Glucose Oxidase Immobilized on a Functional Polymer Modified Glassy Carbon Electrode and Its Molecule Recognition of Glucose

Abstract

In the present study, a glucose oxidase (GluOx) direct electron transfer was realized on an aminated polyethylene glycol (mPEG), carboxylic acid functionalized multi-walled carbon nanotubes (fMWCNTs), and ionic liquid (IL) composite functional polymer modified glassy carbon electrode (GCE). The amino groups in PEG, carboxyl groups in multi-walled carbon nanotubes, and IL may have a better synergistic effect, thus more effectively adjust the hydrophobicity, stability, conductivity, and biocompatibility of the composite functional polymer film. The composite polymer membranes were characterized by cyclic voltammetry (CV), ultraviolet-visible (UV-Vis) spectrophotometer, fluorescence spectroscopy, electrochemical impedance spectroscopy (EIS), and transmission electron microscopy (TEM), respectively. In 50 mM, pH 7.0 phosphate buffer solution, the formal potential and heterogeneous electron transfer constant (ks) of GluOx on the composite functional polymer modified GCE were −0.27 V and 6.5 s−1, respectively. The modified electrode could recognize and detect glucose linearly in the range of 20 to 950 μM with a detection limit of 0.2 μM. The apparent Michaelis-Menten constant (Kmapp) of the modified electrode was 143 μM. The IL/mPEG-fMWCNTs functional polymer could preserve the conformational structure and catalytic activity of GluOx and lead to high sensitivity, stability, and selectivity of the biosensors for glucose recognition and detection.

Glucose Biosensor Based on Glucose Oxidase Immobilized on BSA Cross-Linked Nanocomposite Modified Glassy Carbon Electrode

“…The effects of MWCNTs, HFs, and BSA on the catalytic activity of GOD were studied using an ultraviolet and visible spectrophotometer (UV-vis spectrophotometer) (Evolution 220, Thermo, Shanghai, China) [ 34 ].…”

MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination

“…TEM (JEM-2100, JEOL, Tokyo, Japan) images of MWCNTs, MWCNTs-CTAB, HFs, and HFs-Lac were taken at 200 KV [ 48 ]. The spectroscopic study of laccase catalyzing rutin was carried out on an ultraviolet and visible spectrophotometer (UV-vis spectrophotometer) (Evolution 220, Thermo, Shanghai, China).…”

Sensors to the Diagnostic Assessment of Anticancer and Antimicrobial Therapies Effectiveness by Drugs a with Pyrazine Scaffold

“…One of the most frequently used electrochemical methods is voltammetry with modified GCEs (unmodified electrodes are not preferred as they are characterized by a smaller range of linearity, lower limits of detection, and quantitation and a large overpotential). Numerous studies have shown that modification of electrodes with poly-L-amino, acid coatings, and/or coatings containing carbon nanoparticles improve their performance [23][24][25][26].…”

“…L-amino acid duplicated biosensors have also found application in the detection of drugs with a pyrazine motif. Research has been performed using differential impulse voltammetry on a sensor containing poly-L-methionine and electrochemically reduced graphene oxide/GCE to detect PZA in urine, plasma, and pharmaceuticals [24]. Similar stud- L-amino acid duplicated biosensors have also found application in the detection of drugs with a pyrazine motif.…”

“…Similar stud- L-amino acid duplicated biosensors have also found application in the detection of drugs with a pyrazine motif. Research has been performed using differential impulse voltammetry on a sensor containing poly-L-methionine and electrochemically reduced graphene oxide/GCE to detect PZA in urine, plasma, and pharmaceuticals [24]. Similar studies were carried out to estimate the content of PZA in serum using graphene oxide-or poly-L-arginine-modified GCE [30] and to determine PZA in pharmaceuticals, urine, and serum using tyrosine-modified GCE [31].…”