Global poly(A) mRNA expression profile measured in individual bovine oocytes and cleavage embryos

Biase, Fernando H.; Merighe, Giovana Krempel Fonseca; Biase, W. Karyna Freitas Santos; Martelli, Lúcia Regina; Meirelles, Flávio Vieira

doi:10.1017/s0967199407004455

Cited by 23 publications

(25 citation statements)

References 61 publications

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“…During the growth phase cattle oocytes synthesize a large amount of mRNA, this can be seen from the secondary follicle and is maintained up to the tertiary follicle (136,137) . In other words, in the bovine system transcription of mRNA begins to decline when the oocyte completes its growth and reactive meiosis II towards metaphase II (138) .…”

Section: Maternal Mrna In the Bovinementioning

confidence: 99%

“…Likewise, this process is mediated by the deadenylation of the extreme 3´UTR of the mRNA, through the action of proteins such as CPEB and eIF4G (139) . It has been shown that mature oocytes present mRNA with tails of polyadenin shorter than in those immature oocytes; however these mRNA with long tails of adenines, after fertilization in the stadium of 2 cells are deleted (137) . In cattle maternal mRNA storage during oogenesis is necessary to carry out a proper embryonic development (137) .…”

Section: Maternal Mrna In the Bovinementioning

confidence: 99%

“…It has been shown that mature oocytes present mRNA with tails of polyadenin shorter than in those immature oocytes; however these mRNA with long tails of adenines, after fertilization in the stadium of 2 cells are deleted (137) . In cattle maternal mRNA storage during oogenesis is necessary to carry out a proper embryonic development (137) . To date, few maternal factors have been described in cattle (Table 2), which include DICER, DROSHA, NMP2, MATER, ZAR1, NOBOX, ALPHA8, JY1 and DMT1.…”

Section: Maternal Mrna In the Bovinementioning

confidence: 99%

“…Durante la fase de crecimiento los ovocitos bovinos sintetizan una gran cantidad de RNAm, esto se observa a partir del folículo secundario y es mantenida hasta el folículo terciario (136,137) . En otras palabras, en el sistema bovino la transcripción de RNAm empieza a decaer cuando el ovocito termina su crecimiento y reactiva la meiosis II rumbo a metafase II (138) .…”

Section: Rnam Maternos En El Bovinounclassified

“…Así mismo este proceso es mediado por la desadenilación del extremo 3´UTR del RNAm, a través de la acción de proteínas como CPEB y eIF4G (139) . Se ha demostrado que los ovocitos maduros presentan RNAm con colas de poliadeninas más cortas que en aquellos ovocitos que son inmaduros; sin embargo estos RNAm con colas largas de adeninas, después de la fertilización en el estadio de 2 células son eliminados (137) . En el bovino el almacenaje de RNAm maternos durante la ovogénesis es necesario para que se lleve a cabo un correcto desarrollo embrionario (137) .…”

Section: Rnam Maternos En El Bovinounclassified

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos. RevisiónEffects of the maternal mRNA on the maturation of the oocyte and early embryonic development in mammals. Review M. Eduviges Burrola-Barraza a , Everardo González-Rodríguez a RESUMENObtener ovocitos madurados in vitro, que sean competentes para fertilizarse y originar un porcentaje superior al 50 % de blastocitos viables, es una de las principales metas que existen en el desarrollo in vitro de embriones bovinos. Es por ello necesario entender los procesos celulares, que desembocan en la maduración del ovocito durante la foliculogénesis y su subsecuente transición al embrión. La transición del ovocito al embrión es un proceso complejo que involucra la inactivación genómica del ovocito y la activación del genoma embrionario. Este proceso se da en bovinos en la etapa de 8 a 16 células y presenta una degradación selectiva de RNAm maternos, que fueron almacenados durante la ovogénesis y son la fuente para la codificación de proteínas en las etapas iníciales del desarrollo embrionario. La acción de los RNAm maternos es clave para que la activación del genoma embrionario se realice en tiempo y forma adecuados. Entender la participación de estos transcritos en la activación del genoma embrionario, es esencial para esclarecer los procesos celulares que fallan en los protocolos de maduración de ovocitos in vitro. Es por esto que el objetivo de esta revisión fue recopilar la información de los principales RNAm maternos que han sido identificados tanto en el modelo murino como el bovino, esto con el fin de integrar el conocimiento relacionado con los procesos genómicos que permiten el desarrollo del embrión en el bovino, y diseñar nuevas estrategias que permitan mejorar los protocolos de fertilización in vitro.PALABRAS CLAVE: Ovocito, Activación genoma embrionario, Bovino. ABSTRACTGet in vitro matured oocytes that could be competent for in vitro fertilization with a result of 50 % viable blastocysts, is one of the main problems in the in vitro development of bovine embryos. For these reason, is necessary to understand the cellular processes that lead to the maturation of the oocyte during folliculogenesis and subsequent transition to the embryo. The transition from oocyte to embryo is a complex process that involves genomic inactivation of the oocyte and embryonic genome activation. This process occurs in cattle in the stage of 8 to 16 cells where there are a selective degradation of maternal mRNA, which were stored during oogenesis and are the source of protein in the early stages of embryonic development. The action of maternal mRNA is a key for that embryonic genome activation takes place in form and time. Understanding the involvement of these transcripts in embryonic genome activation is essential to elucidate the cellular processes falling in oocyte maturation in vitro protocols. The aim of this review was to gather information from maternal genes that have been isolated both in the murine model and c...

