GLC Assay of Meprobamate and Related Carbarnates

Wall-coated open tubular column coupled with nitrogen-selective detector for routine glc determination of diazepam, meprobamate, phenylbutazone, and thioridazine in serum

“…The high resolution of the capillary column (23) reduced the extraction stage to a single step instead of the three steps often suggested to eliminate the interfering substances (12, 13, 15, 17). Nevertheless, the accuracy and sensitivity were usually improvements over previous assays (3)(4)(5)9,11,12,15,17). The rapid elution time (24) shortened the retention times.…”

Immobilization of proteins in microspheres of biodegradable polyacryldextran

“…In addition, other experimentsg with 11-1-75 particles showed that polyacryldextran was metabolized more rapidly in uitro with rat liver homogenates and also was eliminated faster in uiuo after intraperitoneal injection in mice than in the cases involving polyacrylamide (T-C value of [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. These findings were expected since dextran should be degraded easier in uiuo than hydrocarbon chains, the content of which is comparatively small in polyacryldextran particles with D-T-C values of 11-1-75.…”

“…The high resolution of the capillary column (23) reduced the extraction stage to a single step instead of the three steps often suggested to eliminate the interfering substances (12,13, 15,17). Nevertheless, the accuracy and sensitivity were usually improvements over previous assays (3)(4)(5)9, 11,12,15,17). The rapid elution time (24) shortened the retention times.…”

“…The high resolution of the capillary column (23) reduced the extraction stage to a single step instead of the three steps often suggested to eliminate the interfering substances (12,13, 15,17). Nevertheless, the accuracy and sensitivity were usually improvements over previous assays (3)(4)(5)9, 11,12,15,17).The determination in serum of four drugs useful in toxicology (25-29) or therapeutic monitoring (30-32) demonstrated the versatility and potential of this chromatographic system and its possible routine use in view of the growing importance of serum level determinations of an increasing number of diverse drugs in clinical practice.The method was demonstrated by the determination of serum concentrations of diazepam in five epileptic patients (Table IV), of meprobamate in four cases of voluntary overdose (Table V), and of phenylbutazone in a nursing mother given a 250-mg suppository twice daily for 10 days (Table VI).Abstract 0 Macromolecules were immobilized by an emulsion polymerization technique in biodegradahle microspheres of polyacryldextran, prepared by copolymerizing hisacrylamide with acryldextran. The rapid elution time (24) shortened the retention times.…”

“…The amount of proteins leaking out from the microparticles was determined according t o the method of Lowry et al (9). The amount of proteins leaking out from the microparticles was determined according t o the method of Lowry et al (9).…”