new conception of utilizing safe and effective abortion botanic medicines for CTC-based metastatic chemoprevention.

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Ginsenoside Ro suppresses interleukin-1β-induced apoptosis and inflammation in rat chondrocytes by inhibiting NF-κB

Cited by 50 publications

References 30 publications

Protective effect of Shenmai injection on knee articular cartilage of osteoarthritic rabbits and IL-1β-stimulated human chondrocytes

Protective effect of Shenmai injection on knee articular cartilage of osteoarthritic rabbits and IL-1β-stimulated human chondrocytes

Ginkgolide B exerts anti-inflammatory and chondroprotective activity in LPS-induced chondrocytes

The traditional Chinese medicine Achyranthes bidentata and our de novo conception of its metastatic chemoprevention: from phytochemistry to pharmacology

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