Global Lysine Acetylation and 2-Hydroxyisobutyrylation Profiling Reveals the Metabolism Conversion Mechanism in Giardia lamblia

Zhu, Wenhe; Jiang, Xiaoming; Sun, Hongyu; Li, Yawei; Shi, Wen‐Yan; Zheng, Meiyu; Liu, Di; Ai-xin, MA; Feng, Xiao

doi:10.1074/mcp.ra120.002353

Cited by 11 publications

(9 citation statements)

References 57 publications

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“…That meaning that the Khib of ENO1 play an important role of regulating of cellular glycolysis and cell growth (48). Previous studies have shown that phosphoglycerate kinase (pgK) of metabolism enzymes play an important role of regulating the glycolytic process and glucose metabolism (68)(69)(70). Enhancing pgK modification of ENO1K343 causes the decreasing of enzyme activities of ENO1 (61).…”

Section: Post-translational Modifications Of Eno1 Play An Important Role In the Function Change Of Eno1mentioning

confidence: 99%

Enolase 1, a Moonlighting Protein, as a Potential Target for Cancer Treatment

Qiao¹,

Wu²,

Chen³

et al. 2021

Int. J. Biol. Sci.

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Enolase 1 (ENO1) is a moonlighting protein, function as a glycolysis enzyme, a plasminogen receptor and a DNA binding protein. ENO1 play an important role in the process of cancer development. The transcription, translation, post-translational modifying activities and the immunoregulatory role of ENO1 at the cancer development is receiving increasing attention. Some function model studies have shown that ENO1 is a potential target for cancer treatment. In this review, we provide a comprehensive overview of the characterization, function, related transduction cascades of ENO1 and its roles in the pathophysiology of cancers, which is a consequence of ENO1 signaling dysregulation. And the development of novels anticancer agents that targets ENO1 may provide a more attractive option for the treatment of cancers. The data of sarcoma and functional cancer models indicates that ENO1 may become a new potential target for anticancer therapy.

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Section: Post-translational Modifications Of Eno1 Play An Important Role In the Function Change Of Eno1mentioning

confidence: 99%

Enolase 1, a Moonlighting Protein, as a Potential Target for Cancer Treatment

Qiao¹,

Wu²,

Chen³

et al. 2021

Int. J. Biol. Sci.

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“…Several post-translational modifications (PTMs) of proteins are reportedly involved in Giardia encystation, namely deacetylation [ 58 ] and phosphorylation [ 59 ]. Consequently, several proteomic studies have aimed to characterise the PTMs of proteins in G. duodenalis [ 4 , 11 , 15 , 60 , 61 , 62 ], such as the role of G. duodenalis DHHC proteins in protein S-palmitoylation during Giardia encystation [ 61 ]. Specifically, Merino et al (2014) reported that nine DDHC proteins were identified in trophozoites and encysting cells of G. duodenalis , and concluded that the presence of DDHC proteins in the encysting parasites indicates that the protein S-palmitoylation is maintained and involved in cell signalling, protein-sorting, and protein exporting throughout encystation.…”

Section: Applications Of Proteomicsmentioning

confidence: 99%

“…Another recent study, by Zhu et al (2021), that utilised a global approach in investigating metabolic conversion mechanisms of G. duodenalis under stress revealed a total of 2999 lysine acetylation (Kac) sites on 956 proteins and 8877 2-hydroxyisobutyrylation (Khib) sites on 1546 proteins when G. duodenalis was under sugar starvation [ 62 ]. The authors noted a temporal reduction in both Kac and Khib proteins when G. duodenalis was cultured under sugar starvation for 72 h, indicating their involvement in energy conversion metabolism.…”

Section: Applications Of Proteomicsmentioning

confidence: 99%

“…The authors noted a temporal reduction in both Kac and Khib proteins when G. duodenalis was cultured under sugar starvation for 72 h, indicating their involvement in energy conversion metabolism. They concluded that the correlation of acetylation and 2-hydroxyisobutyrylation expressed proteins linked to amino acid metabolism, suggesting that Giardia ’s regulatory mechanism involves dynamic changes in acetylation modification to supply energy in the absence of glucose [ 62 ].…”

