Glucose induced break down of enhanced biological phosphate removal

Čech, J. S.; Hartman, Petr

doi:10.1080/09593339009384907

Cited by 114 publications

(56 citation statements)

References 5 publications

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“…The occurrence of coccoid bacteria with a distinctive tetrad morphology first surfaced in activated sludge systems in the early 1990s (Cech & Hartman, 1990). These were Gramnegative cocci and were commonly seen in reactors fed with an acetate/glucose mixture.…”

mentioning

confidence: 99%

Quadrisphaera granulorum gen. nov., sp. nov., a Gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules

Maszenan

Tay

Schümann

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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A Gram-positive bacterium, designated strain AG019T, was isolated by micromanipulation from aerobic granules obtained from a laboratory-scale sequencing batch reactor. This isolate grew axenically as cocci clustered predominantly in tetrads, and was morphologically similar to the dominant organisms observed in the biomass. The morphology also resembled that of the tetrad-forming organisms commonly seen in activated sludge samples. Strain AG019T was found to be an oxidase-negative, catalase-positive, non-motile aerobe that does not reduce nitrate and grows at temperatures between 15 and 40 °C, with an optimum at 37 °C. The pH range for growth was 5·0–9·0, with an optimum at pH 7·5. Strain AG019T contained a peptidoglycan with directly cross-linked meso-diaminopimelic acid (type A1γ) and lacked mycolic acids. The G+C content of the DNA was 75 mol%. Menaquinone MK-8(H2) was the major isoprenoid quinone. The bacterium stained positively for intracellular polyphosphate granules but not for poly-β-hydroxyalkanoates. It produced capsular material and showed autoaggregation ability. Phenotypic and 16S rRNA gene analyses showed that the bacterium differed sufficiently from its closest phylogenetic relatives, namely members of the suborder Frankineae, which includes the genera Geodermatophilus, Blastococcus, Frankia, Sporichthya, Acidothermus and Microsphaera, that it is proposed that it be placed in a novel genus, Quadrisphaera, as Quadrisphaera granulorum gen. nov., sp. nov. The type strain is AG019T (=ATCC BAA-1104T=DSM 44889T).

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mentioning

confidence: 99%

Quadrisphaera granulorum gen. nov., sp. nov., a Gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules

Maszenan

Tay

Schümann

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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“…Furthermore, Dv1-GAOs and Dv2-GAOs appeared within Alphaproteobacteria and constituted 3.5% and 2.7% of all bacteria, respectively. It should be noted that among Alphaproteobacteria mostly TFOs were present, which are often associated with EBPR deterioration [8,11,12,27,48]. Interestingly, significantly less bacteria (34% of the EUBmix hybridized cells) was not covered by the oligonucleotide probes used in the current study, when compared to the 61% of bacteria that did not hybridize with the specific probes at the beginning of the experiment.…”

Section: Resultsmentioning

confidence: 64%

“…Glucose was used in the current study despite reports of the failure of lab-scale reactors fed with this substrate [8,34]. Relatively high concentrations of glucose may induce the proliferation of TFOs that are associated with the deterioration of EBPR systems, because this substrate can replace internally stored glycogen and serve as the energy source (instead of poly-P) and reducing power for PHA accumulation [4].…”

Section: Wastewater Compositionmentioning

confidence: 99%

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From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

et al. 2013

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A laboratory scale sequencing batch reactor (SBR), fed with synthetic wastewater containing a mixture of organic compounds, was operated for nearly six months. Despite maintaining the same operational conditions, a deterioration of enhanced biological phosphorus removal (EBPR) occurred after 40 days of SBR operation. The Prel/Cupt ratio decreased from 0.28 to 0.06 P-mol C-mol−1, and C requirements increased from 11 to 32 mg C h−1 g−1 of mixed liquor suspended solids. A FISH analysis showed that the percentage of Accumulibacter in an overall community of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) dropped from 93% to 13%. An increase in abundance of Gammaproteobacteria (from 2.6% to 22%) and Alphaproteobacteria (from 1.8% to 10%) was observed. The number of Competibacter increased from 0.5% to nearly 9%. Clusters 1 and 2 of Defluviicoccus-related GAOs, not detected before deterioration, constituted 35% and 27% of Alphaproteobacteria, respectively. We concluded that lab-scale experiments should not be extended implicitly to full-scale EBPR systems because some bacterial groups are detected mainlyin lab-scale reactors. Well-defined, lab-scale operational conditions reduce the number of ecological niches available to bacteria.

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“…Thus it reduced the utilization efficiency of denitrifying phosphorus removal bacteria PK1 to glucose, which would further influence the PO4 3--P and COD removal efficiency. On the other hand, Carucci [12] and Cech [13] have reported that glucose would replace the intracellular glycogen to provide energy and reduce the biological growth; therefore, it promoted the growth of GAOs selectively and reduced the competitiveness of PAOs, thus affecting the absorption of phosphorus. Fig.…”

Section: Advances In Engineering Research Volume 120mentioning

confidence: 99%

Influence of nutrient factors on phosphorus accumulating organisms (PAOs) Bacillus sp. PK1

Zhang¹,

Yao²,

Li³

et al. 2018

Proceedings of the 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017)

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A kind of phosphorus accumulating organism (PAO), Bacillus sp. PK1, which showed high phosphorus removal efficiencies, was isolated from special medium in the SBR reactor. To further explore the effect of nutrient factors on its potential phosphorus removal efficiency, initial phosphorus content and various carbon sources were selected in this research. Results of a series tests showed that the initial phosphorus removal efficiency declined constantly to below 60% with the initial phosphorus content increased. As for the effect of carbon sources, the phosphorus removal efficiencies were respectively as high as 98.58%, 95.14% and 98.45% with sodium acetate, sodium citrate and mixed carbon source. More importantly, research also showed an infrequent phenomenon that the PK1 gave priority to utilize sodium citrate rather than short chain fatty acid sodium acetate, and hardly to use glucose.

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Glucose induced break down of enhanced biological phosphate removal

Cited by 114 publications

References 5 publications

Quadrisphaera granulorum gen. nov., sp. nov., a Gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules

Quadrisphaera granulorum gen. nov., sp. nov., a Gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules

From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

Influence of nutrient factors on phosphorus accumulating organisms (PAOs) Bacillus sp. PK1

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