Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head

Yu, Qing; Guo, Wanshou; Cheng, Liming; Lü, Yongtao; Shen, Jianying; Li, Ping

doi:10.4103/0366-6999.160564

Cited by 27 publications

(29 citation statements)

References 22 publications

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“…Another interesting finding was that the numbers of down-regulated In-cRNAs are unexceptionally more than that of up-regulated IncRNAs in each chromosome, and the result of mRNA is quite the opposite. Although this result is consistent with previous other investigations (Bu et al, 2012;Zhu et al, 2015), Yu et al reported that there were more down-regulated IncRNAs than up-regulated In-cRNAs in the glucocorticoids-treated bone microvascular endothelial cells and conversely, differentially upregulated mRNAs were more common than significantly down-regulated mRNAs (Yu et al, 2015). The precise regulatory mechanism remains unclear and further studies are needed to illuminate the exact mechanisms.…”

Section: Discussionsupporting

confidence: 92%

“…In the current study, we classified the dysregulated IncRNAs and discovered that 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were sRNA host IncRNAs and 0.05% were enhancer ( Figure 2D). Among the successfully annotated IncRNAs, more intergenic transcripts than antisense or intronic transcripts were differentially expressed, which were consistent with previous other investigations Yu et al, 2015).…”

Section: General Characteristics Of Dysregulated Incrnassupporting

confidence: 91%

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The long non-coding RNA expression profile of Coxsackievirus A16 infected RD cells identified by RNA-seq

Shi

Xiong

et al. 2016

Virol. Sin.

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Coxsackievirus A16 (CVA16) is one of major pathogens of hand, foot and mouth disease (HFMD) in children. Long non-coding RNAs (IncRNAs) have been implicated in various biological processes, but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of IncRNAs of normal and CVA16 infected rhabdomyosarcoma (RD) cells using RNA-Seq to investigate the functional relevance of IncRNAs. We showed that a total of 760 IncRNAs were upregulated and 1210 IncRNAs were downregulated. Out of these dysregulated IncRNAs, 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were sRNA host IncRNAs and 0.05% were enhancer. Six dysregulated IncRNAs were validated by quantitative PCR assays and the secondary structures of these IncRNAs were projected. Moreover, we conducted a bioinformatics analysis of an IncRNAs (ENST00000602478) to elucidate the diversity of modification and functions of IncRNAs. In summary, the current study compared the dysregulated IncRNAs profile upon CVA16 challenge and illustrated the intricate relationship between coding and IncRNAs transcripts. These results may not only provide a complete picture of transcription in CVA16 infected cells but also provide novel molecular targets for treatments of HFMD.

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Section: Discussionsupporting

confidence: 92%

Section: General Characteristics Of Dysregulated Incrnassupporting

confidence: 91%

The long non-coding RNA expression profile of Coxsackievirus A16 infected RD cells identified by RNA-seq

Shi

Xiong

et al. 2016

Virol. Sin.

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“…The dysfunction of BMECs has been reported to cause femoral head microcirculation obstacle, which is key to the development of glucocorticoid-induced ONFH [10]. Yu et al [19] reported that glucocorticoids profoundly affect the transcriptome of BMECs during the early stages of glucocorticoid-induced ONFH. According to Yue et al [18], glucocorticoids can induce BMECs to express various cytokines, which contributes to the development of glucocorticoid-induced ONFH.…”

Section: Discussionmentioning

confidence: 99%

Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head

Liu

Zuo

et al. 2020

BMC Musculoskelet Disord

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Background: Glucocorticoid-induced osteonecrosis of the femoral head (ONFH) is closely associated with the dysfunction of the bone microvascular endothelial cells (BMECs). The present study investigated the angiogenic and apoptotic activity of the BMECs in glucocorticoid-induced ONFH. Methods: This study enrolled a total of 12 patients, six of whom were assigned to the ONFH group whereas the other six served as the control group. The ONFH group was composed of patients with glucocorticoid-induced ONFH while the control group had femoral neck fractures. BMECs were isolated from the subchondral region of the femoral head. Cell proliferation, cell viability, tube formation assay, Transwell assay, TUNEL assay, and Western blot analysis were performed.Results: BMECs of the two groups were successfully isolated and identified. No significant differences were noticed in BMECs proliferation between the two groups. However, compared to the control, cell viability, tube formation, and migration of BMECs were significantly decreased and the number of TUNEL positive cells was markedly increased in the ONFH group. In the ONFH group, it was also noted that the amount of Bax and cleaved-caspase3 was elevated while that of Bcl-2 was reduced. Conclusion:The findings of our study revealed that BMECs obtained from the glucocorticoid-induced ONFH patients had decreased angiogenic and increased apoptotic activities, which could explain the pathogenesis and progression of glucocorticoid-induced ONFH.

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“…There have been only a few studies concerning the correlation between the expression of lncRNAs and AVNFH. Yu et al 28…”

Section: Discussionmentioning

confidence: 99%

Microarray analysis of long‐noncoding RNAs and mRNA expression profiles in human steroid‐induced avascular necrosis of the femoral head

Luo

Lan

et al. 2019

J of Cellular Biochemistry

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This study performed the first microarray analysis of long‐noncoding RNA (lncRNA) and mRNA expression profiles in human steroid‐induced avascular necrosis of the femoral head (SAVNFH). Expression levels of lncRNAs and mRNAs in three human SAVNFH samples and three human femoral head fracture samples (controls) were detected using third‐generation lncRNA microarrays (KangChen Biotech, Shanghai, China). The fold change, false discovery rate, and P value were utilized to filter genes with significant differential expression in the SAVNFH samples compared with the control samples. In total, there were 1179 upregulated and 3214 downregulated lncRNAs (P2. zerofold, P < 0.05). Meanwhile, 1092 upregulated and 565 downregulated mRNAs were found in the SAVNFH samples compared with the control samples. Then, quantitative real‐time polymerase chain reaction was used to confirm the previous microarray results using 8 and 20 selected dysregulated lncRNAs and mRNAs, respectively, and the results generally confirmed the microarray findings. Finally, we used Gene Ontology (GO) and pathway analysis to investigate the functions of the altered mRNAs and their associated GO terms and biological pathways. The Immune system process term (GO:0002376) was the most significantly upregulated GO term, and the Regulation of blood coagulation term (GO:0030193) was the most significantly downregulated GO term in the biological process category for the SAVNFH samples. “Hematopoietic cell lineage ‐ Homo sapiens (human) (Pathway ID: hsa04640)” and “Complement and coagulation cascades ‐ Homo sapiens (human) (Pathway ID: hsa04610)” were the most significantly up‐ and downregulated pathways in the SAVNFH samples compared with the controls. In conclusion, the differential expression of lncRNAs and mRNAs may be correlated with the pathogenesis of SAVNFH, and these significantly dysregulated lncRNAs and mRNAs may function through networks or participate in several specific biological processes. Further research is needed to understand their exact functions and mechanisms in SAVNFH.

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Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head

Cited by 27 publications

References 22 publications

The long non-coding RNA expression profile of Coxsackievirus A16 infected RD cells identified by RNA-seq

The long non-coding RNA expression profile of Coxsackievirus A16 infected RD cells identified by RNA-seq

Decreased angiogenic and increased apoptotic activities of bone microvascular endothelial cells in patients with glucocorticoid-induced osteonecrosis of the femoral head

Microarray analysis of long‐noncoding RNAs and mRNA expression profiles in human steroid‐induced avascular necrosis of the femoral head

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