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Yokoi, Shuji; Higashi, Sho Ichi; Kishitani, Sachie; Murata, Norio; Toriyama, Kinya

doi:10.1023/a:1009671231614

Cited by 68 publications

(6 citation statements)

References 18 publications

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“…Genetic engineering of the unsaturation of fatty acids has been achieved by manipulation of the cDNA for the GPAT found in chloroplasts and has allowed modification of the ability of tobacco to tolerate chilling temperatures [ 123 ]. Introduction of the cDNA for shape Arabidopsis glycerol-3-phosphate acyltransferase (GPAT) confers unsaturation of fatty acids and chilling tolerance of photosynthesis on rice [ 124 ]. Xu et al [ 125 ] suggest that leaf dehydration tolerance and post-drought recovery in Kentucky bluegrass was associated with their ability to maintain relative higher proportion and level of unsaturated fatty acids.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment

Zhang

Gao

et al. 2021

PLoS ONE

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Kentucky bluegrass (Poa pratensis L.) is an excellent cool-season turfgrass utilized widely in Northern China. However, turf quality of Kentucky bluegrass declines significantly due to drought. Ethephon seeds-soaking treatment has been proved to effectively improve the drought tolerance of Kentucky bluegrass seedlings. In order to investigate the effect of ethephon leaf-spraying method on drought tolerance of Kentucky bluegrass and understand the underlying mechanism, Kentucky bluegrass plants sprayed with and without ethephon are subjected to either drought or well watered treatments. The relative water content and malondialdehyde conent were measured. Meanwhile, samples were sequenced through Illumina. Results showed that ethephon could improve the drought tolerance of Kentucky bluegrass by elevating relative water content and decreasing malondialdehyde content under drought. Transcriptome analysis showed that 58.43% transcripts (254,331 out of 435,250) were detected as unigenes. A total of 9.69% (24,643 out of 254,331) unigenes were identified as differentially expressed genes in one or more of the pairwise comparisons. Differentially expressed genes due to drought stress with or without ethephon pre-treatment showed that ethephon application affected genes associated with plant hormone, signal transduction pathway and plant defense, protein degradation and stabilization, transportation and osmosis, antioxidant system and the glyoxalase pathway, cell wall and cuticular wax, fatty acid unsaturation and photosynthesis. This study provides a theoretical basis for revealing the mechanism for how ethephon regulates drought response and improves drought tolerance of Kentucky bluegrass.

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Section: Resultsmentioning

confidence: 99%

Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment

Zhang

Gao

et al. 2021

PLoS ONE

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“…Three types of GPATs have been found in plant cells, which are located in the plastids (including chloroplasts), endoplasmic reticulum, and mitochondria ( Murata and Tasaka, 1997 ; Zheng et al, 2003 ; Gidda et al, 2009 ). Among these, GPATs in plastids/chloroplasts are soluble proteins, which affect plants’ cold tolerance by affecting the saturation of chloroplast membrane lipid molecules ( Murata and Tasaka, 1997 ; Yokoi et al, 1998 ; Zhu et al, 2009 ; Sui et al, 2017 ). GPATs distributed in the endoplasmic reticulum and mitochondria are membrane-bound proteins, which participate in the biosynthesis of triacylglycerol (TAGs) in seeds, thereby affecting the accumulation of seed oil ( Zheng et al, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

GWAS and RNA-seq analysis uncover candidate genes associated with alkaline stress tolerance in maize (Zea mays L.) seedlings

Jia

Zhou

et al. 2022

Front. Plant Sci.

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Soil salt-alkalization is a common yet critical environmental stress factor for plant growth and development. Discovering and exploiting genes associated with alkaline tolerance in maize (Zea mays L.) is helpful for improving alkaline resistance. Here, an association panel consisting of 200 maize lines was used to identify the genetic loci responsible for alkaline tolerance-related traits in maize seedlings. A total of nine single-nucleotide polymorphisms (SNPs) and their associated candidate genes were found to be significantly associated with alkaline tolerance using a genome-wide association study (GWAS). An additional 200 genes were identified when the screen was extended to include a linkage disequilibrium (LD) decay distance of r2 ≥ 0.2 from the SNPs. RNA-sequencing (RNA-seq) analysis was then conducted to confirm the linkage between the candidate genes and alkali tolerance. From these data, a total of five differentially expressed genes (DEGs; |log2FC| ≥ 0.585, p < 0.05) were verified as the hub genes involved in alkaline tolerance. Subsequently, two candidate genes, Zm00001d038250 and Zm00001d001960, were verified to affect the alkaline tolerance of maize seedlings by qRT-PCR analysis. These genes were putatively involved protein binding and “flavonoid biosynthesis process,” respectively, based on Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses. Gene promoter region contains elements related to stress and metabolism. The results of this study will help further elucidate the mechanisms of alkaline tolerance in maize, which will provide the groundwork for future breeding projects.

