Global population variability in Qiagen Investigator HDplex STRs

Fernandez-Formoso, L.; Gelabert-Besada, Miguel; García-Magariños, Manuel; Amigo, Jorge; Carracedo, Ángel; Lareu, M.V.

doi:10.1016/j.fsigen.2013.07.006

Cited by 20 publications

(13 citation statements)

References 14 publications

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“…Sixteen (88.89%) ambiguous alleles were determined using the GlobalFiler™ PCR Amplification kit, which includes 3 STR loci (D12S391, D18S51, and SE33) shared with the Investigator HDplex kit. Although the remaining two peaks (D3S1744 and D5S2500) could be separated by peak height ratio, we confirmed the alleles through additional single-plex PCR using the reported primers [5].…”

supporting

confidence: 58%

“…The Investigator Ⓡ HDplex kit (QIAGEN, Hilden, Germany) was developed to discriminate between related individuals for specific forensic applications and paternity testing, is complementary to the core STR markers [2,5]. Forensic genetic studies using the Investigator Ⓡ HDplex kit have been performed in different laboratories [2,[4][5][6][7][8].…”

mentioning

confidence: 99%

“…Forensic genetic studies using the Investigator Ⓡ HDplex kit have been performed in different laboratories [2,[4][5][6][7][8]. To date, the use of the Investigator Ⓡ HDplex kit to calculate the forensic genetic parameters of the Korean population has not been reported.…”

mentioning

confidence: 99%

“…DNA (0.2~1.0 ng) was amplified with the Investigator Ⓡ HDplex kit according to the manufacturer's protocols. To confirm alleles falling outside of the marker size range, additional PCR was performed using the GlobalFiler™ PCR Amplification kit (Thermo Fisher Scientific, Waltham, MA) as described by the manufacturer, or using single-plex PCR (D3S1744 and D5S2500) with primer sequences, as described by Phillips et al [5]. Forward primers were labeled with FAM, and known alleles of the CCR 9948 commercial control DNA were used as makers to confirm allele size.…”

mentioning

confidence: 99%

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Evaluation of forensic genetic parameters of 12 STR loci in the Korean population using the InvestigatorⓇ HDplex kit

Jung

Kim

et al. 2017

Int J Legal Med

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We genotyped and calculated the forensic parameters of 10 non-CODIS loci and 2 CODIS loci of 990 Korean individuals using the Investigator HDplex kit. No significant deviations from Hardy-Weinberg equilibrium (after Bonferroni correction for multiple testing) or genetic linkage disequilibrium were observed. The calculated matching probability and power of discrimination ranged from 0.0080 to 0.2014, and 0.7986 to 0.9920, respectively. We conclude that the markers of the kit are highly informative corroborative tools for forensic DNA analysis.

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supporting

confidence: 58%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of forensic genetic parameters of 12 STR loci in the Korean population using the InvestigatorⓇ HDplex kit

Jung

Kim

et al. 2017

Int J Legal Med

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“…Second, supplementary kits of STRs complimentary to the above core sets have been released. These consist of the 7‐plex Promega CS‐7 kit with five novel STRs—LPL, F13B, FESFPS, F13A01, Penta C, and the 12‐plex Qiagen Investigator HDplex kit —with nine novel STRs—D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D21S2055. The definition of “core” forensic STRs is largely shaped by any one country's choice of NDNAD (national DNA database) markers, for example, SE33 is a core marker in Germany and Austria, but has intermittent use elsewhere despite showing a number of desirable properties for forensic profiling .…”

Section: More Strs Per Test—extending the Core Forensic Multiplexesmentioning

confidence: 99%

“New turns from old STaRs”: Enhancing the capabilities of forensic short tandem repeat analysis

et al. 2014

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The field of research and development of forensic STR genotyping remains active, innovative, and focused on continuous improvements. A series of recent developments including the introduction of a sixth dye have brought expanded STR multiplex sizes while maintaining sensitivity to typical forensic DNA. New supplementary kits complimenting the core STRs have also helped improve analysis of challenging identification cases such as distant pairwise relationships in deficient pedigrees. This article gives an overview of several recent key developments in forensic STR analysis: availability of expanded core STR kits and supplementary STRs, short-amplicon mini-STRs offering practical options for highly degraded DNA, Y-STR enhancements made from the identification of rapidly mutating loci, and enhanced analysis of genetic ancestry by analyzing 32-STR profiles with a Bayesian forensic classifier originally developed for SNP population data. As well as providing scope for genotyping larger numbers of STRs optimized for forensic applications, the launch of compact next-generation sequencing systems provides considerable potential for genotyping the sizeable proportion of nucleotide variation existing in forensic STRs, which currently escapes detection with CE.

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Investigator HDplex markers: allele frequencies and mutational events in a North Italian population

et al. 2014

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Autosomal short tandem repeats (STRs) analysis represents the method of election in forensic genetics and up to now, 23 STRs are available for these purposes. However, in particular circumstances such as human identification or complex kinship cases, examination of additional STRs may be required in order to obtain reliable conclusions. For this purpose, a new multiplex STR system, namely Investigator® HDplex kit (QIAGEN) that coamplifies a set of 12 autosomal loci, 9 of which, represents novel supplementary STRs, was recently developed. A population sample of 359 unrelated healthy subjects residing in North Italy was typed to determine allele frequencies, forensic parameters and genetic distances among European populations. Furthermore, to evaluate the suitability of the HDplex kit as an auxiliary tool for paternity testing, mutation rates were estimated on 84 confirmed family trios. The 12 loci resulted highly informative with a combined power of discrimination of 0.999998 and no departures from Hardy-Weinberg equilibrium were observed with the sole exception of locus D4S2366. From the comparison of our population sample and European reference populations, a single significant difference was revealed with the Poland population at D4S2366 locus. With regard to the mutation rate study, on a total of 2,016 meioses considered, six single-step mutational events were observed and the average mutation rate calculated was of 2.94 × 10(-3) per locus per generation (95% confidence interval, 1.08 × 10(-3)-6.39 × 10(-3)).

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Global population variability in Qiagen Investigator HDplex STRs

Cited by 20 publications

References 14 publications

Evaluation of forensic genetic parameters of 12 STR loci in the Korean population using the InvestigatorⓇ HDplex kit

Evaluation of forensic genetic parameters of 12 STR loci in the Korean population using the InvestigatorⓇ HDplex kit

“New turns from old STaRs”: Enhancing the capabilities of forensic short tandem repeat analysis

Investigator HDplex markers: allele frequencies and mutational events in a North Italian population

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