Glucose Oxidase Immobilization on Guar Gum–Gelatin Dual-Templated Silica Hybrid Xerogel

Singh, Vandana; Singh, Devendra Kumar

doi:10.1021/ie402341c

Cited by 19 publications

(8 citation statements)

References 35 publications

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“…As shown in Table 1, the K m value of GOD−SiO 2 (7.73 mM) was smaller than that of free GOD (21.75 mM), which could be attributed to the favorable change in the GOD conformation or the decreased enzyme inhibition upon immobilization. 35 That is to say, the immobilization process can keep GOD in a more active conformation and then enhanced the binding affinity between GOD and glucose or made the formation of the enzyme− substrate complex easier. 35 However, the K m of CAT−SiO 2 (358.88 mM) was increased compared to that of free CAT (239.09 mM), which can be explained by the mass transfer limitation and/or reduction in enzyme−substrate affinity.…”

Section: Resultsmentioning

confidence: 99%

Pickering Emulsion Stabilized by Immobilized Bienzyme Nanoparticles: A Novel and Robust System for Enzymatic Purification of Isomaltooligosaccharide

Gao

Jiang

et al. 2014

Ind. Eng. Chem. Res.

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A bienzyme system aiming at removal of glucose from isomaltooligosaccharide (IMO) was developed in this study. In this bienzymatic system, the first enzyme (glucose oxidase, GOD) catalyzed glucose to generate H 2 O 2 and gluconic acid, while the second one (catalase, CAT) broke down the undesired H 2 O 2 to water and oxygen, which can reduce the peroxideinduced degradation of GOD. For improving the catalytic activity, realizing the recyclability, and simplifying the separation operations of the enzymes, a Pickering emulsion stabilized by GOD-and CAT-containing silica particles was constructed. The optimal reaction temperature and pH were identified as 45°C and 6.0, respectively. Under such conditions, the removal efficiency of glucose could be maintained at ca. 73.57%, even after five cycles in repeated batch operations. It is believed that the proposed process of glucose removal with biocatalytic Pickering emulsion is promising for the purification of IMO on an industrial scale.

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Section: Resultsmentioning

confidence: 99%

Pickering Emulsion Stabilized by Immobilized Bienzyme Nanoparticles: A Novel and Robust System for Enzymatic Purification of Isomaltooligosaccharide

Gao

Jiang

et al. 2014

Ind. Eng. Chem. Res.

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“…Alternatively, multiple enzymes on a single support may be used to perform a sequence of reactions as discussed below. We may anticipate further such examples in the future where several enzymes are loaded onto a single support [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Discussion: Novel Support Technologiesmentioning

confidence: 99%

Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox

2021

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Enzymes have been exploited by humans for thousands of years in brewing and baking, but it is only recently that biocatalysis has become a mainstream technology for synthesis. Today, enzymes are used extensively in the manufacturing of pharmaceuticals, food, fine chemicals, flavors, fragrances and other products. Enzyme immobilization technology has also developed in parallel as a means of increasing enzyme performance and reducing process costs. The aim of this review is to present and discuss some of the more recent promising technical developments in enzyme immobilization, including the supports used, methods of fabrication, and their application in synthesis. The review highlights new support technologies such as the use of well-established polysaccharides in novel ways, the use of magnetic particles, DNA, renewable materials and hybrid organic–inorganic supports. The review also addresses how immobilization is being integrated into developing biocatalytic technology, for example in flow biocatalysis, the use of 3D printing and multi-enzymatic cascade reactions.

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“…DHB was used as the redox mediator to transfer the electrons between the enzymatic glucose oxidation and the electrode [39]. Also, gelatin which was used to reduce GO and stabilized AuNPs can also help with the enzyme immobilization [40]. The modified electrode, denoted as rGO/AuNPs-GOx/DHB, was assembled and investigated for glucose biosensor.…”

Section: Optimization Of Nanocompositementioning

confidence: 99%

One-pot scalable synthesis of rGO/AuNPs nanocomposite and its application in enzymatic glucose biosensor

Sadak

2021

Nanocomposites

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A nanocomposite, rGO/AuNPs, was synthesized simultaneously using one-pot approach with gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) within gelatin which used as a reducing and stabilizing agent. Then, the fabricated nanocomposites were characterized by UV-Vis, transmission electron microscope (TEM) and field emission scanning electron microscope (FE-SEM). The optimal nanocomposite was determined using electrochemical approaches. After facile and eco-friendly synthesis of rGO/AuNPs nanocomposites, it was used to fabricate the bioanode of enzymatic glucose biosensors. After drop-casting the nanocomposites on a screen-printed electrode (SPE), the glucose oxidase (GOx) was immobilized on the pre-treated SPE through a protein cross-linking approach using glutaraldehyde (GA) as a crosslinking reagent and 2,5-dihydroxybenzaldehyde (DHB) as a mediator to improve the electrochemical performance. Then, electrochemical performance of enzyme immobilized nanocomposites was studied using the potentiostat. The results demonstrate that the enzymatic biosensor made of rGO/AuNPs nanocomposites showed enhanced the sensitivity of selectivity for detection of glucose.

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Glucose Oxidase Immobilization on Guar Gum–Gelatin Dual-Templated Silica Hybrid Xerogel

Cited by 19 publications

References 35 publications

Pickering Emulsion Stabilized by Immobilized Bienzyme Nanoparticles: A Novel and Robust System for Enzymatic Purification of Isomaltooligosaccharide

Pickering Emulsion Stabilized by Immobilized Bienzyme Nanoparticles: A Novel and Robust System for Enzymatic Purification of Isomaltooligosaccharide

Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox

One-pot scalable synthesis of rGO/AuNPs nanocomposite and its application in enzymatic glucose biosensor

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