Global gene expression analysis of knockdown Triosephosphate isomerase (TPI) gene in human gastric cancer cell line MGC-803

Ouyang, Ping; Lin, Bode; Du, Jinlin; Pan, Haiyan; Yu, Haibing; He, Rongwei; Huang, Zhigang

doi:10.1016/j.gene.2018.01.014

Cited by 6 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, FoxOs supported the metabolic requirements of normal and tumor cells via the PI3K signaling pathway, which was reported to interact with the cAMP signaling pathway in a number of physiological processes (41). In addition, systemic lupus erythematosus was the most significant pathway response to ENO1 inhibition, and this result was similar to that in our previous study on TPI silencing (22). As previously discussed, although no definitive evidence has suggested the involvement of systemic lupus erythematosus in the pathogenesis of GC, there may be an association between the two since certain sporadic patients were affected by GC and systemic lupus erythematosus simultaneously (22,42,43).…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803

et al. 2019

Self Cite

View full text Add to dashboard Cite

Gastric cancer (GC) is the third leading cause of cancer-associated mortality. In a previous study, we identified that α-enolase (ENO1) promoted cell migration in GC, but the underlying molecular mechanisms remain to be fully elucidated. In the present study, small interfering RNAs were identified to interfere with ENO1 expression. The cDNA expression profiling was performed using an Affymetrix mRNA array platform to identify genes that may be associated with ENO1 in human GC cell line MGC-803. The differentially expressed genes (DEGs) were identified using the reverse transcription-quantitative polymerase chain reaction, followed by a series of bioinformatic analyses. As a result, there were 448 DEGs, among which 183 (40.85%) were downregulated. The most significant functional terms for the DEGs were the nuclear lumen for cell components (P=2.83×10−4), transcription for biological processes (P=3.7×10−7) and transcription factor activity for molecular functions (P=1.16×104). In total, six significant pathways were enriched, including the most common cancer-associated forkhead box O signaling pathway (P=0.0077), microRNAs in cancer (P=0.0183) and the cAMP signaling pathway (P=0.0415). Furthermore, a network analysis identified three hub genes (HUWE1, PPP1CB and HSPA4), which were all involved in tumor metastasis. Taken together, the DEGs, significant pathways and hub genes identified in the present study shed some light on the molecular mechanisms of ENO1 involved in the pathogenesis of GC.

show abstract

Section: Discussionsupporting

confidence: 90%

“…The human GC cell line MGC-803 (Sun Yat-sen University Cell Library, Guangdong, China) was cultured as described previously (22). The small fragment small interfering RNA (siRNA) against ENO1 and the scrambled (control) siRNA were synthesized by Beijing Oligobio (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, in recent years, the molecular mechanisms of the relationship between TPI and cancer progression have been started to be investigated. The results of the studies provide strong evidence that TPI enzyme may be involved in tumor progression, drug resistance, invasion and metastasis …”

Section: The Role Of Triosephosphate Isomerase In Cancermentioning

confidence: 92%

“…According to these results, it is suggested that TPI level may effect on cancer cells behavior in gastric cancer, and may be considered a potential tumor marker and a new target in gastric cancer treatment . In order to elucidate the mechanism of the effect of TPI on gastric cancer, Ouyang et al . examined differentiated gene expression in TPI knockdown cell lines.…”

Section: The Role Of Triosephosphate Isomerase In Cancermentioning

confidence: 99%

See 1 more Smart Citation

Therapeutic Targeting of Cancer Metabolism with Triosephosphate Isomerase

Pekel

Arı

2020

Chemistry & Biodiversity

View full text Add to dashboard Cite

The increase in glycolytic flux in cancer, known as aerobic glycolysis, is one of the most important hallmarks of cancer. Therefore, glycolytic enzymes have importance in understanding the molecular mechanism of cancer progression. Triosephosphate isomerase (TPI) is one of the key glycolytic enzymes. Furthermore, it takes a part in gluconeogenesis, pentose phosphate pathway and fatty acid biosynthesis. To date, it has been shown altered levels of TPI in various cancer types, especially in metastatic phenotype. According to other studies, TPI might be considered as a potential therapeutic target and a cancer-related biomarker in different types of cancer. However, its function in tumor formation and development has not been fully understood. Here, we reviewed the relationship between TPI and cancer for the first time

show abstract

Prognostic value of small nucleolar RNAs (snoRNAs) for colon adenocarcinoma based on RNA sequencing data

Huang

Liang

Deng

et al. 2020

Pathology - Research and Practice

View full text Add to dashboard Cite

Global gene expression analysis of knockdown Triosephosphate isomerase (TPI) gene in human gastric cancer cell line MGC-803

Cited by 6 publications

References 36 publications

Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803

Gene expression profile analysis of ENO1 knockdown in gastric cancer cell line MGC‑803

Therapeutic Targeting of Cancer Metabolism with Triosephosphate Isomerase

Prognostic value of small nucleolar RNAs (snoRNAs) for colon adenocarcinoma based on RNA sequencing data

Contact Info

Product

Resources

About