GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function.

Murphy, Robert A.; Watkins, Janis L.; Wente, Susan R.

doi:10.1091/mbc.7.12.1921

Cited by 168 publications

(177 citation statements)

References 101 publications

(148 reference statements)

Supporting

Mentioning

164

Contrasting

Order By: Relevance

“…Antibodies-MBP-Gle1 was expressed and purified as described previously (37). The antigen was sent to Cocalico Biologicals for production of rabbit antiserum WU851.…”

Section: Methodsmentioning

confidence: 99%

“…The antigen was sent to Cocalico Biologicals for production of rabbit antiserum WU851. Antiserum to Gle1 was purified by affinity chromatography over a GST-Gle1 Affi-Gel 10 column (Bio-Rad) as described previously (37). Anti-Gfd1 peptide antibodies (Gfd1D/1) were generated by Bethyl Laboratories.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, Mex67 is a key player in connecting transcription and mRNA export. Other non-hnRNP factors with roles in mRNA export are Dbp5, Gle1, and Gle2/Rae1 (33)(34)(35)(36)(37)(38). Dbp5 is a DEAD box RNA helicase that localizes to the cytoplasmic face of the NPC through an interaction with Nup159 (39,40).…”

mentioning

confidence: 99%

“…Recent work (41) has demonstrated that Dbp5 also has a role in transcription. Gle2 is required for efficient mRNA export in yeast and has a docking site on Nup116 (37,42,43). The role of Gle2/Rae1 in vertebrate cells is not clear because mouse embryo fibroblasts and blastocytes from knock out mice exhibit cell cycle defects but not mRNA export defects (44).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Nuclear Export of the Yeast mRNA-binding Protein Nab2 Is Linked to a Direct Interaction with Gfd1 and to Gle1 Function

Suntharalingam¹,

Alcázar-Román

Wente

2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

Nuclear export of mRNA is mediated by interactions between soluble factors and nuclear pore complex (NPC) proteins. In Saccharomyces cerevisiae, Nab2 is an essential RNA-binding protein that shuttles between the nucleus and cytoplasm. The mechanism for trafficking of Nab2-bound mRNA through the NPC has not been defined. Gle1 is also required for mRNA export, and Gle1 interactions with NPC proteins, the RNA helicase Dbp5, and Gfd1 have been reported. Here we report that Nab2, Gfd1, and Gle1 associate in a complex. By using immobilized recombinant Gfd1, Nab2 was isolated from total yeast lysate. A similar biochemical assay with immobilized recombinant Nab2 resulted in coisolation of Gfd1 and Gle1. A Nab2-Gfd1 complex was also identified by coimmunoprecipitation from yeast lysates. In vitro binding assays with recombinant proteins revealed a direct association between Nab2 and Gfd1, and two-hybrid assays delineated Gfd1 binding to the N-terminal Nab2 domain. This N-terminal Nab2 domain is distinct from its RNA binding domains suggesting Nab2 could bind Gfd1 and RNA simultaneously. As Nab2 export was blocked in a gle1 mutant at the restrictive temperature, we propose a model wherein Gfd1 serves as a bridging factor between Gle1 and Nab2-bound mRNA during export.

show abstract

“…Antibodies-MBP-Gle1 was expressed and purified as described previously (37). The antigen was sent to Cocalico Biologicals for production of rabbit antiserum WU851.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Nuclear Export of the Yeast mRNA-binding Protein Nab2 Is Linked to a Direct Interaction with Gfd1 and to Gle1 Function

Suntharalingam¹,

Alcázar-Román

Wente

2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Plasmid pSW896 (ADE3 URA3 CEN; Murphy et al, 1996) was cut with NotI, treated with Klenow, and religated to destroy the NotI site, creating pABS28. The YOP1 genomic region was amplified with the primers 5Ј-ATATATGGA TCCTTGAAAAACTGTGGAGCC-3Ј and 5Ј-TATATAGGATCCTCTTCCAC-AATAAACGCC-3Ј.…”

Section: Dna Constructs and Manipulationsmentioning

confidence: 99%

Function of a Plant Stress-Induced Gene, HVA22. Synthetic Enhancement Screen with Its Yeast Homolog Reveals Its Role in Vesicular Traffic

