Glucose Response of Near-Infrared Alginate-Based Microsphere Sensors Under Dynamic Reversible Conditions

Chaudhary, Akshay; Härmä, Harri; Hänninen, Pekka; McShane, Michael J.; Srivastava, Rohit

doi:10.1089/dia.2011.0057

Cited by 13 publications

(11 citation statements)

References 33 publications

(55 reference statements)

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“…The current sensitivity exceeds that found in some prior results (0.04% per mg/dL) [58], but is lower than that found in others (0.18% per mg/dL) [53], for similar measurement ranges.…”

Section: Resultscontrasting

confidence: 83%

“…The analytical sensitivity—a characteristic parameter indicating how much change in the biosensor detected signal per unit change in GC [ 35 ]—was estimated to be 0.12% per mg/dL over the linear range of 0–400 mg/dL glucose. The current sensitivity exceeds that found in some prior results (0.04% per mg/dL) [ 58 ], but is lower than that found in others (0.18% per mg/dL) [ 53 ], for similar measurement ranges.…”

Section: Resultscontrasting

confidence: 83%

“…The linear range covers the clinically-relevant concentrations (preprandial: 70–130 mg/dL for glycemic control and >130 mg/dL for hyperglycemia; postprandial: <180 mg/dL for glycemic control and >180 mg/dL for hyperglycemia) [3]. A leveling off, or saturation, of the signal was observed as GC exceeded 400 mg/dL, an effect that has been seen in prior studies and is likely due to a high level of occupied protein binding sites [39,52,53,58]. Thus 400 mg/dL represents the limit of the useful range of the biosensor, and accuracy may degrade slightly for GCs at the very upper end of this range.…”

Section: Resultsmentioning

confidence: 93%

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In Vitro Evaluation of Fluorescence Glucose Biosensor Response

Aloraefy

Pfefer

Ramella‐Roman

et al. 2014

Sensors

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Rapid, accurate, and minimally-invasive glucose biosensors based on Förster Resonance Energy Transfer (FRET) for glucose measurement have the potential to enhance diabetes control. However, a standard set of in vitro approaches for evaluating optical glucose biosensor response under controlled conditions would facilitate technological innovation and clinical translation. Towards this end, we have identified key characteristics and response test methods, fabricated FRET-based glucose biosensors, and characterized biosensor performance using these test methods. The biosensors were based on competitive binding between dextran and glucose to concanavalin A and incorporated long-wavelength fluorescence dye pairs. Testing characteristics included spectral response, linearity, sensitivity, limit of detection, kinetic response, reversibility, stability, precision, and accuracy. The biosensor demonstrated a fluorescence change of 45% in the presence of 400 mg/dL glucose, a mean absolute relative difference of less than 11%, a limit of detection of 25 mg/dL, a response time of 15 min, and a decay in fluorescence intensity of 72% over 30 days. The battery of tests presented here for objective, quantitative in vitro evaluation of FRET glucose biosensors performance have the potential to form the basis of future consensus standards. By implementing these test methods for a long-visible-wavelength biosensor, we were able to demonstrate strengths and weaknesses with a new level of thoroughness and rigor.

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“…The current sensitivity exceeds that found in some prior results (0.04% per mg/dL) [58], but is lower than that found in others (0.18% per mg/dL) [53], for similar measurement ranges.…”

Section: Resultscontrasting

confidence: 83%

Section: Resultscontrasting

confidence: 83%

Section: Resultsmentioning

confidence: 93%

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In Vitro Evaluation of Fluorescence Glucose Biosensor Response

Aloraefy

Pfefer

Ramella‐Roman

et al. 2014

Sensors

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“…Left: glucose sensing mechanism for the long-wavelength probe reported by Chaudhary et al [102]; right: NIR fluorescent saccharide probes based on boronic acids and possible sensing mechanism.…”

Section: Figurementioning

confidence: 99%

Long-wavelength analyte-sensitive luminescent probes and optical (bio)sensors

Staudinger

Borisov

2015

Methods Appl. Fluoresc.

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Long-wavelength luminescent probes and sensors become increasingly popular. They offer the advantage of lower levels of autofluorescence in most biological probes. Due to high penetration depth and low scattering of red and NIR light such probes potentially enable measurements in tissues and some of them have already reached a high level of reliability required for such applications. This review focuses on the recent progress in development and application of long-wavelength analyte-sensitive probes which can operate both reversibly and irreversibly. Photophysical properties, sensing mechanisms, advantages and limitations of individual probes are discussed.

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“…For example, glucose microsensors and nanosensors provide the benefits of rapid response times and ease of implantation due to their large surface-area-to-volume ratio and small size. 6 Chaudhary and coauthors 7 are developing a “smart tattoo” composed of dissolved-core alginate microspheres to be implanted into the skin and monitor glucose levels in interstitial fluid. These sensors use fluorescence resonance energy transfer and a competitive binding mechanism with a noncatalytic mutant of glucose oxidase 8 to repeatedly monitor reversible changes in glucose at physiological levels.…”

Section: Introductionmentioning

confidence: 99%