GlpD involved in the virulence and persistence of a marine pathogen Vibrio splendidus

Li, Weisheng; Li, Ya; Shi, Weibo; Zhang, Weiwei

doi:10.1016/j.aquaculture.2024.740691

Cited by 1 publication

(1 citation statement)

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“…In the case of nutrient limiting Marine M9 media (MM9) containing glycerol as the sole carbon source, the hexR mutant growth defect was further exacerbated (Fig 5B). Glycerol is metabolized into dihydroxyacetone-phosphate (DHAP) which can enter either the glycolytic or gluconeogenic pathways [34]. While the wild type V. parahaemolyticus strain did have an extended lag phase compared to rich (LB) conditions, the sigmoidal growth trend remained the same with a maximum 𝑂𝐷 600𝑛𝑚 of 0.054.…”

Section: Vp1236 Is An Important Regulator For Growth On Diverse Nutri...mentioning

confidence: 99%

Functional genomics of chitin degradation byVibrio parahaemolyticusreveals finely integrated metabolic contributions to support environmental fitness

Getz,

Robinson,

Thomas

2024

Preprint

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Vibriospecies are marine prokaryotes that inhabit diverse ecological niches, colonizing abiotic and biotic surfaces. These bacteria are vital players in the global carbon cycle, assimilating billions of tonnes of chitin for carbon (and nitrogen) metabolites. Many bacterial proteins involved in the process-including chitinases, sugar transporters, and modifying enzymes-have been well studied. However, the genetic functional interplay and key drivers ofVibriocompetitive survival in the presence of chitin as the dominant carbon source is not understood. To address this question, we carried out transposon sequencing (Tn-seq) to determine the genetic fitness ofVibrio parahaemolyticusmutants grown on chitin as a sole carbon source. Along with validating knownVibriogenes associated with chitin metabolism, our data newly identified vital roles for an unclassified OprD-like import porin and a HexR family transcriptional regulator. Furthermore, we functionally implicated HexR in regulating multiple physiological processes involved inV. parahaemolyticusenvironmental survival including carbon assimilation and cell growth, biofilm formation, and cell motility. Notably, our data revealed a strict requirement for HexR in filamentous cell morphology, a critical trait forV. parahaemolyticusenvironmental fitness. Therefore, a vital import porin and genomic regulation mediated by HexR supports multiple physiological processes forVibriochitinolytic growth and environmental fitness.

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Section: Vp1236 Is An Important Regulator For Growth On Diverse Nutri...mentioning

confidence: 99%

Functional genomics of chitin degradation byVibrio parahaemolyticusreveals finely integrated metabolic contributions to support environmental fitness

Getz,

Robinson,

Thomas

2024

Preprint

View full text Add to dashboard Cite

show abstract

GlpD involved in the virulence and persistence of a marine pathogen Vibrio splendidus

Cited by 1 publication

References 57 publications

Functional genomics of chitin degradation byVibrio parahaemolyticusreveals finely integrated metabolic contributions to support environmental fitness

Functional genomics of chitin degradation byVibrio parahaemolyticusreveals finely integrated metabolic contributions to support environmental fitness

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