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Section: Maternal Mrna In the Bovinementioning

confidence: 99%

Section: Maternal Mrna In the Bovinementioning

confidence: 99%

Section: Maternal Mrna In the Bovinementioning

confidence: 99%

Section: Rnam Maternos En El Bovinounclassified

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Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Burrola-Barraza¹,

González-Rodríguez

2015

Rev. Mex. Cienc. Pecu.

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The dynamics of gene products fluctuation during bovine pre‐hatching development

Gilbert

Scantland

Sylvestre

et al. 2009

Molecular Reproduction Devel

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Early embryonic development, spanning fertilization to blastocyst hatching, is a very dynamic developmental window that is characterized, especially in large mammals, by a period of transcriptional incompetence that ends during the maternal to embryonic transition (MET). Prior to the MET, the first cell cycles are supported by stored RNA and proteins pools accumulated during oogenesis. Therefore, RNA and protein content are different between developmental stages. It is also known that the stability of the stored mRNA and the mechanisms for translation recruitment are partly controlled by the length of the poly(A) tail. To date, little is known about RNA and protein content fluctuations during the pre-hatching period. In this report we present measurements of total RNA, mRNA, poly(A) bearing mRNA and protein contents, as well as estimations of the proportions of both mRNA fractions to total RNA contents within these developmental stages. We found that while the ontogenic profiles of the different transcript contents were expected, their amounts were considerably lower than the reported values. Additionally, low 28S rRNA abundance and a tendency for diminishing protein content prior to the MET, suggest a limited potential for ribosomal turnover and translation. We consider the overall fluctuations in RNA and protein contents to be reference points that are essential for downstream interpretation of gene expression data across stages whether it be through candidates or high throughput approaches.

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HOX genes are expressed in bovine and mouse oocytes and early embryos

Paul

Bridoux

Rezsöhazy

et al. 2011

Molecular Reproduction Devel

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HOX proteins are transcription factors that play a major role in patterning the body axis of vertebrates from the gastrulation stage. While nothing has been reported so far about their roles at earlier stages, there is evidence that some HOX genes are expressed before gastrulation. The objective of this work was to study the pattern of expression of several HOX genes during oocyte maturation and early embryonic development up to the blastocyst stage. Using nested PCR, HOXD1, HOXA3, HOXD4, HOXB7, HOXB9, and HOXC9 transcripts were detected in bovine oocytes and early embryos at various frequencies depending on the stage of development. Quantitative PCR was performed on bovine oocytes and early embryos: relative expression of HOXD1, HOXA3, and HOXC9 decreased sharply after the 5-8 cell stage. HOXB9 relative expression increased between the oocyte and the morula stage. All transcripts seemed to be of maternal origin before the maternal to embryonic transition, as demonstrated by blocking transcription with α-amanitin. Reverse transcription was performed with either hexamers or oligo-dT, allowing for the determination that HOXC9 transcripts were slightly deadenylated during oocyte maturation; HOXD1, HOXA3, and HOXB9 transcripts were not, indicating that they could be translated. Hoxd1, Hoxa3, Hoxb9, and Hoxc9 expression was also detected in mouse oocytes and early embryos. A similar pattern of expression was found in the two species. In conclusion, mammalian HOX genes might be implicated in the control of oocyte maturation, the maternal-to-embryonic transition or the first steps of embryo differentiation.

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Global poly(A) mRNA expression profile measured in individual bovine oocytes and cleavage embryos

Cited by 23 publications

References 61 publications

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

Efectos de los RNAm maternos sobre la maduración del ovocito y el desarrollo embrionario temprano en mamíferos

The dynamics of gene products fluctuation during bovine pre‐hatching development

HOX genes are expressed in bovine and mouse oocytes and early embryos

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