Glioma‐Targeted Delivery of a Theranostic Liposome Integrated with Quantum Dots, Superparamagnetic Iron Oxide, and Cilengitide for Dual‐Imaging Guiding Cancer Surgery

Abstract

Herein, a theranostic liposome (QSC-Lip) integrated with superparamagnetic iron oxide nanoparticles (SPIONs) and quantum dots (QDs) and cilengitide (CGT) into one platform is constructed to target glioma under magnetic targeting (MT) for guiding surgical resection of glioma. Transmission electron microscopy and X-ray photoelectron spectroscopy confirm the complete coencapsulation of SPIONs and QDs in liposome. Besides, CGT is also effectively encapsulated into the liposome with an encapsulation efficiency of ∼88.9%. QSC-Lip exhibits a diameter of 100 ± 1.24 nm, zeta potential of -17.10 ± 0.11 mV, and good stability in several mediums. Moreover, each cargo shows a biphasic release pattern from QSC-Lip, a rapid initial release within initial 10 h followed by a sustained release. Cellular uptake of QSC-Lip is significantly enhanced by C cells under MT. In vivo dual-imaging studies show that QSC-Lip not only produces an obvious negative-contrast enhancement effect on glioma by magnetic resonance imaging but also makes tumor emitting fluorescence under MT. The dual-imaging of QSC-Lip guides the accurate resection of glioma by surgery. Besides, CGT is also specifically distributed to glioma after administration of QSC-Lip under MT, resulting in an effective inhibition of tumors. The integrated liposome may be a potential carrier for theranostics of tumor.

Immunoengineering in glioblastoma imaging and therapy

“…Xu et al () (X. Wang et al, ) designed a theranostic liposome loaded with cilengitide, a cyclic Arg‐Gly‐Asp peptide with potential anticancer activity, combined SPIONs and QDs into a single platform for magnetic targeting of glioma targeting. In vivo, following magnetic field exposure, the nanoplatform was able to accumulate at tumor sites and inhibit tumor growth.…”

“…They can be functionalized and hybridized with different moieties for tumortargeted real-time imaging in the diagnosis and surgical resection of glioma (Zayed, AbdElhamid, Freag, & Elzoghby, 2019). Xu et al (2018) (X. Wang et al, 2012) designed a theranostic liposome loaded with cilengitide, a cyclic Arg-Gly-Asp peptide with potential anticancer activity, combined SPIONs and QDs into a single platform for magnetic targeting of glioma targeting.…”

Surface Modification of Iron Oxide-Based Magnetic Nanoparticles for Cerebral Theranostics: Application and Prospection

“…Different from INOPs in the core, INOPs were produced in the hydrophobic region of phospholipid bilayer with quantum dots (QDs), a new optical imaging method for molecular tracing and biomedical diagnostics, and cilengitide (CGT), inhibiting overexpress of integrin receptors. Using ultrasound-targeted microbubble destruction for delivery, the median survival of glioma rat was significantly extended to 59.6d compared with the control’s (20d) [ 139 ].…”

Hybrid Vesicular Drug Delivery Systems for Cancer Therapeutics

“…Cilengitide (an antiangiogenic drug) was encapsulated in the core using a film rehydration method. QDs (CdSe/ZnS) and SPIONs were loaded into liposomal bilayers ( Figure ) . The results showed that the designed dual imaging particles not only provided a negative‐contrast enhancement effect on the glioma through magnetic resonance imaging but also produced tumor emitting fluorescence under magnetic targeting.…”