2005
DOI: 10.1002/bit.20579
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Global expression profiling of Bacillus subtilis cells during industrial‐close fed‐batch fermentations with different nitrogen sources

Abstract: A detailed gene expression analysis of industrial-close Bacillus subtilis fed-batch fermentation processes with casamino acids as the only nitrogen source and with a reduced casamino acid concentration but supplemented by ammonia was carried out. Although glutamine and arginine are supposed to be the preferred nitrogen sources of B. subtilis, we demonstrate that a combined feeding of ammonia and casamino acids supports cell growth under fed-batch fermentation conditions. The transcriptome and proteome analyses… Show more

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Cited by 40 publications
(36 citation statements)
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“…The quality of RNA preparations was controlled using the Agilent 2100 Bioanalyzer according to the manufacturer's instructions (Agilent Technologies, Waldbronn, Germany). Generation of the Cy3/Cy5-labeled cDNAs and hybridization to wholegenome DNA microarrays (Eurogentec, Cologne, Germany) containing DNA fragments originating from 4,022 B. subtilis genes as duplicate spots were performed as described by Jürgen et al (35). The slides were scanned with a ScanArray Express scanner (PerkinElmer Life and Analytical Sciences, Monza, Italy), and signal intensities of the individual spots were quantified with the ScanArray Express image analysis software (PerkinElmer Life and Analytical Sciences).…”
Section: Methodsmentioning
confidence: 99%
“…The quality of RNA preparations was controlled using the Agilent 2100 Bioanalyzer according to the manufacturer's instructions (Agilent Technologies, Waldbronn, Germany). Generation of the Cy3/Cy5-labeled cDNAs and hybridization to wholegenome DNA microarrays (Eurogentec, Cologne, Germany) containing DNA fragments originating from 4,022 B. subtilis genes as duplicate spots were performed as described by Jürgen et al (35). The slides were scanned with a ScanArray Express scanner (PerkinElmer Life and Analytical Sciences, Monza, Italy), and signal intensities of the individual spots were quantified with the ScanArray Express image analysis software (PerkinElmer Life and Analytical Sciences).…”
Section: Methodsmentioning
confidence: 99%
“…Total RNA was isolated by the acid phenol method as described previously (19). Generation of fluorescence-labeled cDNA and hybridization with B. subtilis whole-genome microarrays (Eurogentec) was performed as described previously (11). Two independent hybridization experiments were performed using RNAs from two independent experiments.…”
Section: Bacterialmentioning
confidence: 99%
“…The RNA samples obtained from three independent cultivations were used for independent cDNA synthesis and DNA microarray hybridization. The generation of the Cy3/Cy5-labeled cDNAs and hybridization to B. subtilis whole-genome DNA microarrays (Eurogentec) were performed as described previously (32). The slides were scanned with a ScanArray Express scanner (PerkinElmer).…”
mentioning
confidence: 99%