Glucose Sensing Using Capacitive Biosensor Based on Polyvinylidene Fluoride Thin Film

Abstract

A polyvinylidene fluoride (PVDF) film-based capacitive biosensor was developed for glucose sensing. This device consists of a PVDF film sandwiched between two electrodes. A capacitive biosensor measures the dielectric properties of the dielectric layers at the interface between the electrolyte and the electrode. A glucose oxidase (GOx) enzyme was immobilized onto the electrode to oxidize glucose. In practice, the biochemical reaction of glucose with the GOx enzyme generates free electron carriers. Consequently, the potential difference between the electrodes is increased, resulting in a measurable voltage output of the biosensor. The device was tested for various glucose concentrations in the range of 0.013 to 5.85 M, and various GOx enzyme concentrations between 4882.8 and 2.5 million units/L. We found that the sensor output increased with increasing glucose concentration up to 5.85 M. These results indicate that the PVDF film-based capacitive biosensors can be properly applied to glucose sensing and provide opportunities for the low-cost fabrication of glucose-based biosensors based on PVDF materials.

Fe Doped Mno₂ Nanostructured Thin Films: Synthesis and Characterization for Biosensor applications

“…Figure7 indicates the correlation of sensitivity between pure MnO2 and 4 at% Fe: MnO2 by changing the glucose concentration. It is found that both of the sensitivities change exponentially which satisfy the results reported by Hartono et al [19] and Kondo et al [20]. The sensor requires an elevated operating temperature to enhance redox reactions to achieve the optimum conditions.…”

Fabrication of capacitive yarn torsion sensors based on an electrospinning coating method

“…Poly(vinylidene fluoride) (PVDF) is a promising organic material due to its excellent thermal and chemical stabilities, durability and biocompatibility . It is known that the outstanding crystal structure derived from the conformation of fluorine and hydrogen, which are periodically bonded to the PVDF chain, confers various capacitive responses, enabling electronic applications of PVDF in devices such as actuators, power generators and sensors …”

“…1,2 It is known that the outstanding crystal structure derived from the conformation of fluorine and hydrogen, which are periodically bonded to the PVDF chain, confers various capacitive responses, 3,4 enabling electronic applications of PVDF in devices such as actuators, [5][6][7] power generators [8][9][10] and sensors. [11][12][13][14] Capacitive sensors offer numerous advantages in terms of their capability to provide selectivity and higher sensitivity compared to resistive sensors that inherently require higher electric conductivity. 15 The basic structure of capacitive sensors comprises a dielectric material and two electrodes at top and bottom, which sensitively respond to external stimuli based on the dielectric constant, the distance between the two electrodes and the area of the interfaces between dielectric material and electrode.…”

Electrostatic Read Out for Label-Free Assays Based on Kelvin Force Principle

“…Regarding sensitivity, the serum fasting-level of ribose in human is 100 μM [11] Hence, the concentration of 6 mM we used here is still much higher. In contrast, reported values for experimental biosensors for glucose detection can range from 0.013 to 5.85 M [12] while others can reach as low as 300 nM as a detection limit [13]. Despite our system has not yet been tested under low concentrations of sugar, it is well known that KFM is capable of detecting changes in surface potential in the order of a few mV [14], which is a remarkable feature to be exploited in label-free detection technology.…”