Brands

2002

Plant Physiology

View full text Add to dashboard Cite

Expression of the barley (Hordeum vulgare) HVA22 gene is induced by environmental stresses, such as dehydration, salinity, and extreme temperatures, and by a plant stress hormone, abscisic acid. Genes sharing high level of sequence similarities with HVA22 exist in diverse eukaryotic organisms, including animals, plants, and fungi, but not in any prokaryotic organisms. The yeast (Saccharomyces cerevisiae) HVA22 homolog, Yop1p, has been shown to interact with the GTPaseinteracting protein, Yip1p. Deletion of YOP1 led to only a modest reduction of the stationary phase titer at 37C. A synthetic enhancement mutant screen was performed in the yop1 deletion background to identify genes interacting with YOP1. The open reading frame YOR165W (renamed SEY1 for synthetic enhancement of YOP1) was identified as a YOP1-dependent complementation gene. The yeast SEY1 is a homolog of the Arabidopsis RHD3 gene whose mutations cause the accumulation of transport vesicles near the tips of defective root hairs. The yeast double mutant of yop1 and sey1 is defective in vesicular traffic as evidenced by the accumulation of transport vesicles and the decrease in invertase secretion. Based on these observations, we suggest that Yop1p/HVA22 regulates vesicular traffic in stressed cells either to facilitate membrane turnover, or to decrease unnecessary secretion.Sessile organisms such as plants and fungi have developed sophisticated responses to environmental stresses that allow them to tolerate adverse conditions (Hohmann and Willem, 1997;Hoekstra et al., 2001). One approach to understanding these responses is through the identification of genes that are up-regulated during abiotic stress, followed by functional analyses of the corresponding gene products. In plants, stress stimulates the production of the phytohormone abscisic acid (ABA) (Zeevaart and Creelmann, 1988; Bray, 2002), which induces the expression of a variety of genes (Chandler and Robertson, 1994; Bray, 2002). Furthermore, elevated levels of ABA are correlated with late embryogenesis and the onset and maintenance of seed dormancy (Zeevaart and Creelmann, 1988). Because the dehydration of the seed during late embryogenesis is a normal part of the developmental program, these tissues provide a valuable resource for the identification of genes that are involved in desiccation tolerance.In cereals, a metabolically active tissue, the aleurone layer, surrounds the starchy endosperm of the seed. Upon germination, the embryo produces the phytohormone GA, which induces the production of hydrolytic enzymes by the aleurone tissue. These enzymes are secreted into the endosperm, where they liberate sugars and amino acids for the growing embryo. ABA blocks the production of these enzymes at the transcriptional level (Lovegrove and Hooley, 2000). The gene HVA22 was originally identified as a transcript that accumulates in barley (Hordeum vulgare) aleurone tissue upon treatment with ABA, and was later found to be induced in vegetative tissues exposed to ABA, drought, or cold stress (Shen et...

show abstract

Mechanisms of nuclear pore complex assembly – two different ways of building one molecular machine

2017

View full text Add to dashboard Cite

The nuclear pore complex (NPC) mediates all macromolecular transport across the nuclear envelope. In higher eukaryotes that have an open mitosis, NPCs assemble at two points in the cell cycle: during nuclear assembly in late mitosis and during nuclear growth in interphase. How the NPC, the largest nonpolymeric protein complex in eukaryotic cells, self‐assembles inside cells remained unclear. Recent studies have started to uncover the assembly process, and evidence has been accumulating that postmitotic and interphase NPC assembly use fundamentally different mechanisms; the duration, structural intermediates, and regulation by molecular players are different and different types of membrane deformation are involved. In this Review, we summarize the current understanding of these two modes of NPC assembly and discuss the structural and regulatory steps that might drive the assembly processes. We furthermore integrate understanding of NPC assembly with the mechanisms for rapid nuclear growth in embryos and, finally, speculate on the evolutionary origin of the NPC implied by the presence of two distinct assembly mechanisms.

show abstract

GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function.

Cited by 168 publications

References 101 publications

Nuclear Export of the Yeast mRNA-binding Protein Nab2 Is Linked to a Direct Interaction with Gfd1 and to Gle1 Function

Nuclear Export of the Yeast mRNA-binding Protein Nab2 Is Linked to a Direct Interaction with Gfd1 and to Gle1 Function

Function of a Plant Stress-Induced Gene, HVA22. Synthetic Enhancement Screen with Its Yeast Homolog Reveals Its Role in Vesicular Traffic

Mechanisms of nuclear pore complex assembly – two different ways of building one molecular machine

Contact Info

Product

Resources

About