Glomerular expression of connexin 40 and connexin 43 in rat experimental glomerulonephritis

Morioka, Tetsuo; Okada, Shinichi; Nameta, Masaaki; Kamal, Fadia; Yanakieva-Georgieva, Nadia; Yao, Jian; Sato, Ayako; Piao, Honglan; Oite, Takashi

doi:10.1007/s10157-012-0687-2

Cited by 18 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is accumulating evidence that GJs play crucial role in renal physiology, as alteration of GJ activity contributes to structural and functional damage, leading to several kidney diseases [12][13][14][15]73]. In this paper, the major relevant processes that are indispensable for the A.…”

Section: Discussionmentioning

confidence: 99%

“…Cx37 was found only in mesangial cells at the vascular pole of the glomerulus, while Cx43 was detected in mesangial cells of rat glomeruli [7,13,24].…”

Section: Mesangial Cellsmentioning

confidence: 99%

“…Cx43 is abundantly produced by human and rat podocytes [10,13], but is only present in small quantities in mouse podocytes (unpublished observations). In addition, a single study reported Cx45 staining in peripheral glomerular cells, presumably podocytes [26].…”

Section: Podocytesmentioning

confidence: 99%

“…Since then, several studies demonstrated the expression of some members of the Cx family in all renal cell types in humans and rodents [2][3][4][5][6][7][8]. Although impairment of GJs and hemichannels (HCs) has been reported to exert substantial impact in renal diseases [9][10][11][12][13][14][15], our knowledge regarding the involvement of GJs in renal physiology is still limited. In addition, even though recent studies have associated high or decreased pannexin (Panx) expression with a wide range of human diseases, their role in kidney is poorly known [16].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Functional roles of connexins and pannexins in the kidney

Abed

Kavvadas

Chadjichristos

2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Kidneys are highly complex organs, playing a crucial role in human physiopathology, as they are implicated in vital processes, such as fluid filtration and vasomotor tone regulation. There is growing evidence that gap junctions are major determinants of renal physiopathology. It has been demonstrated that their expression or channel activity may vary depending on physiological and pathological situations within distinct renal compartments. While some studies have focused on the role of connexins in renal physiology, our knowledge regarding the functional relevance of pannexins is still very limited. In this paper, we provide an overview of the involvement of connexins, pannexins and their channels in various physiological processes related to different renal compartments.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Cx37 was found only in mesangial cells at the vascular pole of the glomerulus, while Cx43 was detected in mesangial cells of rat glomeruli [7,13,24].…”

Section: Mesangial Cellsmentioning

confidence: 99%

Section: Podocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional roles of connexins and pannexins in the kidney

Abed

Kavvadas

Chadjichristos

2015

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…Cx40 expression in mesangial cells is related to mesangial cell regeneration, suggesting that connexin expression could be a therapeutic target for glomerular disease [43].…”

Section: The Role Of Connexins In the Diabetic Kidneymentioning

confidence: 99%

Mind the gap: connexins and cell–cell communication in the diabetic kidney

2014

View full text Add to dashboard Cite

Connexins, assembled as a hexameric connexon, form a transmembrane hemichannel that provides a conduit for paracrine signalling of small molecules and ions to regulate the activity and function of adjacent cells. When hemichannels align and associate with similar channels on opposing cells, they form a continuous aqueous pore or gap junction, allowing the direct transmission of metabolic and electrical signals between coupled cells. Regulation of gap junction synthesis and channel activity is critical for cell function, and a number of diseases can be attributed to changes in the expression/function of these important proteins. Diabetic nephropathy is associated with several complex metabolic and inflammatory responses characterised by defects at the molecular, cellular and tissue level. In both type 1 and type 2 diabetes, glycaemic injury of the kidney is the leading cause of end-stage renal failure, a consequence of multiple aetiologies, including increased deposition of extracellular matrix, glomerular hyperfiltration, albuminuria and tubulointerstitial fibrosis. In diabetic nephropathy, loss of connexin mediated cell-cell communication within the nephron may represent an early sign of disease; however, our current knowledge of the role of connexins in the diabetic kidney is sparse. This review highlights recent evidence demonstrating that maintained connexin-mediated cell-cell communication could benefit region-specific renal function in diabetic nephropathy and suggests that these proteins should be viewed as a tantalising novel target for therapeutic intervention.

show abstract

Connexin mRNA distribution in adult mouse kidneys

Geis

Boudriot

Wagner

2021

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

Kidneys are thought to express eight different connexin isoforms (i.e., Cx 26, 30, 32, 37, 40, 43, 45, and 46), which form either hemichannels or gap junctions serving to intercellular communication and functional synchronization. Proper function of connexins has already been shown to be crucial for regulation of renal hemodynamics and renin secretion, and there is also growing evidence for connexins to play a role in pathologic conditions such as renal fibrosis or diabetic nephropathy. Therefore, exact intrarenal localization of the different connexin isoforms gains particular interest. Until now intrarenal expression of connexins has mainly been examined by immunohistochemistry, which in part generated conflicting results depending on antibodies and fixation protocols used. In this work, we used fluorescent RNAscope as an alternative technical approach to localize renal connexin mRNAs in healthy mouse kidneys. Addition of RNAscope probes for cell type specific mRNAs was used to assign connexin mRNA signals to specific cell types. We hereby found Cx26 mRNA strongly expressed in proximal tubules, Cx30 mRNA was selectively detected in the urothelium, and Cx32 mRNA was found in proximal tubules and to a lesser extent also in collecting ducts. Cx37 mRNA was mainly associated with vascular endothelium, Cx40 mRNA was largely found in glomerular mesangial and less in vascular endothelial cells, Cx43 mRNA was sparsely expressed by interstitial cells of all kidney zones, and Cx45 mRNA was predominantly found in smooth muscle cell layers of both blood vessels and ureter as well as in mesangial and interstitial (fibroblastic) cells. Cx46 mRNA could not be detected. In summary our results essentially confirm previous data on connexin expression in the renal vasculature and in glomeruli. In addition, they demonstrate strong connexin gene expression in proximal tubules, and they suggest significant connexin expression in resident tubulointerstitial cells.

show abstract

Glomerular expression of connexin 40 and connexin 43 in rat experimental glomerulonephritis

Abstract: These results suggest that Cx40 expression in mesangial cells is related to mesangial cell regeneration. Thus, Cx expression regulation could be a therapeutic target for glomerular diseases.

Cited by 18 publications

References 39 publications

Functional roles of connexins and pannexins in the kidney

Functional roles of connexins and pannexins in the kidney

Mind the gap: connexins and cell–cell communication in the diabetic kidney

Connexin mRNA distribution in adult mouse kidneys

Contact Info

Product

Resources

About