Gliadin Electrophoretic Analysis of the Genetic Integrity of Wheat (Triticum aestivum L.) Accessions After Frequent Seed Reproductions

Konarev, Alekseij; Gubareva, N. K.; Kornuchin, Dimitri; Börner, Andreas

doi:10.1007/s10722-005-4674-2

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…Many genebank accessions and cultivars are not composed of genetically uniform germplasms [ 34 , 44 , 45 ]. Accessions of landraces are most often a mixture of hexaploid T. aestivum and tetraploid T. turgidum group durum genotypes, as was evident for 'Sinde' CGN12041 in this study.…”

Section: Discussionmentioning

confidence: 99%

Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

Salentijn¹,

Goryunova

Bas

et al. 2009

BMC Genomics

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BackgroundΑ-gliadins form a multigene protein family encoded by multiple α-gliadin (Gli-2) genes at three genomic loci, Gli-A2, Gli-B2 and Gli-D2, respectively located on the homoeologous wheat chromosomes 6AS, 6BS, and 6DS. These proteins contain a number of important celiac disease (CD)-immunogenic domains. The α-gliadins expressed from the Gli-B2 locus harbour fewer conserved CD-epitopes than those from Gli-A2, whereas the Gli-D2 gliadins have the highest CD-immunogenic potential. In order to detect differences in the highly CD-immunogenic α-gliadin fraction we determined the relative expression level from the homoeologous Gli-2 loci in various tetraploid and hexaploid wheat genotypes by using a quantitative pyrosequencing method and by analyzing expressed sequence tag (EST) sequences.ResultsWe detected large differences in relative expression levels of α-gliadin genes from the three homoeologous loci among wheat genotypes, both as relative numbers of expressed sequence tag (EST) sequences from specific varieties and when using a quantitative pyrosequencing assay specific for Gli-A2 genes. The relative Gli-A2 expression level in a tetraploid durum wheat cultivar ('Probstdorfer Pandur') was 41%. In genotypes derived from landraces, the Gli-A2 frequency varied between 12% and 58%. In some advanced hexaploid bread wheat cultivars the genes from locus Gli-B2 were hardly expressed (e.g., less than 5% in 'Lavett') but in others they made up more than 40% (e.g., in 'Baldus').ConclusionHere, we have shown that large differences exist in relative expression levels of α-gliadins from the homoeologous Gli-2 loci among wheat genotypes. Since the homoelogous genes differ in the amount of conserved CD-epitopes, screening for differential expression from the homoeologous Gli-2 loci can be employed for the pre-selection of wheat varieties in the search for varieties with very low CD-immunogenic potential. Pyrosequencing is a method that can be employed for such a 'gene family-specific quantitative transcriptome profiling'.

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Section: Discussionmentioning

confidence: 99%

Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

Salentijn¹,

Goryunova

Bas

et al. 2009

BMC Genomics

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“…Similarly, gliadin blocks may differ in intensity, electrophoretic mobility, and molecular weight of the components Poperelya, 1980: Obukhova andShumny, 2016). The use of buffer mixtures containing lactic (Bushuk and Zillman, 1978, Metakovsky et al, 1984: Branlard et al, 1990Utebayev et al, 2019;Watry et al, 2020), formic (Kozub et al, 2009), and acetic (Clements, 1988;Konarev et al, 2005) acids determines the total gliadin by electrophoresis on a polyacrylamide gel.…”

Section: Introductionmentioning

confidence: 99%

Allelic Composition of Gliadin-Coding Loci as a 'Portrait' in Spring Soft Wheat Selections of Russian and Kazakh Origins

UTEBAYEV¹,

DOLINNY²,

DASHKEVICH³

et al. 2022

SABRAOJBG

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The progressive study determined the gliadin 'profile' and genetic diversity based on allelic variations of gliadin-coding loci in spring soft wheat (Triticum aestivum L.) selection from Russian and Kazakh origins. A total of 139 samples obtained from a spring soft wheat collection consisting of 62 from Russia and 77 from Kazakhstan were studied. As a result of electrophoretic analysis in Russian wheat, the share of monomorphic gliadin cultivars was 63% and polymorphic was 37%. However, in the Kazakh wheat collection, the share of polymorphic gliadin cultivars was 26%. The alleles were mostly found among the Russian cultivars, i.e., Gli-А1f (46.9%), Gli-В1е (43.7%), Gli-В1b (30.8%), Gli-D1а (61.0%), Gli-А2l (17.0%), Gli-А2m (16.9%), Gli-В2o (16.1%), and Gli-D2q (19.8%). In wheat genotypes collected from Kazakhstan, the following alleles dominated, i.e., Gli-А1f (47.4%), Gli-В1е (72.0%), Gli-D1а (61.7%), Gli-А2l (25.3%), Gli-А2s (16.2%), Gli-В2r (25.7%), and Gli-D2a (40.3%). The study compiled the so-called 'ideal' electrophoretic spectrum of gliadin for several countries to visualize the 'portrait' of wheat, created based on common blocks of gliadin identified by the researchers at different times. It assumed that cultivars close to the 'ideal' spectrum in gliadin alleles should have a complex of economically valuable features. For example, the spectrum of Russian wheat consists of the blocks of components controlled by alleles, i.e., f, e, a, q, o, e. As for the Kazakh wheat, its 'ideal' spectrum (f, e, a, l, r, a) coincides with the spectrum of Russian wheat at the loci Gli-1, as Russian cultivars were often taken as parental genotypes by the Kazakhstan breeders.

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Genetics and genomics of wheat: Storage proteins, ecological plasticity, and immunity

Novoselskaya-Dragovich

2015

Russ J Genet

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Recent advances in genetics and genetic research methods made it possible to explain the large polymorphism observed among storage proteins of wheat grain (gliadins and glutenins), to determine their genetic control mechanism, and to develop a system for the identification of wheat genotypes on the basis of multiple allelism. This system has extremely high sensitivity and efficiency, which makes it possible to con duct studies to determine the purity and authenticity of wheat cultivars, the dynamics of alleles diversity in time and space, the phylogenetics, etc., through the use of an extensive database on the allelic composition of gliadin loci. An investigation of the molecular structure of genes controlling the synthesis of storage pro teins and their organization on chromosomes, as well as an analysis of wheat genome structure, revealed the molecular mechanisms of variability in the wheat genome and its reorganization in response to changes in environmental conditions and cultivation technologies. The multilevel genetic system of protection against pathogens and adverse environmental factors that developed in the course of wheat evolution continues to astound researchers' imagination with new resistance genes and novel types of antimicrobial peptides having been discovered and sequenced in recent years and the diversity of their structures and mechanisms of action in response to pathogens. An analysis of nucleotide sequences involved in wheat domestication, namely, those that define ecological plasticity, i. e. the type of plant development (Vrn genes), and those responsible for spikelet traits (Q genes), which ensured the successful cultivation of wheat by humans, revealed that the basis for these features are specific mutations.

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Gliadin Electrophoretic Analysis of the Genetic Integrity of Wheat (Triticum aestivum L.) Accessions After Frequent Seed Reproductions

Cited by 3 publications

References 9 publications

Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

Tetraploid and hexaploid wheat varieties reveal large differences in expression of alpha-gliadins from homoeologous Gli-2 loci

Allelic Composition of Gliadin-Coding Loci as a 'Portrait' in Spring Soft Wheat Selections of Russian and Kazakh Origins

Genetics and genomics of wheat: Storage proteins, ecological plasticity, and immunity

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