Global Chromatin Accessibility Profiling Analysis Reveals a Chronic Activation State in Aged Muscle Stem Cells

Dong, A.; Liu, Jing; Lin, Kangning; So, Wai-Kin; Hu, Shenyuan; Cheung, Tom H.

doi:10.2139/ssrn.4003712

Cited by 1 publication

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“…In addition, we identified chromatin loops within the Pax7 TAD involving four interconnected REs (R1, R2, R3, and R4), including promoter, enhancers, and boundary elements. Notably, during the revision of our manuscript, Dong et al (65) reported the identification of an intronic and a distal enhancer of Pax7 in SCs using chromatin accessibility profiling by ATAC-seq and proved their regulatory functions. These two enhancers happen to be our R2 and R3.…”

Section: Discussionmentioning

confidence: 82%

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Multiscale 3D genome reorganization during skeletal muscle stem cell lineage progression and aging

Zhao

Ding

et al. 2023

Sci. Adv.

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Little is known about three-dimensional (3D) genome organization in skeletal muscle stem cells [also called satellite cells (SCs)]. Here, we comprehensively map the 3D genome topology reorganization during mouse SC lineage progression. Specifically, rewiring at the compartment level is most pronounced when SCs become activated. Marked loss in topologically associating domain (TAD) border insulation and chromatin looping also occurs during early activation process. Meanwhile, TADs can form TAD clusters and super-enhancer–containing TAD clusters orchestrate stage-specific gene expression. Furthermore, we uncover that transcription factor PAX7 is pivotal in enhancer-promoter (E-P) loop formation. We also identify cis-regulatory elements that are crucial for local chromatin organization at Pax7 locus and Pax7 expression. Lastly, we unveil that geriatric SC displays a prominent gain in long-range contacts and loss of TAD border insulation. Together, our results uncover that 3D chromatin extensively reorganizes at multiple architectural levels and underpins the transcriptome remodeling during SC lineage development and SC aging.

show abstract

Section: Discussionmentioning

confidence: 82%

“…Notably, during the revision of our manuscript, Dong et al. ( 65 ) reported the identification of an intronic and a distal enhancer of Pax7 in SCs using chromatin accessibility profiling by ATAC-seq and proved their regulatory functions. These two enhancers happen to be our R2 and R3.…”

Section: Discussionmentioning

confidence: 84%

Multiscale 3D genome reorganization during skeletal muscle stem cell lineage progression and aging

Zhao

Ding

et al. 2023

Sci. Adv.

View full text Add to dashboard Cite

show abstract

Global Chromatin Accessibility Profiling Analysis Reveals a Chronic Activation State in Aged Muscle Stem Cells

Cited by 1 publication

References 0 publications

Multiscale 3D genome reorganization during skeletal muscle stem cell lineage progression and aging

Multiscale 3D genome reorganization during skeletal muscle stem cell lineage progression and aging

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