Global population variability in Promega PowerPlex CS7, D6S1043, and Penta B STRs

Kind, S.S.; Fernandez-Formoso, L.; Gelabert-Besada, Miguel; Carracedo, Ángel; Lareu, M.V.

doi:10.1007/s00414-013-0888-6

Cited by 11 publications

(10 citation statements)

References 7 publications

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“…Allele frequencies for autosomal STRs were retrieved from the GEP‐ISFG reference allele set used in collaborative exercises (http://www.gep-isfg.org/es/control-calidad/resultados-ejercicio-2015.html), Spanish allele frequencies deposited in STRBASE v2.0 (http://strbase.org), or Caucasian allele frequencies . Haplotype frequencies for linked X‐chromosome STRs, as well as allele frequencies for DXS7423, were retrieved from published data .…”

Section: Methodsmentioning

confidence: 99%

An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939)

et al. 2016

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Next-generation sequencing technologies have opened new opportunities in forensic genetics. Here, we assess the applicability and performance of the MiSeq FGx™ & ForenSeq™ DNA Signature Prep Kit (Illumina) for the identification of individuals from the mass graves of the Spanish Civil War (1936-1939). The main limitations for individual identification are the low number of possible first-degree living relatives and the high levels of DNA degradation reported in previous studies. Massively parallel sequencing technologies enabling the analysis of hundreds of regions and prioritizing short length amplicons constitute a promising tool for this kind of approaches. In this study, we first explore the power of this new technology to detect first- and second-degree kinship given different scenarios of DNA degradation. Second, we specifically assess its performance in a set of low DNA input samples previously analyzed with CE technologies. We conclude that this methodology will allow identification of up to second-degree relatives, even in situations with low sequencing performance and important levels of allele drop-out; it is thus a technology that resolves previous drawbacks and that will allow a successful approximation to the identification of remains.

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Section: Methodsmentioning

confidence: 99%

An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939)

et al. 2016

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“…We used 50 loci: one half of them are routinely used in commercial kits (CSF1PO, D1S1656, D2S441, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, FGA, PENTA D, PENTA E, SE33, TH01, TPOX and vWA); the other is not (D1GATA113, D1S1677, D2S1776, D3S3053, D3S4529, D4S2364, D4S2408, D5S2500, D6S474, D6S1017, D6S1043, D9S1122, D10S1435, D11S4463, D12ATA63, D14S1434, D17S974, D17S1301, D18S853, D20S482, F13A1, F13B, FES, LPL, PENTA B, PENTA C). The Italian allelic frequencies of these loci were retrieved from http://www.allstr.de/allstr/home.seam; for D6S1043, Penta B and Penta C we used the Caucasian frequencies reported by Phillips et al [2]. The input of the algorithm is a column vector of described alleles frequencies for a given locus.…”

Section: Methodsmentioning

confidence: 99%

The Sibship Index can’t discriminates Half-Sibs from Full-Sibs; a new algorithm, including maternal genotype, can do it

Cardillo¹

2018

Preprint

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Sibship DNA testing is conducted in order to determine if two subjects share one or both biological parents: Full-sibs have both parents in common, whereas Half-sibs have one parent in common, either the mother or the father. When an alleged father is not available for a paternity test, sibship testing is one way to determine family relationships. Results of a sibship test have also been accepted as proof in Social Security benefit and other inheritance claims. To analyze the possibility that the siblings share two common parents versus only one common parent, the ratio of the full sibship index versus half sibship index is computed: a sibship index less than 1, provides genetic evidence which does not favor a full-sibling relationship. In this paper we will demonstrate that the actually used Sibship Index is unable to distinguish Full-sibs from Half-Sibs and the only way to distinguish them is to use a new Index including the genotype of the Mother, that, usually, is the known common parent.HighlightsUsually used Sibship Index (SI) greatly overestimates likelihood ratios;Rising up the number of investigated STR, all Half-Sibs are declared Full-Sibs;The only way to avoid this overestimate is to use a Sibship Index Corrected by the common parent genotype, that usually is the mother (SICMG); all likelihood ratio formulas can be reconducted to five scenarios;For both SI and SICMG there is a better cut-off than 1 to discriminate Half-Sibs: this cut-off is computed for all commercial available forensic STR kit.

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“…The loci of Promega's CS7 supplementary STR kit were considered insufficiently polymorphic to merit detailed analysis and have already been studied for linkage status and population variation [14,20]…”

Section: Autosomal Strs Of Potential Interest But Not Incorporated Inmentioning

confidence: 99%

A genomic audit of newly-adopted autosomal STRs for forensic identification

Phillips

2017

Forensic Science International: Genetics

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In preparation for the growing use of massively parallel sequencing (MPS) technology to genotype forensic STRs, a comprehensive genomic audit of 73 STRs was made in 2016 [Parson et al., Forensic Sci. Int. Genet. 22, 54-63]. The loci examined included miniSTRs that were not in widespread use, but had been incorporated into MPS kits or were under consideration for this purpose. The current study expands the genomic analysis of autosomal STRs that are not commonly used, to include the full set of developed miniSTRs and an additional 24 STRs, most of which have been recently included in several supplementary forensic multiplex kits for capillary electrophoresis. The genomic audit of these 47 newly-adopted STRs examined the linkage status of new loci on the same chromosome as established forensic STRs; analyzed world-wide population variation of the newly-adopted STRs using published data; assessed their forensic informativeness; and compiled the sequence characteristics, repeat structures and flanking regions of each STR. A further 44 autosomal STRs developed for forensic analyses but not incorporated into commercial kits, are also briefly described.

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Global population variability in Promega PowerPlex CS7, D6S1043, and Penta B STRs

Cited by 11 publications

References 7 publications

An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939)

An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939)

The Sibship Index can’t discriminates Half-Sibs from Full-Sibs; a new algorithm, including maternal genotype, can do it

A genomic audit of newly-adopted autosomal STRs for forensic identification

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