Global Model for Optimizing Crossflow Microfiltration and Ultrafiltration Processes: A New Predictive and Design Tool

Baruah, Gautam Lal; Venkiteshwaran, Adith; Belfort, Georges

doi:10.1021/bp050184r

Cited by 24 publications

(8 citation statements)

References 40 publications

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“…Particle trajectory analysis is an exception to this rule [165,[171][172][173][174][175][176][177]. Another exception is the global model and algorithm that predicts the performance of crossflow during MF and UF in the laminar flow regime [178]. A typical algorithm is shown in Fig.…”

Section: Quantitative Analysis and Membrane Performance Comparisonmentioning

confidence: 99%

“…This model and algorithm is an invaluable technique to rapidly design new or optimize existing MF and UF processes separately or in combination in both pressure-dependent and pressure-independent regimes. Its predictive capabilities for variations in solution conditions (pH and ionic strength), membrane properties (mean pore size and permeation flux) and fluid mechanical conditions (shear rate) are demonstrated with details of the model by Baruah et al (2005) [178].…”

Section: Quantitative Analysis and Membrane Performance Comparisonmentioning

confidence: 99%

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Critical aspects of RO desalination: A combination strategy

et al. 2017

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Due to a significant drop in the energy needed for RO desalination from 12 kWh/m 3 to ~2 kWh/m 3 over the past 20 years, which is close to 1 kWh/m 3 , the theoretical minimum for recovering salt-free water from seawater, the focus here is on the critical aspects that offer opportunities to further reduce costs. These include pre-and post-treatment; and analysis and optimization of the performance of RO systems, such as selectivity, capacity, and flux decline. Flux decline includes concentration polarization and fouling from inorganic, organic, biological constituents. We call these the "three legs' that undergird all membrane processes. The approach here is quantitative and includes detailed fluid mechanics and associated mass transfer of RO systems for optimizing performance.

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Section: Quantitative Analysis and Membrane Performance Comparisonmentioning

confidence: 99%

Section: Quantitative Analysis and Membrane Performance Comparisonmentioning

confidence: 99%

Critical aspects of RO desalination: A combination strategy

et al. 2017

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“…1 As for any unit operation, it is essential to have the ability to understand the process behavior at manufacturing scale at the stages of process development, design and optimization, as well as validation and trouble shooting. 2 This is especially true under the pressure to accelerate process development to commercialize therapeutics from more concentrated cell culture suspensions and fermentation broths.…”

Section: Introductionmentioning

confidence: 99%

“…2 This is especially true under the pressure to accelerate process development to commercialize therapeutics from more concentrated cell culture suspensions and fermentation broths. 1 Theoretical filtration models alone still cannot accurately predict the complex interactions in filtration operations. For complex feed material such as E. coli lysate containing antibody fragment, practical experiments are necessary to gain sufficient information for process-scale prediction.…”

Section: Introductionmentioning

confidence: 99%

Mimic of a large‐scale diafiltration process by using ultra scale‐down rotating disc filter

Aucamp

Gerontas

et al. 2009

Biotechnology Progress

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Ultra scale-down (USD) approach is a powerful tool to predict large-scale process performance by using very small amounts of material. In this article, we present a method to mimic flux and transmission performance in a labscale crossflow operation by an USD rotating disc filter (RDF). The Pellicon 2 labscale system used for evaluation of the mimic can readily be related to small pilot and industrial scale. Adopted from the pulsed sample injection technique by Ghosh and Cui (J Membr Sci. 2000;175:5-84), the RDF has been modified by building in inserts to allow the flexibility of the chamber volume, so that only 1.5 mL of processing material is required for each diafiltration experiment. The reported method enjoys the simplicity of dead-end mode operation with accurate control of operation conditions that can mimic well the crossflow operation in large scale. Wall shear rate correlations have been established for both the labscale cassette and the USD device, and a mimic has been developed by operating both scales under conditions with equivalent averaged shear rates. The studies using E. coli lysate show that the flux vs. transmembrane pressure profile follows a first-order model, and the transmission of antibody fragment (Fab') is independent of transmembrane pressure. Predicted flux and transmission data agreed well with the experimental results of a labscale diafiltration where the cassette resistance was considered.

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“…The isoelectric points of most of the HCP lie between pH 5 and 7 with molecular weights from 10 to about 100 kDa. Both experimental and theoretical studies (19−21) have examined the effects of pH and ionic strength on microfiltration of colloids (22−24) and ultrafiltration of proteins (25−29). However, neither pH nor ionic strength has been exploited to optimize MF of actual biological suspensions (30).…”

Section: Introductionmentioning

confidence: 99%

Optimized Removal of Soluble Host Cell Proteins for the Recovery of met-Human Growth Hormone Inclusion Bodies from Escherichia coli Cell Lysate Using Crossflow Microfiltration

Venkiteshwaran

Heider

Matosevic

et al. 2008

Biotechnol Progress

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Cross-flow membrane microfiltration was used under optimal conditions to recover met-growth hormone inclusion bodies (IBs) from Escherichia coli cell lysate by removal of the host-cell (bacterial) proteins (HCP) under minimal fouling conditions. This is the first step of a two-step process in which the goal was to isolate IBs at high yield from the HCP. These undesired soluble HCP were removed by passing them through the membrane while retaining the insolubles, including the aggregated IBs. Experiments were conducted at constant permeate flux with flat-sheet membranes of different pore sizes and chemistry, with feeds of varying pH and ionic strengths to determine the optimum combination for HCP removal. Diafiltration, the washing away of impurities with protein-free buffer, was then employed to ensure removal of the host cell proteins at the optimum conditions. About 90% removal of the HCP was obtained in about 5 diavolumes, maintaining high protein transmission and low membrane fouling.

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Global Model for Optimizing Crossflow Microfiltration and Ultrafiltration Processes: A New Predictive and Design Tool

Cited by 24 publications

References 40 publications

Critical aspects of RO desalination: A combination strategy

Critical aspects of RO desalination: A combination strategy

Mimic of a large‐scale diafiltration process by using ultra scale‐down rotating disc filter

Optimized Removal of Soluble Host Cell Proteins for the Recovery of met-Human Growth Hormone Inclusion Bodies from Escherichia coli Cell Lysate Using Crossflow Microfiltration

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