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“…Nipponbare by PCR with a primer pair (FAPGbam 5′-CGG GAT CCA TGC TAC GCG GGA ACG ACAC-3′ and RAPGbam 5′-CGG GAT CCT CAC GCC TGC TTC ACG GCGG-3′) and sub-cloned to pBI101-H-Ub binary vector at BamHI restriction site ( Figure 1A) (Yokoi et al 1998). e binary vector construct was introduced to Agrobacterium strain EHA105.…”

Section: Vector Construction For Plant Transformation and Phenotype Omentioning

confidence: 99%

Overexpression of a basic helix–loop–helix gene Antagonist of PGL1 (APG) decreases grain length of rice

Heang

Sassa

2012

Plant Biotechnology

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Previously we reported that three basic helix-loop-helix (bHLH) protein dimers are involved in regulating grain length of rice. Two atypical bHLH proteins named P (PGL1) and PGL2 positively regulate grain length in transgenic rice when they are overexpressed in lemma/palea. eir interaction partner A PGL (APG), a typical bHLH, is assumed to have opposite function. Suppression of APG expression level by RNAi increased grain length signi cantly. Here, we report further evidence that APG is a negative regulator of grain length in rice. Overexpression of APG resulted in short grain. Inner epidermal cell layer of lemma from APG overexpression lines showed reduced cell length. Taken together, the results illustrated the biological role of a typical bHLH APG as a negative regulator of rice grain length that controls cell elongation. Key words:Basic helix-loop-helix, cell elongation, grain length, rice.Like other cereal crops, rice yield partly depends on the grain size (Mao et al. 2010). At least four parameters are considered to contribute to the rice grain size: grain length, width, thickness and lling ability (Xing and Zhang 2010). Although these important traits are also major objectives of many breeding programs for yield improvement in rice, the genetic and molecular studies on rice grain size remain limited. Only a few genes have been characterized for grain length, width and grain lling to date (Kitagawa et al. 2010;Li et al. 2011;Shomura et al. 2008;Song et al. 2007; TakanoKai et al. 2009;Wang et al. 2008). Among these studies, one of the research focus is lemma and palea covering the endosperm to compose a rice grain. e size of the grain have been demonstrated to be partly limited by the size of lemma and palea (Shomura et al. 2008;Song et al. 2007; Heang and Sassa 2012). erefore, uncovering the molecular mechanism controlling lemma/palea size is expected to contribute the understanding of regulation of grain size in rice.Basic helix-loop-helix (bHLH) protein is a large family of transcription factors in plant (Feller et al. 2011). 167 and 177 genes are predicted to encode the class of proteins in Arabidopsis and rice, respectively Because of the lack of the conserved amino acid residues, some proteins are considered to be unable to bind DNA, and grouped to atypical bHLH proteins (Li et al. 2006). HLH domain is required to form protein dimers. A typical bHLH protein retains both DNA binding and protein dimerization domains. Recent studies have revealed the involvement of the bHLH transcription factor in diverse plant growth and development processes such as, light signaling (Fairchild et al. 2000;Khanna et al. 2004), hormone signaling (Schlereth et al. 2010Tanaka et al. 2009;Zhang et al. 2009), root development (Yi et al. 2010), fruit and ower development (Pen eld et al. 2005; Szecsi et al. 2006) and drought response (Seo et al. 2010). More importantly, many of bHLH genes are reported to be involved in organ size and cell elongation (de Lucas et al. 2008;Fairchild et al. 2000;Mara et al. 2010;Varaud...

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Untitled

Cited by 68 publications

References 18 publications

Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment

Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment

GWAS and RNA-seq analysis uncover candidate genes associated with alkaline stress tolerance in maize (Zea mays L.) seedlings

Overexpression of a basic helix–loop–helix gene Antagonist of PGL1 (APG) decreases grain length of rice

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