Glucose oxidase: an ideal enzyme

Wilson, Robert; Turner, Anthony P. F.

doi:10.1016/0956-5663(92)87013-f

Cited by 1,261 publications

(488 citation statements)

References 118 publications

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“…The majority of commercially available glucose biosensors utilise GOx due to its relatively 30 low price, high selectivity and stability [62,63]. GOx is a flavoprotein that catalyses the oxidation of β-D-glucose to D-glucono-δ-lactone, which is then hydrolyzed to gluconic acid [64].…”

Section: Enzyme Based Glucose Biosensorsmentioning

confidence: 99%

An overview of dealloyed nanoporous gold in bioelectrochemistry

Xiao

Magner

2016

Bioelectrochemistry

113

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Section: Enzyme Based Glucose Biosensorsmentioning

confidence: 99%

An overview of dealloyed nanoporous gold in bioelectrochemistry

Xiao

Magner

2016

Bioelectrochemistry

113

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“…Ag/AgCl), causes the following reaction: H 2 O 2 → O 2 + 2H + + 2e − generating a measurable current that is proportional to the rate of the H 2 O 2 . The high specificity and stability of GOx makes this enzyme suitable for biosensor construction [5] when immobilized on the surface of platinum electrodes with poly-ophenylenediamine [6].…”

Section: Introductionmentioning

confidence: 99%

Design and construction of a low cost single-supply embedded telemetry system for amperometric biosensor applications

Serra

Rocchitta

Bazzu

et al. 2007

Sensors and Actuators B: Chemical

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A new embedded telemetry system for amperometric biosensor application is presented. The device consists of a single-supply miniature potentiostat-I/V converter, a microcontroller unit (MCU), a signal transmitter, and a stabilized power supply. The sensor current is converted to a digital value using a peripheral interface controller (PIC) MCU with an integrated analog-to-digital converter (ADC). The PIC firmware is developed in assembly and transferred to the MCU through an in-circuit-serial-programmer (ICSP). The digital data are sent to a personal computer using a miniaturized 433.92 MHz amplitude modulation (AM) transmitter with a linear range up to 30 m. The radio receiver is connected to a PC via a Universal Serial Bus (USB). Custom developed software, written in C and Basic, allows the PC to record, plot and handle the received data. The design, construction and operation of the hardware and software are described. The system performance was evaluated in vitro using a dummy cell and a platinum (Pt) amperometric glucose biosensor. This device serves as a basic model to realize an in vivo, low-cost, miniaturized telemetry system built with standard hardware components readily available.

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“…The most thoroughly investigated is the glucose biosensor incorporating the oxidoreductase enzyme, glucose oxidase (GOx). GOx, isolated from Aspergillus niger, has a high substrate specificity, a high turnover rate and excellent stability, that make it ideally suited for use in a biosensor (Wilson and Turner, 1992). This enzyme catalyses the oxidation of D-glucose (1)…”

Section: Introductionmentioning

confidence: 99%

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

Lowry

Miele

O’Neill

et al. 1998

Journal of Neuroscience Methods

100

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Amperometric glucose biosensors based on the immobilization of glucose oxidase (GOx) on Pt electrodes with electropolymerized o-phenylenediamine (PPD) were implanted in the right striatum of freely-moving rats. Carbon paste electrodes for the simultaneous monitoring of ascorbic acid (AA) and/or tissue O 2 were implanted in the left striatum. A detailed in vivo characterization of the Pt/PPD/GOx signal was carried out using various pharmacological manipulations. Confirmation that the biosensor responded to changing glucose levels in brain extracellular fluid (ECF) was obtained by intraperitoneal (i.p.) injection of insulin that caused a decrease in the Pt/PPD/GOx current, and local administion of glucose (1 mM) via an adjacent microdialysis probe that resulted in an increase in the biosensor current. An insulin induced increase in tissue O 2 in the brain was also observed. Interference studies involved administering AA and subanaesthetic doses of ketamine i.p. Both resulted in increased extracellular AA levels with ketamine also causing an increase in O 2 . No significant change in the Pt/PPD/GOx current was observed in either case indicating that changes in O 2 and AA, the principal endogenous interferents, have minimal effect on the response of these first generation biosensors. Stability tests over a successive 5-day period revealed no significant change in sensitivity. These in vivo results suggest reliable glucose monitoring in brain ECF.

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Glucose oxidase: an ideal enzyme

Cited by 1,261 publications

References 118 publications

An overview of dealloyed nanoporous gold in bioelectrochemistry

An overview of dealloyed nanoporous gold in bioelectrochemistry

Design and construction of a low cost single-supply embedded telemetry system for amperometric biosensor applications

An amperometric glucose-oxidase/poly(o-phenylenediamine) biosensor for monitoring brain extracellular glucose: in vivo characterisation in the striatum of freely-moving rats

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