Glucocorticoids elevate the level of enkephalin‐like peptides in neuroblastoma × glioma hybrid cells

Gläser, Thomas; Hübner, Karin; Hamprecht, Bernd

doi:10.1016/0014-5793(81)80888-5

Cited by 13 publications

(6 citation statements)

References 25 publications

(31 reference statements)

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“…Dexamethasone alone has been shown to increase concentrations of the two opiate peptides; methionine-and leucineenkephalin [20]. In contrast to this study the response was rapid, occurring within the first 24 h of exposure to dexamethasone.…”

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confidence: 95%

Neuropeptide Y in neuroblastoma × glioma hybrid cells

et al. 1983

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High concentrationsof a newly-identified biologically potent peptide, neuropeptide Y, have been demonstrated in 3 related mouse neuroblastoma-derived donal cell lines, N18TG2 0.35 pmol/mg protein, NGlOS-15 0.44 pmol/mg protein and NCB-20 0.39 pmol/mg protein. The NG108-15 cell line was chosen for further evaluation. Dexametbasone (10&M) and nerve growth factor (10 ng/ml) resulted in a 2-fold increase in cellular neuropeptide Y concentrations. The response to dexamethasone was demonstrated to be dose-dependent. Exposure to both agents in combination resulted in a more than additive effect, indicating synergism.

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confidence: 95%

Neuropeptide Y in neuroblastoma × glioma hybrid cells

et al. 1983

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“…Neuroblastoma X glioma hybrid cell line, NG108-15, can be regarded as one of the best models for neuronal cells. NGlO8-15 cells synthesize and release acetylcholine [I], synthesize an enkephalin-like peptide [2], form functional synapses with skeletal muscle cells in culture [ 1,3] synthesize the promoting factor of acetylcholine receptor aggregation in muscle cells [4], and have many kinds of receptors [5-81. Therefore, it is worthwhile to study membrane properties of NG108-15 cells in order to investigate the mechanisms of the differentiation of neuronal cells.…”

Section: Introductionmentioning

confidence: 99%

The change in membrane fluidity of neuroblastoma × glioma hybrid cells upon cell differentiation

Kawasaki

Amano

1982

FEBS Letters

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“…The glucocorticoid effect was maximal after 3 days of exposure to 10 pM dexamethasone (Table 1). In cells cultured for 24 ht in the presence of 1 ,uM dexamethasone, the increase in proEnk mRNA was statistically significant, whereas TyrOHase mRNA increased significantly only after 5 days of exposure to the highest concentration of dexamethasone tested (1 ,uM) ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…Three peaks were observed. The first two were identified as [Met]Enk-Arg-Phe (fractions [12][13][14] and peptide B (fractions [16][17][18][19][20][21][22][23][24] (Fig. 2); the ratio of [Met]Enk-Arg-Phe to peptide 13 content remained constant for both the cells and the medium.…”

Section: Resultsmentioning

confidence: 99%

Permissive effect of dexamethasone on the increase of proenkephalin mRNA induced by depolarization of chromaffin cells.

Naranjo¹,

Mocchetti²,

Schwartz³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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In cultured bovine chromaffin cells, changes in the dynamic state of enkephalin stores elicited experimentally were studied by measuring cellular proenkephaliu mRNA, as well as enkephalin precursors and authentic enkephalin content of cells and culture media. In parallel, tyrosine hydroxylase mRNA and catecholamine cell content were also determined. Low concentrations (0.5-100 pM) of dexamethasone increased the cell contents of proenkephalin mRNA and enkephalin-containing peptides. High concentrations of the hormone (1 MuM) were required to increase the cell contents of tyrosine hydroxylase mRNA and catecholamines. Depolarization of the cells with 10 ,uM veratridine resulted in a depletion of enkephalin and catecholamine stores after 24 hr. The enkephalin, but not the catecholamine, content was restored by 48 hr. An increase in proenkephalin mRNA content might account for the recovery; this increase was curtailed by tetrodotoxin and enhanced by 10 pM dexamethasone. Tyrosine hydroxylase mRNA content was not significantly modified by depolarization, even in the presence of 1 MuM dexamethasone. Aldosterone, progesterone, testosterone, or estradiol (1 MuM) failed to change proenkephalin mRNA. Hence, dexamethasone appears to exert a specific permissive action on the stimulation of the proenkephalin gene elicited by depolarization. Though the catecholamines and enkephalins are localized in the same chromaffin granules and are coreleased by depolarization, the genes coding for the processes that are rate limiting in the production of these neuromodulators can be differentially regulated.Catecholamines and enkephalin-related peptides are colocalized in adrenal chromaffin cells (1). These peptides reside in the same storage vesicles with epinephrine and can be released by the same stimuli (2-5), suggesting that the stores of both neuromodulators might be regulated by common mechanisms. Glucocorticoids function as a physiological stimulus of adrenal medulla and control positively the transcription of different genes by the interaction of the steroid-receptor complex with specific sites of the genome upstream from the transcription starting site or within the transcriptional unit (6-9). Since, in primary cultures of chromaffin cells, depolarizing stimuli increase the accumulation of proenkephalin (proEnk) mRNA (10) but fail to increase significantly the content of tyrosine hydroxylase (TyrOHase) (11), we wondered whether there was a differential role of the glucocorticoids in the expression of these two genes.When chromaffin cells are cultured in hypoglucocorticoid media, low concentrations (picomolar or less) of dexamethasone significantly increase the synthesis of enkephalinrelated peptides and the content of proEnk mRNA. In contrast, TyrOHase mRNA and catecholamine content are barely increased by micromolar concentrations of the hormone. Furthermore, veratridine (10 ,uM) increases proEnk mRNA content and this increase is potentiated by 10 pM dexamethasone. Depolarization does not modify the amount of TyrOHase ...

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Glucocorticoids elevate the level of enkephalin‐like peptides in neuroblastoma × glioma hybrid cells

Cited by 13 publications

References 25 publications

Neuropeptide Y in neuroblastoma × glioma hybrid cells

Neuropeptide Y in neuroblastoma × glioma hybrid cells

The change in membrane fluidity of neuroblastoma × glioma hybrid cells upon cell differentiation

Permissive effect of dexamethasone on the increase of proenkephalin mRNA induced by depolarization of chromaffin cells.

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