Microarray analysis of long‐noncoding RNAs and mRNA expression profiles in human steroid‐induced avascular necrosis of the femoral head

Abstract

This study performed the first microarray analysis of long‐noncoding RNA (lncRNA) and mRNA expression profiles in human steroid‐induced avascular necrosis of the femoral head (SAVNFH). Expression levels of lncRNAs and mRNAs in three human SAVNFH samples and three human femoral head fracture samples (controls) were detected using third‐generation lncRNA microarrays (KangChen Biotech, Shanghai, China). The fold change, false discovery rate, and P value were utilized to filter genes with significant differential expression in the SAVNFH samples compared with the control samples. In total, there were 1179 upregulated and 3214 downregulated lncRNAs (P2. zerofold, P < 0.05). Meanwhile, 1092 upregulated and 565 downregulated mRNAs were found in the SAVNFH samples compared with the control samples. Then, quantitative real‐time polymerase chain reaction was used to confirm the previous microarray results using 8 and 20 selected dysregulated lncRNAs and mRNAs, respectively, and the results generally confirmed the microarray findings. Finally, we used Gene Ontology (GO) and pathway analysis to investigate the functions of the altered mRNAs and their associated GO terms and biological pathways. The Immune system process term (GO:0002376) was the most significantly upregulated GO term, and the Regulation of blood coagulation term (GO:0030193) was the most significantly downregulated GO term in the biological process category for the SAVNFH samples. “Hematopoietic cell lineage ‐ Homo sapiens (human) (Pathway ID: hsa04640)” and “Complement and coagulation cascades ‐ Homo sapiens (human) (Pathway ID: hsa04610)” were the most significantly up‐ and downregulated pathways in the SAVNFH samples compared with the controls. In conclusion, the differential expression of lncRNAs and mRNAs may be correlated with the pathogenesis of SAVNFH, and these significantly dysregulated lncRNAs and mRNAs may function through networks or participate in several specific biological processes. Further research is needed to understand their exact functions and mechanisms in SAVNFH.

Proteomics analysis of hip articular cartilage identifies differentially expressed proteins associated with osteonecrosis of the femoral head

“…Complement and coagulation cascades was identified to be upregulated in ONFH cartilage, which reveals that they are involved in ONFH cartilage degeneration. This change was also observed in transcription analysis of bone tissue and serum proteome in patients with ONFH, respective 42,43 . Further investigation is expected about how these pathways are involved in the development of ONFH.…”

Research Progress of Tonifying Kidney and Promoting Blood Circulation in the Treatment of Steroid-induced Necrosis of Femoral Head

“…Osteocyte apoptosis is one of the most recognized causes of this disease at present. Studies have shown that the use of glucocorticoids can increase the death of osteoblasts and osteocytes and destroy the mechanical sensation of the bone cell network [26] . Bone trabeculae are sparse, bone mass is reduced, and osteonecrosis occurs.…”

Therapeutic perspectives of exosomes in glucocorticoid-induced osteoarthrosis

“…Recent research results indicate that lncRNA also plays an important regulatory role in the pathogenesis and repair of ONFH. LncRNA was found to be differentially expressed in ONFH tissues, bone marrow mesenchymal stem cells and bone microvascular endothelial cells which isolated from ONFH patients ( 9 , 107 , 108 ). Functional research has further clarified its important role in the survival of osteoblasts closely related to ONFH and the osteogenic differentiation of bone marrow mesenchymal stem cells.…”