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Beena, M. R.; Martin, K. P.; Kirti, P. B.; Hariharan, Molly

doi:10.1023/a:1022395809204

Cited by 70 publications

(7 citation statements)

References 9 publications

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“…1d, 2). Although several authors have reported a synergistic effect between cytokinins and auxins during the proliferation of various species (Beena et al 2003;Faisal et al 2007), in this study, this positive effect was not observed when KIN and IBA were used together. In fact, this combination was less effective than the application of KIN alone in both 1/2MS (4.86 ± 0.40 and 4.16 ± 0.36 shoots per culture for 0.5 mg l -1 KIN + 0.25 mg l -1 IBA and 0.5 mg l -1 KIN + 0.5 mg l -1 IBA, respectively) and 1/4MS medium (7.37 ± 0.53 and 5.38 ± 0.54, respectively).…”

contrasting

confidence: 77%

An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant

Gonçalves

Escapa

Grevenstuk

et al. 2008

Plant Cell Tiss Organ Cult

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In this study, an efficient protocol was developed for in vitro propagation of Pinguicula lusitanica L., a rare insectivorous plant with pharmacological value and limited reproductive capacity. The effects of two concentrations (0.1 and 0.5 mg l -1 ) of a range of plant growth regulators, including cytokinins (BA, KIN, and ZEA) and auxins (IAA, IBA, and NAA), and three concentrations of MS medium macronutrients (total, 1/2 and 1/4MS) on proliferation and rooting, were investigated. P. lusitanica shoots showed abundant proliferation and rooting capacity, both of which were significantly influenced by MS medium strength. The use of 1/2MS supplemented with 0.5 mg l -1 BA or KIN ensure a 29-fold rate of proliferation. Best rooting frequency and higher root number and length were attained in 1/4MS medium containing 0.2 mg l -1 IAA. Sixty percent of the plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development. These plantlets can be used to replenish declining populations in the wild and/or for the extraction of bioactive compounds, reducing pressure on wild stocks.

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contrasting

confidence: 77%

An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant

Gonçalves

Escapa

Grevenstuk

et al. 2008

Plant Cell Tiss Organ Cult

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“…It therefore appeared that BA and NAA have synergistic effects on shoot multiplication. The synergistic effect of BA in combination with an auxin has been reported for many Asclepiadaceae medicinal plants such as Holostemma annulare (Sudha et al 1998), Hemidesmus indicus (Sreekumar et al 2000, Holostemma ada-kodien (Martin 2002), and Ceropegia candelabrum (Beena et al 2003). Increased NAA concentration resulted in an increase in the number of roots produced per explant (Table 1).…”

Section: Shoot and Root Organogenesismentioning

confidence: 66%

“…The efficiency of half strength MS PGR-free medium and/ or IBA in rooting has been reported for many Asclepiadaceae plants (Komalavalli and Rao 2000;Sreekumar et al 2000;Martin 2002;Beena et al 2003). Patnaik and Debata (1996) further observed that IBA was more effective than IAA for the induction of rooting in Hemidesmus indicus.…”

Section: Rooting and Acclimatizationmentioning

confidence: 79%

In vitro propagation of Huernia hystrix: an endangered medicinal and ornamental succulent

Amoo

Finnie

Staden

2009

Plant Cell Tiss Organ Cult

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An efficient and rapid method for in vitro clonal propagation of Huernia hystrix was developed, resulting in shoot regeneration within 3 weeks of culture. This endangered medicinal and ornamental succulent is in high demand. Multiple shoots were regenerated from stem explants (10 mm length) cultured on Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with a range of NAA (0.00-8.06 lM) and BA (4.44-22.19 lM) concentrations. A 100% shoot response with a multiplication rate of four shoots per explant was obtained on MS medium containing 5.37 lM NAA and 22.19 lM BA. Callus produced at the base of the explant on the same medium showed root organogenic potential. The in vitro regenerated shoots produced roots when transferred to half strength MS medium with or without auxin. The micropropagated plants were easily acclimatized within 2 months under greenhouse conditions when potted in a soil and sand mixture (1:1; v/v) treated with a fungicide (Benlate, 0.01%). More than 95% survival with no observable morphological variations was obtained. The developed protocol provides a simple, cost-effective means for the conservation of endangered H. hystrix by clonal propagation within a short time.

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“…1a). Similarly, efficacy of BAP towards production of multiple shoots was reported for C. candelabrum [9] and C. noorjahaniae [10]. TDZ (2.0 mg/l) produced 3.2 ± 0.2 numbers of shoots, and it was observed that the increased concentrations resulted in less number of shoots as well as stunted growth.…”

Section: Resultsmentioning

confidence: 55%

In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Adsul

Chavan²,

Gaikwad

et al. 2019

Journal of Genetic Engineering and Biotechnology

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The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.

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Untitled

Cited by 70 publications

References 9 publications

An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant

An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant

In vitro propagation of Huernia hystrix: an endangered medicinal and ornamental succulent

In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

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