Glial and axonal regeneration following spinal cord injury

Shibuya, Sei; Yamamoto, Toshiyuki; Itano, Toshifumi

doi:10.4161/cam.3.1.7372

Cited by 22 publications

(22 citation statements)

References 107 publications

(97 reference statements)

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“…the clearance of cellular debris, genesis of the insulating myelin sheath around regenerating nerve fibers and synaptogenesis). 2,3 Therefore, a promising strategy to enhance regeneration is to reconstruct glial cell circuitry in lesion sites via the implantation of organized glial cell constructs. Currently, a major strategy to achieve this goal in neural tissue engineering studies utilizes highly aligned electrospun nanofibers for the attachment/alignment of individual glial cell populations, e.g.…”

Section: Introductionmentioning

confidence: 99%

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

Weightman

Jenkins

Pickard

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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. (2014). Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury. Nanomedicine: Nanotechnology, Biology and Medicine, 10(2), Page 2 of 14 Graphical AbstractSchematic diagram depicting the production and culture of complex 3D multi-cellular constructs using a sequential cell seeding procedure. Astrocytes (1) were seeded on aligned nanofiber-collagen hydrogel constructs followed by addition of oligodendrocyte precursor cells (OPCs; (2)) after astrocyte alignment. The multicellularity of the constructs containing OPCs, mature oligodendrocytes, type I and type II astrocytes is shown in (3).

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Section: Introductionmentioning

confidence: 99%

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

Weightman

Jenkins

Pickard

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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“…It has been previously observed that the absence of astrocytes in both white and gray matter results in unsuccessful axon regeneration. The presence of immature fibrous astrocytes in the white matter and reactive protoplasmic astrocytes in the gray matter of the spinal cord within the injury site in the early post-injury phase are necessary for axon regeneration (Shibuya, Yamamoto & Itano, 2009;Kikukawa et al, 1998;Inoue, Kawaguchi, & Kurisu, 1998). Protoplasmic astrocyte enumeration in the IKVAV-peptide group demonstrated an increased number compared to all groups including the normal nonsurgical group.…”

Section: Discussionmentioning

confidence: 94%

IKVAV-Containing Cell Membrane Penetrating Peptide Treatment Induces Changes in Cellular Morphology after Spinal Cord Injury

Kazemi¹,

Baltzer²,

Mansouri³

et al. 2016

MAS

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A cell membrane spanning peptide was used to increase the concentration of the IKVAV motif within damaged mouse spinal cord tissue. This peptide was injected directly to the lesion 24 hours after spinal cord compression injury. Because the membrane-spanning portion of the peptide adheres to tissue upon injection with a long half-life we hypothesized that the bioactive IKVAV sequence will provide a sustained regenerative signal at the sight of injury. Five different groups of mice were used and cellular morphology observations were undertaken using light and electron microscopy. Three surgical control groups: IKVAV, peptide and mannitol; one surgical treatment group: IKVAV-peptide; and one non-surgical control group: normal, were used in this experiment. In this study, treatment with IKVAV-peptide after SCI resulted in an increased number of protoplasmic astrocytes, large active motor neurons, and regeneration of muscle bundles followed by behavioral improvement. In this paper, we describe the cellular differences between all groups.

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“…Emergence and migration of reactive astrocytes have a prominent role in the repair of injured tissue and the restoration of motor function in the subacute phase of healing neuronal tissue before completion of the glial scar [35]. Loss of astrocyte activities during the cellular response to SCI can lead to gross functional deficits and failure of functional recovery and suggest that reactive astrocytes not only protect tissue but also preserve function after SCI [36,37]. …”

Section: Discussionmentioning

confidence: 99%

IKVAV-linked cell membrane-spanning peptide treatment induces neuronal reactivation following spinal cord injury

et al. 2015

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Spinal cord regeneration following treatment with a novel membrane-spanning peptide (MSP) expressing the isoleucine-lysine-valine-alanine-valine (IKVAV) epitope was assessed in Balb-c mice. After hemilaminectomy and compression injury, mice were treated with IKVAV, IKVAV-MSP, peptide or vehicle control. Functional improvement was assessed using modified Basso, Beattie, and Bresnahan Scale (mBBB) and spinal cord segments were studied histologically 28 days after injury. IKVAV-MSP group scores increased significantly compared with control groups after 4 weeks of observation (p < 0.05). The number of protoplasmic astrocytes, neurons and muscle bundle size in the IKVAV-MSP mice were significantly increased (p < 0.001; p < 0.05 and p < 0.007; respectively). This study demonstrates that it is possible to promote functional recovery after SCI using bioactive IKVAV presenting cell membrane-spanning peptides.

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Glial and axonal regeneration following spinal cord injury

Cited by 22 publications

References 107 publications

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury

IKVAV-Containing Cell Membrane Penetrating Peptide Treatment Induces Changes in Cellular Morphology after Spinal Cord Injury

IKVAV-linked cell membrane-spanning peptide treatment induces neuronal reactivation following spinal cord injury

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