Glucocorticoids and Insulin Promote the Differentiation of Human Adipocyte Precursor Cells into Fat Cells

Hauner, Hans; Schmid, Peter; Pfeiffer, Ernst-Friedrich

doi:10.1210/jcem-64-4-832

Cited by 286 publications

(191 citation statements)

References 15 publications

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“…Cross-reactions with this receptor is indeed possible when insulin is added at high concentrations [45,140]. Because no or poor effects are observed with physiological concentrations, similar interpretation concerns human and rabbit preadipocytes [105,182]. However, it has been shown more recently that high concentrations of insulin are not obligatory for the differentiation of 3T3-L1 cells [223].…”

Section: Insulin Igf-1 and Ghmentioning

confidence: 98%

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The adipose conversion process: regulation by extracellular and intracellular factors

Boone

Mourot

Gregoire³

et al. 2000

Reprod. Nutr. Dev.

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-White adipose tissue regulates lipid metabolism and acts as a secretory organ. Because of its importance for human health and animal production, many studies have attempted to better understand its development at the cellular and molecular levels by culturing preadipose cells in vitro. This synthesis article describes our current knowledge, acquired by this approach, concerning the regulation of the different steps of the adipocyte differentiation program by extracellular (hormones, cytokines, growth factors, retinoids and fatty acids) and intracellular agents (second messengers and transcription factors). The discrepant effects that have been observed for some of these factors are also discussed. This information is very important in the perspective of a better control of fat deposits in human and breeding species.preadipocyte / differentiation / hormonal agent / second messenger / transcription factor Résumé -Le processus d'adipoconversion : sa régulation par des facteurs extracellulaires et intracellulaires. Le tissu adipeux blanc régule le métabolisme lipidique et agit comme un organe de sécrétion. Étant donné son importance pour la santé humaine et la production animale, de nombreuses études ont tenté de mieux comprendre son développement aux niveaux cellulaire et moléculaire en utilisant des cultures de préadipocytes. Cet article de synthèse décrit notre connaissance actuelle, issue de cette approche, concernant la régulation des différentes étapes du programme de la diffé-renciation adipocytaire par des facteurs extracellulaires (hormones, cytokines, facteurs de croissance, rétinoïdes et acides gras) et intracellulaires (seconds messagers et facteurs de transcription). Les effets divergents observés pour certains de ces facteurs sont également discutés. Ces informations sont très importantes dans la perspective d'un meilleur contrôle des dépôts adipeux chez l'humain et les espèces d'élevage.préadipocyte / différenciation / hormone / second messager / facteur de transcription Reprod. Nutr. Dev. 40 (2000) 325-358 325

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Section: Insulin Igf-1 and Ghmentioning

confidence: 98%

“…These hormones are adipogenic in both preadipose cell lines and primary preadipocytes, whatever the conditions of culture [42,80,90,103,105,176,182,189,227].…”

Section: The Glucocorticoidsmentioning

confidence: 99%

The adipose conversion process: regulation by extracellular and intracellular factors

Boone

Mourot

Gregoire³

et al. 2000

Reprod. Nutr. Dev.

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“…However, interest in adipose tissue has increased after mesenchymal stem cells, also known as "human adipose tissue derived stem cells (hADMSCs)", were isolated from this tissue (Deslex et al, 1987;Hauner et al, 1987;Pettersson et al, 1984). hADMSCs are multipotent stem cells that are recovered from the stromal vascular fraction (SVF) of adipose tissue (Gimble et al, 2007;Nakagami et al, 2006;Ning et al, 2006;Psaltis et al, 2008;Schäffler et al, 2007;Wilson et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Many types of primary cells are isolated from adipose tissue but the stromal vascular fractions predominantly contain circulating blood cells, endothelial cells, pericytes, adipocyte progenitor cells, and smooth muscle progenitor cells (Gimble et al, 2007). This heterogeneous cell population can be cultured under different conditions, but with standard culture conditions specifically tailored for hADMSCs the cells generally form "spindle" or "fibroblast-like" morphologies as they are passaged (Deslex et al, 1987;Hauner et al, 1987;Pettersson et al, 1984;Zuk et al, 2001). This finding indicates that some types of cells such as endothelial and circulating blood cells disappear under these culture conditions.…”

mentioning

confidence: 99%

Phenotypic Characterization and In Vivo Localization of Human Adipose-Derived Mesenchymal Stem Cells

Ryu

Cho

Lee

et al. 2013

Molecules and Cells

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Human adipose-derived mesenchymal stem cells (hADMSCs) are a potential cell source for autologous cell therapy due to their regenerative ability. However, detailed cytological or phenotypic characteristics of these cells are still unclear. Therefore, we determined and compared cell size, morphology, ultrastructure, and immunohistochemical (IHC) expression profiles of isolated hADMSCs and cells located in human adipose tissues. We also characterized the localization of these cells in vivo. Light microscopy examination at low power revealed that hADMSCs acquired a spindle-shaped morphology after four passages. Additionally, high power views showed that these cells had various sizes, nuclear contours, and cytoplasmic textures. To further evaluate cell morphology, transmission electron microscopy was performed. hADMSCs typically had ultrastructural characteristics similar to those of primitive mesenchymal cells including a relatively high nuclear/cytosol ratio, prominent nucleoli, immature cytoplasmic organelles, and numerous filipodia. Some cells contained various numbers of lamellar bodies and lipid droplets. IHC staining demonstrated that PDGFR and CD10 were constitutively expressed in most hADMSCs regardless of passage number but expression levels of α-SMA, CD68, Oct4 and c-kit varied. IHC staining of adipose tissue showed that cells with immunophenotypic characteristics identical to those of hADMSCs were located mainly in the perivascular adventitia not in smooth muscle area. In summary, hADMSCs were found to represent a heterogeneous cell population with primitive mesenchymal cells that were mainly found in the perivascular adventitia. Furthermore, the cell surface markers would be CD10/PDGFR. To obtain defined cell populations for therapeutic purposes, further studies will be required to establish more specific isolation methods.

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“…Glucocorticoids have a trophic effect on adipose tissue (8,9) and have a dose dependent ability to stimulate the differentiation of adipocyte precursors into adipocytes in the presence of insulin (50,103). H ypercortisolemia elevates adipocyte lipoprotein lipase activity two to threefold (96).…”

Section: Introductionmentioning

confidence: 99%

The Hypothalamic‐Pituitary‐Adrenal Axis in Obesity

1995

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Glucocorticoids and Insulin Promote the Differentiation of Human Adipocyte Precursor Cells into Fat Cells

Cited by 286 publications

References 15 publications

The adipose conversion process: regulation by extracellular and intracellular factors

The adipose conversion process: regulation by extracellular and intracellular factors

Phenotypic Characterization and In Vivo Localization of Human Adipose-Derived Mesenchymal Stem Cells

The Hypothalamic‐Pituitary‐Adrenal Axis in Obesity

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