The change in membrane fluidity of neuroblastoma × glioma hybrid cells upon cell differentiation

Kawasaki, Yuki; Amano, Takehiko

doi:10.1016/0014-5793(82)80533-4

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…RESULTS Table 1 shows the lateral diffusion coefficients of F18 embedded into cell membranes of normal and SV40-transformed fibroblasts. These values were close to those of membrane lipids [10,11] and were almost identical to those obtained in other cells [4][5][6][7]. Therefore, the motion of F18 may represent that of membrane lipids and the membrane fluidity, as described by the lateral mobility of F18, was similar in various cell types.…”

Section: Measurementssupporting

confidence: 87%

“…The diameter of the bleached area was 3.8/zm using a 100 × objective lens and a pinhole. In order to reduce the effect of rotational motion, a polarizer and a 1/4 wavelength phase plate were inserted along the path of exciting light as described in [4]. Only the uniformly labelled regions of cell surfaces were selectively measured in order to reduce the effect of aggregation and internalization of the probes.…”

Section: Measurementsmentioning

confidence: 99%

“…The lateral mobility of fluorescent molecules was measured by FPR. Instrumental description and analysis are given in [4]. The diameter of the bleached area was 3.8/zm using a 100 × objective lens and a pinhole.…”

Section: Measurementsmentioning

confidence: 99%

“…In order to obtain more clear information on the dynamic properties of cell membranes, combination of the data of rotational and lateral mobility is required. The lateral mobility of FI8 and S-F-concanavalin A is very sensitive to the structural changes of cell membranes from neuronal cells [4][5][6] and muscle cells [7]. Here we report that the lateral mobility of fluorescent molecules on cell membranes measured by the FPR technique is different depending on probes used: : the lateral mobility of fluorescent fatty acid analog was lower in transformed fibroblasts, whereas, the lateral mobility of S-F-concanavalin A remained unchanged.…”

Section: Introductionmentioning

confidence: 99%

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Reduced lateral mobility of a fluorescent lipid analog in cell membranes of rat fibroblasts transformed by simian virus 40

et al. 1989

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In order to study the difference between normal and transformed cells, lateral motion of fluorescent molecules embedded into cell membranes of rat clonal fibroblasts and its SV40-transformed derivative cells was measured by the FPR technique. The lateral diffusion coefficient of a fluorescent fatty acid analog, F 18, was smaller in transformed cells than normal cells. This indicates that the lipid phase of membranes from transformed cells is less fluid than that from normal cells. On the other hand, the lateral diffusion coefficient of S-F-concanavalin A was identical in both cells. These results suggest that the mobility of different molecules on the membranes is controlled by different mechanisms.

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Section: Measurementssupporting

confidence: 87%

Section: Measurementsmentioning

confidence: 99%

Section: Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Reduced lateral mobility of a fluorescent lipid analog in cell membranes of rat fibroblasts transformed by simian virus 40

et al. 1989

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“…The instrumental description and analysis are shown in [13]. The diameter of the bleached area was 2.8 pm using a 100 x objective and a pinhole.…”

Section: Measurementsmentioning

confidence: 99%

Lateral motion of fluorescent molecules embedded into cell membranes of clonal myogenic cells, L6, changes upon cell maturation

1988

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The lateral motion of fluorescent molecules embedded into cell membranes of myogenic cell line, L6, was measured. The motion of S-F-ConA became faster at cell fusion stage, and became slower after fusion. On the other hand, the motion of lipid analog, F18, was not changed at cell fusion stage. However, after fusion when myotubes were formed, the motion of F18 became slower. At cell fusion stage, there was a large variation in the motion of S-F-ConA.This means that at this stage the properties of myoblasts change drastically and rapidly.

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Effect of fatty acids on the membrane fluidity of cultured chick dorsal root ganglion measured by fluorescence photobleaching recovery

1983

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The effect of fatty acids on the membrane fluidity in tissue cultured chick embryo dorsal root ganglion was studied by fluorescence recovery method. Lateral motion of the lipid was measured by observing the fluorescent probe, 5-(octadecylthiocarbamoylamino) fluorescence, F18. The effective lateral diffusion coefficient of the membrane was around 0.30 X 10(-8) cm2/sec in control cells, 0.42 X 10(-8) cm2/sec in 2-decenoic acid treated cells, and 0.35 X 10(-8) cm2/sec in valeric acid treated cells. From these results it is concluded that effective mobilities of the membrane complex increased about 40% by the external application of 2-decenoic acid, while valeric acid increased it only 12%. From the physiological results that 2-decenoic acid inhibits the Na-channel, it is suggested that this increase in the membrane fluidity might affect the Na-channel.

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The change in membrane fluidity of neuroblastoma × glioma hybrid cells upon cell differentiation

Cited by 6 publications

References 18 publications

Reduced lateral mobility of a fluorescent lipid analog in cell membranes of rat fibroblasts transformed by simian virus 40

Reduced lateral mobility of a fluorescent lipid analog in cell membranes of rat fibroblasts transformed by simian virus 40

Lateral motion of fluorescent molecules embedded into cell membranes of clonal myogenic cells, L6, changes upon cell maturation

Effect of fatty acids on the membrane fluidity of cultured chick dorsal root ganglion measured by fluorescence photobleaching recovery

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