Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs

Zhao, Xin; Al-Qwbani, Mohammed; Luo, Yue; Chen, Changjun; A, Ge; Wei, Yang; Li, Donghai; Wang, Qiuru; Meng, Tian; Kang, Pengde

doi:10.1111/jcmm.16103

Cited by 16 publications

(17 citation statements)

References 55 publications

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“…In this study, the rats with GC administration had almost invisible structural trabeculae and decreased BV and BMD in the subchondral bone. Further histological study showed that the necrotic area was occupied by non-structural chondrocytes, which is consistent with the findings of several studies, [22,23] but differs from others that showed large numbers of empty lacunae filling the bone trabeculae, as well as disordered, sparser, or destroyed bone trabeculae [24,25] . Since Morini et al [26] observed that subchondral trabeculae originated from the ossification of the diaphysis and do not start before the third month, we speculate that the differences in the histological studies were likely due to different treatments and different sampling times.…”

Section: Discussionsupporting

confidence: 89%

“…Further histological study showed that the necrotic area was occupied by non-structural chondrocytes, which is consistent with the findings of several studies, [ 22 , 23 ] but differs from others that showed large numbers of empty lacunae filling the bone trabeculae, as well as disordered, sparser, or destroyed bone trabeculae. [ 24 , 25 ] Since Morini et al [ 26 ] observed that subchondral trabeculae originated from the ossification of the diaphysis and do not start before the third month, we speculate that the differences in the histological studies were likely due to different treatments and different sampling times. In addition, the rats that received GC treatment accompanied by lithium presented with fewer pathological changes and relatively normal trabecular structures, together with better trabecular parameters, indicating the protective effect of lithium against bone loss in femoral heads induced by GC.…”

Section: Discussionmentioning

confidence: 96%

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Lithium chloride prevents glucocorticoid-induced osteonecrosis of femoral heads and strengthens mesenchymal stem cell activity in rats

Zhang

Zhu

Zhang

et al. 2021

Chinese Medical Journal

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Background: Accumulating evidence suggests that lithium influences mesenchymal stem cell (MSC) proliferation and osteogenic differentiation. As decreased bone formation in femoral heads is induced by glucocorticoids (GCs), we hypothesized that lithium has a protective effect on GC-induced osteonecrosis of femoral heads (ONFH). Methods: A rat ONFH model was induced by methylprednisolone (MP) and the effect of lithium chloride on the models was evaluated. Micro-computed tomography (CT)-based angiography and bone scanning were performed to analyze the vessels and bone structure in the femoral heads. Hematoxylin and eosin and immunohistochemical staining were performed to evaluate the trabecular structure and osteocalcin (OCN) expression, respectively. Bone marrow-derived MSCs were isolated from the models, and their proliferative and osteogenic ability was evaluated. Western blotting and quantitative real-time polymerase chain reaction were performed to detect osteogenic-related proteins including Runx2, alkaline phosphatase, and Collagen I. Results: Micro-CT analysis showed a high degree of osteonecrotic changes in the rats that received only MP injection. Treatment with lithium reduced this significantly in rats that received lithium (MP + Li group); while 18/20 of the femoral heads in the MP showed severe osteonecrosis, only 5/20 in the MP + Li showed mild osteonecrotic changes. The MP + Li group also displayed a higher vessel volume than the MP group (0.2193 mm 3 vs . 0.0811 mm 3 , P < 0.05), shown by micro-CT-based angiography. Furthermore, histological analysis showed better trabecular structures and more OCN expression in the femoral heads of the MP + Li group compared with the MP group. The ex vivo investigation indicated higher proliferative and osteogenic ability and upregulated osteogenic-related proteins in MSCs extracted from rats in the MP + Li group than that in the MP group. Conclusions: We concluded that lithium chloride has a significant protective effect on GC-induced ONFH in rats and that lithium also enhances MSC proliferation and osteogenic differentiation in rats after GC administration.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 96%

Lithium chloride prevents glucocorticoid-induced osteonecrosis of femoral heads and strengthens mesenchymal stem cell activity in rats

Zhang

Zhu

Zhang

et al. 2021

Chinese Medical Journal

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“…Glucocorticoid-induced ONFH is a common metabolic disorder of bone, which is often caused by the serious side effects of long-term use or overdose of glucocorticoid drugs. There have been proposed several potential hypotheses to account for the glucocorticoid-induced ONFH initiation and progression, including the differentiation imbalance of BMSCs, apoptosis of osteocytes and osteoblasts, abnormal intraosseous pressure, increased oxidative stress, etc., 23 , 24 , 25 , 26 although until now, the specific underlying mechanism involved in ONFH pathogenesis and development remains unclear, limiting the diagnosis and treatment strategies for ONFH. BMSCs are a subset of adult stem cells derived from mesodermal cell lineages, and they have strong potential in multi-lineage differentiation and self-renewal capacity.…”

Section: Discussionmentioning

confidence: 99%

CircHGF suppressed cell proliferation and osteogenic differentiation of BMSCs in ONFH via inhibiting miR-25-3p binding to SMAD7

Feng

Xiang

Jia

et al. 2022

Molecular Therapy - Nucleic Acids

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“…The modified lipopolysaccharide and hormone modeling method was used to establish a model of hormone-induced osteonecrosis in rabbits ( Zhao et al, 2020 ). Firstly, the rabbits were injected with 10 μg/kg of lipopolysaccharide (LPS; Sigma) intravenously.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo Repair Effects of the NCF-Col-NHA Aerogel Scaffold Loaded With SOST Monoclonal Antibody and SDF-1 in Steroid-Induced Osteonecrosis

Luo

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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In the current study, we synthesized nanocellulose (NCF)-collagen (Col)-nano hydroxyapatite (NHA) organic-inorganic hybrid aerogels loaded with stromal cell derived factor-1 (SDF-1) and sclerostin monoclonal antibody (SOST McAb) and investigated their ability to repair steroid-induced osteonecrosis. Rabbit bone marrow mesenchymal stem cells (BMSCs) and human vascular endothelial cells (HUVECs) were used for the in vitro study. A rabbit steroid-induced osteonecrosis model was used for the in vivo study. The best elastic modulus reached 12.95 ± 4.77 MPa with a mean compressive property of 0.4067 ± 0.084 MPa for the scaffold containing 100% mass fraction. The average pore diameter of the aerogel was 75 ± 18 µm with a porosity of more than 90% (96.4 ± 1.6%). The aerogel-loaded SDF-1 and SOST were released at 40–50% from the material within the initial 3 h and maintained a stable release for more than 21 days. The in vitro study showed osteogenesis and vascularization capabilities of the scaffold. The in vivo study showed that rabbits received implantation of the scaffold with SOST McAb and SDF-1 showed the best osteogenesis of the osteonecrosis zone in the femoral head. Imaging examination revealed that most of the necrotic area of the femoral head was repaired. These results suggest that this hybrid aerogel scaffold could be used for future steroid-induced osteonecrosis repair.

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Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 16 publications

References 55 publications

Lithium chloride prevents glucocorticoid-induced osteonecrosis of femoral heads and strengthens mesenchymal stem cell activity in rats

Lithium chloride prevents glucocorticoid-induced osteonecrosis of femoral heads and strengthens mesenchymal stem cell activity in rats

CircHGF suppressed cell proliferation and osteogenic differentiation of BMSCs in ONFH via inhibiting miR-25-3p binding to SMAD7

In vitro and in vivo Repair Effects of the NCF-Col-NHA Aerogel Scaffold Loaded With SOST Monoclonal Antibody and SDF-1 in Steroid-Induced Osteonecrosis

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