Glucose tolerance factor stimulates 3-0-methylglucose transport into isolated rat adipocytes

Tokuda, Masakuni; Kashiwagi, Atsunori; Wakamiya, Eiji; Oguni, Tatsuya; Mino, Makoto; Kagamiyama, Hiroyuki

doi:10.1016/0006-291x(87)91443-4

Cited by 16 publications

(11 citation statements)

References 11 publications

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“…He suggested that insulin exerts its influence upon glucose transport by increasing the number of avail able carriers, and not by a change of affinity of the cariers to the substrate. Tokuda et al [9] examined glucose transport by adipocytes, prepared from normal orCr-depleted rats, and found a significant effect of GTF on glucose transport in the absence of insulin for both cases. He did not find any additional effect when he added insulin with GTF.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, GTF stimulates glucose utilization [6,7] and uptake by adipocytes in the presence [10], or even absence, of insulin [9]. The above Find ings, showing a GTF effect as an insulin like compound, on both yeast and animal cells, raised the possibility that insulin might affect glucose metabolism in the yeast.…”

Section: Introductionmentioning

confidence: 99%

“…Significant ef fects of GTF on glucose metabolism have been found in vivo and in vitro. These include decreased blood glucose levels in diabetic ani mals [1][2][3] and humans [4,5], stimulation of CO2 production from glucose in adipocytes [6][7][8] and enhancement of glucose transport into these cells [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Mirsky

Berdicevsky

1994

Neurosignals

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Addition of mammalian insulin or glucose tolerance factor (GTF), to Saccharomyces cerevisiae ATCC 7752, increased 2-deoxyglucose uptake by yeast cells. The enhancement was dose-dependent, and also dependent on preincubation of the cells with glucose, prior to the addition of the hormone. The effect of insulin on glucose uptake by the yeast was very similar to the effect of GTF on these cells. Insulin and GTF increased also the growth of yeast cells, while denatured insulin or glucagon failed to act. Our findings support the view that both insulin and GTF might regulate carbohydrate metabolism in microorganisms.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Mirsky

Berdicevsky

1994

Neurosignals

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“…Tokuda et al, [62] examined GTF obtained from yeast extract powder on glucose uptake in adipocytes. They found a stimulation of glucose uptake (5.6 times greater than the basal level) in the absence of insulin.…”

Section: Grant and Mcmullenmentioning

confidence: 99%

Glucose Tolerance Factor – Insulin Mimetic and Potentiating Agent – A Source for a Novel Anti Diabetic Medication

Mirsky¹

2012

Glucose Tolerance

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“…Uptake was initiated on addition of trace D-[U-14 C]glucose to a concentration of 0.38 mM. Thereafter, at both 1 and 21 min, 0.1-ml aliquots of well-swirled cell suspensions were removed and placed over 0.25 ml of silicone oil in 0.4-ml microcentrifuge polypropylene tubes and centrifuged, and the radioactivity in the floating cell cake was measured (22)(23)(24)(25). The difference in radioactivity between the two time points was used to calculate the clearance (fl/s per cell).…”

Section: Metabolic Incubationsmentioning

confidence: 99%

Diminished in Situ Glucose-6-Phosphate Flux in Permeabilized Adipocytes From Streptozocin-Induced Diabetic Rats

1991

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With metabolically active, saponin-permeabilized adipocytes, in situ pathway metabolism, which was distal to glucose transport, was examined in acute streptozocin-induced diabetic (STZ-D) rats. Metabolic reactions were initiated with selectively radiolabeled glucose-6-phosphate (G6P), an otherwise inert substrate with intact cells. Thus, the membrane pores permitted a direct comparison of cellular flux between control and STZ-D adipocytes at identical initial substrate concentrations. Three metabolic pathways were studied: 1) proximal glycolysis through the triosephosphates ([3-3H]G6P to 3H2O), 2) glycolysis-Krebs ([6-14C]G6P) oxidation, and 3) lipogenesis ([6-14C]G6P incorporation into triglyceride). The extent of membrane porosity was assessed by both propidium iodide staining and lactate dehydrogenase leakage to assure that porosity was comparable between the cell groups. Porous adipocytes from STZ-D rats had markedly attenuated rates of G6P metabolism compared with controls. At enzyme-saturating concentrations of G6P (4 mM), this deficit ranged from 44% for glycolysis-Krebs oxidation to 88% for lipogenesis. The reduction in glycolysis-Krebs oxidation was also evident between 0.5 and 6 mM G6P. These porous-cell data were compared with parallel studies of glucose metabolism and clearance in intact adipocytes. Finally, several glycolytic enzymes and acetyl-CoA carboxylase were measured in cell-free (sonicated) extracts with traditional in vitro methods under Vmax conditions. Overall, the in situ porous-cell flux measurements uncovered larger deficits in posttransport cellular metabolism than were apparent in the cell-free, in vitro assays. We conclude that, in actively metabolizing porous rat adipocytes, there exists a striking and unequivocal transport-independent defect in intermediary metabolism after acute STZ-D.

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Glucose tolerance factor stimulates 3-0-methylglucose transport into isolated rat adipocytes

Cited by 16 publications

References 11 publications

Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Effects of Insulin and Glucose Tolerance Factor on Glucose Uptake by Yeast Cells

Glucose Tolerance Factor – Insulin Mimetic and Potentiating Agent – A Source for a Novel Anti Diabetic Medication

Diminished in Situ Glucose-6-Phosphate Flux in Permeabilized Adipocytes From Streptozocin-Induced Diabetic Rats

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