Section: Applications Of Proteomicsmentioning

confidence: 99%

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Application of Proteomics to the Study of the Therapeutics and Pathogenicity of Giardia duodenalis

et al. 2022

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Giardia duodenalis remains a neglected tropical disease. A key feature of the sustained transmission of Giardia is the ability to form environmentally resistant cysts. For the last 38 years, proteomics has been utilised to study various aspects of the parasite across different life cycle stages. Thirty-one articles have been published in PubMed from 2012 to 2022 related to the proteomics of G. duodenalis. Currently, mass spectrometry with LC-MS/MS and MALDI-TOF/TOF has been commonly utilised in proteomic analyses of Giardia, which enables researchers to determine potential candidates for diagnostic biomarkers as well as vaccine and drug targets, in addition to allowing them to investigate the virulence of giardiasis, the pathogenicity mechanisms of G. duodenalis, and the post-translational modifications of Giardia proteins throughout encystation. Over the last decade, valuable information from proteomics analyses of G. duodenalis has been discovered in terms of the pathogenesis and virulence of Giardia, which may provide guidance for the development of better means with which to prevent and reduce the impacts of giardiasis. Nonetheless, there is room for improving proteomics analyses of G. duodenalis, since genomic sequences for additional assemblages of Giardia have uncovered previously unknown proteins associated with the Giardia proteome. Therefore, this paper aims to review the applications of proteomics for the characterisation of G. duodenalis pathogenicity and the discovery of novel vaccine as well as drug targets, in addition to proposing some general directions for future Giardia proteomic research.

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“…Khib adds a large 2-hydroxyisobutyryl group to the lysine residue, which increases the size of the lysine side chain, abolishes the positive charge, and introduces a hydroxyl group that enables the modified lysine to form hydrogen bonds with other molecules [1]. As a result, Khib can influence various biological processes, such as the regulation of gene expression, energy metabolism, protein translation, and secondary metabolism [1,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Quantitative multiplexed proteomics analysis reveals reshaping of the lysine 2-hydroxyisobutyrylome in Fusarium graminearum by tebuconazole

Zhao

Zhang

et al. 2022

BMC Genomics

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Backgrounds Lysine 2-hydroxyisobutyrylation (Khib) is a newly discovered posttranslational modification (PTM) and has been identified in several prokaryotic and eukaryotic organisms. Fusarium graminearum, a major pathogen of Fusarium head blight (FHB) in cereal crops, can cause considerable yield loss and produce various mycotoxins that threaten human health. The application of chemical fungicides such as tebuconazole (TEC) remains the major method to control this pathogen. However, the distribution of Khib in F. graminearum and whether Khib is remodified in response to fungicide stress remain unknown. Results Here, we carried out a proteome-wide analysis of Khib in F. graminearum, identifying the reshaping of the lysine 2-hydroxyisobutyrylome by tebuconazole, using the most recently developed high-resolution LC–MS/MS technique in combination with high-specific affinity enrichment. Specifically, 3501 Khib sites on 1049 proteins were identified, and 1083 Khib sites on 556 modified proteins normalized to the total protein content were changed significantly after TEC treatment. Bioinformatics analysis showed that Khib proteins are involved in a wide range of biological processes and may be involved in virulence and deoxynivalenol (DON) production, as well as sterol biosynthesis, in F. graminearum. Conclusions Here, we provided a wealth of resources for further study of the roles of Khib in the fungicide resistance of F. graminearum. The results enhanced our understanding of this PTM in filamentous ascomycete fungi and provided insight into the remodification of Khib sites during azole fungicide challenge in F. graminearum.

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Global Lysine Acetylation and 2-Hydroxyisobutyrylation Profiling Reveals the Metabolism Conversion Mechanism in Giardia lamblia

Abstract: A metabolism conversion occurs in Giardia lamblia.• Glucose is not the only source of energy for G. lamblia.• Kac and Khib provides insight into metabolism conversion mechanism in G. lamblia.• Findings provide useful information of the evolutionary rule from prokaryote to eukaryote.

Cited by 11 publications

References 57 publications

Enolase 1, a Moonlighting Protein, as a Potential Target for Cancer Treatment

Enolase 1, a Moonlighting Protein, as a Potential Target for Cancer Treatment

Application of Proteomics to the Study of the Therapeutics and Pathogenicity of Giardia duodenalis

Quantitative multiplexed proteomics analysis reveals reshaping of the lysine 2-hydroxyisobutyrylome in Fusarium graminearum by tebuconazole

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