Glioma cell line proliferation controlled by different chemical functional groups in vitro

Xu, Suju; Cui, Fuzhai; Yu, Xiaolong; Kong, Xiangdong

doi:10.1007/s11706-013-0195-7

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…This is supported by the contracted and 3D solid morphology of fibroblast cells on MPTMS-ATNs (Figure C,F), which is different from the flat morphology observed in APTMS-ATNs (Figure B,E). Previous studies have shown that mercapto functional groups are highly reactive and easily form new disulfide bonds with proteins through an interchange–reduction reaction. − Although mercapto group-modified silicon surfaces were shown to lead to fewer cancer cells in comparison with −COOH-, −NH 2 -, −OH-, and −CH 3 -modified substrates, the mechanism of cell attachment or detachment on these modified substrates has not been discussed. As shown by vinculin immunostaining, cells on our MPTMS-ATNs revealed a relatively 3D solid morphology and clear contours (Figure ) caused by the lack of focal adhesions (Figure I).…”

Section: Resultsmentioning

confidence: 99%

Investigation of the Interfacial Effects of Small Chemical-Modified TiO₂ Nanotubes on 3T3 Fibroblast Responses

Lin

Huang²,

Chen³

et al. 2014

ACS Appl. Mater. Interfaces

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In order to gain insight into how interfacial effects influence cell responses, chemically modified anodized TiO2 nanotubes (ATNs) were used to simultaneously investigate the effects of nanoscale substrate structure and angstrom-scale chemicals on cell morphological change and cell growth. Two small chemicals were used to modify the ATNs, namely, 3-aminopropyltrimethoxysilane (APTMS) and 3-mercaptopropyltrimethoxysilane (MPTMS), resulting in APTMS-modified ATNs (APTMS-ATNs) and MPTMS-modified ATNs (MPTMS-ATNs), respectively. In our in vitro observation of NIH/3T3 fibroblasts, cells thrived on both unmodified and modified ATNs. Quantitative analyses of cell numbers exhibited that APTMS-ATNs effectively facilitated cell proliferation and directed cell orientation owing to full cell-substrate contact caused by positively charged amino groups (-NH3(+)) on the surface. In addition, scanning electron microscopy and fluorescence images showed different cell morphologies on APTMS-ATNs and MPTMS-ATNs. APTMS-ATNs resulted in flat spreading of fibroblasts, while MPTMS-ATNs resulted in fibroblasts with a three-dimensional solid shape and clear contours. The results indicate that the synergistic effects of nanotube surface topology and small chemical modification and, to a lesser extent, surface hydrophilicity, alter the interfacial interactions between cells and substrates, significantly affecting cell morphology, attachment, and growth. Using ATNs with different interfacial effects from various small chemicals, orientation of cells into various patterns can be achieved and investigation of cell fates, such as proliferation or stem cell differentiation, can be performed for future advanced medical or biological applications.

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Section: Resultsmentioning

confidence: 99%

Investigation of the Interfacial Effects of Small Chemical-Modified TiO₂ Nanotubes on 3T3 Fibroblast Responses

Lin

Huang²,

Chen³

et al. 2014

ACS Appl. Mater. Interfaces

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“…To determine the sole effect of chemical groups on cellular behavior, self-assembled monolayers (SAMs) of thiols or silanes have been typically employed on fl at surfaces. For studies using a single type of functionalized alkane thiols, general trends in the cell number has been observed regardless of different cell types, where, for example the growth of bovine aortic endothelial cells (BAEC) [ 76 ] increased in the order of OH < COOCH 3 < CH 3 < COOH, glioma cell line C6 [ 77 ] generally increased in the order of SH < CH 3 ≤ OH < NH 2 < COOH, and MCF-7 cancer cells [ 78 ] increased in the order of SH ≤ OH ≤ CH 3 < NH 2 ≤ COOH. These studies have been conducted with the presence of serum, where some studies have highlighted the correlation between chemical functional groups and the level of elutability of adsorbed albumin and adsorption of cell adhesive proteins such as fi bronectin [ 76 , 79 , 80 ].…”

Section: Chemical Cuesmentioning

confidence: 99%

Guided Cellular Responses by Surface Cues for Nanomedicine Applications

Ogaki

Andersen

Foss

2016

Advances in Delivery Science and Technology

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Using surface cues to guide and ultimately control cellular responses is of paramount importance in numerous biomedical applications. Since cells react on feature sizes both on the micro and nanometer scale, these length scales are crucial parameters when designing new materials for applications in medicine e.g. for tissue engineering and drug delivery. Thus, variation of the features at the nanometer length scale is an integral part of nanomedicine research and development. In this chapter the interaction between biological systems and artifi cial materials will be addressed in general with focus on simplifi ed model systems where only one or a few parameters are varied at two-dimensional (2D) surfaces. At fi rst biomolecular adsorption/immobilization on surfaces will be addressed followed by a discussion of approaches to synthesize functionalized surfaces and the infl uence of such surfaces on cellular response. Some of the key parameters, which will be discussed in more detail, are topography, chemistry, and elastic modulus of the substrate. Even though there is a vast amount of published data it will become clear that there is still not a detailed understanding of the infl uence of these parameters on biosystems at the cellular level. Obviously, the degree of complexity increases when several of the surface cues are combined. The challenges in understanding the cellular responses in detail in real systems with the simultaneous variation of multiple parameters leads to the introduction of the fi eld of high throughput screening of biomaterials.

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Regulation effect of osteoblasts towards osteocytes by silk fibroin encapsulation

et al. 2022

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Glioma cell line proliferation controlled by different chemical functional groups in vitro

Cited by 3 publications

References 26 publications

Investigation of the Interfacial Effects of Small Chemical-Modified TiO₂ Nanotubes on 3T3 Fibroblast Responses

Investigation of the Interfacial Effects of Small Chemical-Modified TiO₂ Nanotubes on 3T3 Fibroblast Responses

Guided Cellular Responses by Surface Cues for Nanomedicine Applications

Regulation effect of osteoblasts towards osteocytes by silk fibroin encapsulation

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Glioma cell line proliferation controlled by different chemical functional groups in vitro

Cited by 3 publications

References 26 publications

Investigation of the Interfacial Effects of Small Chemical-Modified TiO2 Nanotubes on 3T3 Fibroblast Responses

Investigation of the Interfacial Effects of Small Chemical-Modified TiO2 Nanotubes on 3T3 Fibroblast Responses

Guided Cellular Responses by Surface Cues for Nanomedicine Applications

Regulation effect of osteoblasts towards osteocytes by silk fibroin encapsulation

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Product

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Investigation of the Interfacial Effects of Small Chemical-Modified TiO₂ Nanotubes on 3T3 Fibroblast Responses

Investigation of the Interfacial Effects of Small Chemical-Modified TiO₂ Nanotubes on 3T3 Fibroblast Responses