Glucose-6-Phosphatase and α-Glucosidase Inhibitors from Smilax moranensis Roots Identified by Affinity-Directed Fractionation

Identification of Hypoglycemic Glycolipids from Ipomoea murucoides by Affinity-Directed Fractionation, In Vitro, In Silico and Dynamic Light Scattering Analysis

“…The application of affinity screening, involving the incubation of the methanolic extract with G6Pase and the subsequent employment of the Size-Exclusion Chromatography/Electrospray Ionization Mass Spectrometry (SEC/ESI-MS) protocol [ 11 , 15 ], facilitated the detection of signals at m/z 1029.5568 (5%), 1127.6646 (11%), and 1175.6113 (3%) on a high-resolution (HR) spectrometer ( Figure 2 ). These signals correspond to five of the nineteen murucoidins previously isolated in I. murucoides through their cationized molecules [M + Na] + [ 2 , 4 ]: murucoidins XV and XVI with a molecular weight of 1006 amu (1029 [M + Na] + ), murucoidin XIV with a molecular weight of 1104 amu (1127 [M + Na] + ), and murucoidin III and stoloniferin I with a molecular weight of 1152 amu (1175 [M + Na] + ).…”

“…This analysis predicted that all docked compounds formed hydrogen bonds with amino acid residues that are part of the catalytic site, primarily with HIS279 in MAL12 ( Figure 5 a, Table 1 ) and preserved catalytic residues around TYR1251 in MGAM ( Figure 5 b, Table 1 ), which is involved in the catalytic substrate specificity of this protein [ 11 , 15 ]. These results indicate that the structure of the pentasaccharide stoloniferin I is the best conformation of the glycolipids selected for the inhibition of the two enzymes under study ( Table 1 ), resulting in a lower concentration value for the theoretical Ki for MAL12 (244 mM) and for MGAM (4.28 mM).…”

“…The affinity-directed fractionation methods used in the present investigation were previously described in [ 11 , 15 ]. Gel permeation chromatography was performed with a spin column packed with polyacrylamide (BioRad Laboratories, Hercules, CA, USA).…”

The complete chloroplast genome and phylogenetic analysis of Smilax moranensis (Liliales: Smilacaceae)

“… 2017 ; Rosas-Ramirez et al. 2020 ). Despite the fact that S. moranensis has significant medicinal value, its research in genetics and evolution is still extremely rare.…”

“…Smilax moranensis M.Martens & Galeotti 1842 is a woody vine with stems covered in spikes and is widely distributed in warm and temperate climates between 600 and 2000 m above sea level (Rosas-Ramirez et al. 2020 ). Several reports about the root of S. moranensis detail its pharmacological activities for treating rheumatic joint pain (Xu et al.…”