Glucose oxidase bound to self-assembled monolayers of bis(4-pyridyl) disulfide at a gold electrode: Amperometric determination of glucose

Murthy, A. S. N.; Sharma, Jyotsna

doi:10.1016/s0003-2670(98)00083-x

Cited by 56 publications

(38 citation statements)

References 17 publications

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“…The I max and K app M values were determined by analyzing the slope and intercept for the plot of the reciprocals of the current versus glucose concentration and found to be 6.78 mA (216.0 mA cm À2 ) and 3.76 mM, respectively. In addition, the K app M value in the present work is smaller than those of the immobilized GOD in sol-gel/ PVA-g-P(4-VP) (20 mM) [4], Pt/PB/GOD-Pan (10.3 mM) [40], sol-gel/chitosan composite (21 mM) [41], PB/sol-gel/ GOD (6.7 mM) [42] and polypyrrole (25.3 mM) [43], and that of 20 mM for GOD bound to self-assemble monolayer electrode [44]. It is clear that the immobilized GOD using our method possesses high catalytic activity with respect to glucose and rapid mass transport for analytes and products in the meantime.…”

Section: Biosensors Amperometric Characterizationmentioning

confidence: 99%

Preparation of GOD/Sol‐Gel Silica Film on Prussian Blue Modified Electrode for Glucose Biosensor Application

2008

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A novel amperometric glucose biosensor was fabricated by in situ incorporating glucose oxidase (GOD) within the sol-gel silica film on a Prussian blue (PB) modified electrode. The method is simple and controllable, which combined the merits of in situ immobilizing biomolecules in sol-gel silica film by electrochemical method and the synergic catalysis effects of PB and GOD molecules. Scanning electron microscopy (SEM) showed that the GOD/sol-gel silica film was homogeneous with a large number of three-dimensional nanopores, which not only enhanced mass transport, but also maintained the active configuration of the enzyme molecule and prevented the leakage of enzyme, therefore improved the stability and sensitivity of the biosensor. The fabricated biosensor showed fast response time (10 s), high sensitivity (26.6 mA cm À2 M À1), long-term stability, good suppression of interference, and linear range of 0.01 mM -5.8 mM with a low detection limit of 0.94 mM for the detection of glucose. In addition, the biosensor was successfully applied to determine glucose in human serum samples.

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Section: Biosensors Amperometric Characterizationmentioning

confidence: 99%

Preparation of GOD/Sol‐Gel Silica Film on Prussian Blue Modified Electrode for Glucose Biosensor Application

2008

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“…The last possibility (third generation biosensors) is the direct electron transfer between the enzyme×s redox center and the electrode surface [11,12] Gold electrodes have been increasingly used in designing electrochemical biosensors because they allow durable immobilization of enzymes to the electrode surface while controlling the molecular architecture of the recognition layer, most often via binding to self-assembled monolayers (SAMs) made of sulfur-containing compounds [12 ± 15]. Glutaraldehyde coupling was also used to strengthen enzyme immobilization into the SAMs [16]. Thin-film flexible gold electrodes obtained by Au deposition on polymer substrates that were subsequently coated with an enzyme layer have been also used for this purpose [17 ± 19].…”

Section: Introductionmentioning

confidence: 99%

Flow Injection Amperometric Detection at Enzyme‐Modified Gold Nanoelectrodes

2004

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Gold nanotubular electrode ensembles were prepared by using electroless deposition of the metal within the pores of polycarbonate particle track-etched membranes. Glucose oxidase (Gox), used as a model enzyme, has been immobilized onto preformed self-assembled monolayers (mercaptoethylamine or mercaptopropionic acid) on electroless gold via cross-linking with glutaraldehyde or covalent attachment by carbodiimide coupling. Flowinjection analysis systems in flow-through or wall-jet configurations using these Gox-modified nanoelectrodes are described. The influence of different experimental parameters (i.e., applied potential, flow rate, interferents...) on the analytical response of the sensor to glucose has been evaluated. Under optimized conditions, very reproducible results (standard deviations < 4%, n 38) were obtained, linear calibration was achieved in the 2 Â 10 À4 M to 3 Â 10 À2 M concentration range and the detection limit was 2 Â 10 À4 M. Moreover, no significant interferences from species like ascorbic and uric acids were observed at a potential of 0.9 V.

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“…Apart from this, the application of functionalized SAMs has also been reported. [17][18][19] Recently, synthetic metalloporphyrins have attracted attention in relation to the chemical and biological recognition. 20,21 Electrodes prepared by incorporating metalloporphyrins are good candidates for various applications, because they have excellent thermal, chemical, electrochemical, and photochemical stability.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensing of Acyclovir at a Gold Electrode Modified with 2-Mercaptobenzothiazole–[5,10,15,20-tetrakis-(3-methoxy-4-hydroxyphenyl)porphyrinato]copper(II)

Joseph

Kumar

2011

ANAL. SCI.

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Glucose oxidase bound to self-assembled monolayers of bis(4-pyridyl) disulfide at a gold electrode: Amperometric determination of glucose

Cited by 56 publications

References 17 publications

Preparation of GOD/Sol‐Gel Silica Film on Prussian Blue Modified Electrode for Glucose Biosensor Application

Preparation of GOD/Sol‐Gel Silica Film on Prussian Blue Modified Electrode for Glucose Biosensor Application

Flow Injection Amperometric Detection at Enzyme‐Modified Gold Nanoelectrodes

Electrochemical Sensing of Acyclovir at a Gold Electrode Modified with 2-Mercaptobenzothiazole–[5,10,15,20-tetrakis-(3-methoxy-4-hydroxyphenyl)porphyrinato]copper(II)

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