GLAD-PCR assay of DNA methylation sites in regulatory regions of some tumor-suppressor genes in breast cancer

Abstract

Hypermethylation of the RcgY sites is shown for many cancer diseases. such aberrant methylation, suppressing the gene activity, occurs at early stages of carcinogenesis. Recently, using glad-pcR assay, we have detected aberrantly methylated RcgY sites, which can be considered to be epigenetic markers of colorectal, lung, and gastric cancers. in breast cancer, methylation of the regulatory regions of ALX4, BMP2, CCND2, CDH13, CDX1, FOXA1, GALR1, GATA5, GREM1, HIC1, HMX2, HS3ST2, HOXC10, ICAM5, LAMA1, RARB, RASSF1A, RUNX3, RXRG, RYR2, SFRP2, SOX17, TERT, and ZNF613 tumor-suppressor genes is reported. in the present work, we determined aberrantly methylated RcgY sites in the regulatory regions of these genes in dNa preparations from breast cancer tissues. the study of dNa samples from 30 tumor and 22 normal mammary tissue samples demonstrates a high diagnostic potential of selected R(5mc)gY sites in regulatory regions of CCND2, BMP2, GALR1, SOX17, HMX2, and HS3ST2 genes with total index of sensitivity and specificity for R(5mc)gY detection in tumor dNa 90.0 % and 100.0 %, respectively.

Immune-related gene signature associates with immune landscape and predicts prognosis accurately in patients with skin cutaneous melanoma

“…In addition, Schulten et al (2015) showed that RXRG was significantly upregulated in follicular FVPTCs of papillary thyroid carcinoma. Smetannikova et al (2019) reported that results of differential expression in DNA samples from lung cancer versus normal lung tissue showed that RXRG had high sensitivity and specificity and possessed good diagnostic potential. This is consistent with our findings, indicating that RXRG is essential in the advancement of SKCM.…”

PAX9 functions as a tumor suppressor gene for cervical cancer via modulating cell proliferation and apoptosis

“…(1) Clinicopathological features included in this study should be further comprehensively summarized and (2) the possible mechanism, which suggested that the abnormal expression of PAX9 in CC may be correlated with the methylation, [35][36][37] can only be further explored in the follow-up research due to the limited time and funds.…”

“…These results indicated that PAX9, possibly via inhibiting the proliferation and accelerating the apoptosis of tumor cells, can inhibit the growth of tumor. However, this study still has some limitations: (1) Clinicopathological features included in this study should be further comprehensively summarized and (2) the possible mechanism, which suggested that the abnormal expression of PAX9 in CC may be correlated with the methylation, 35–37 can only be further explored in the follow‐up research due to the limited time and funds.…”