Glucose utilization in sheep

Annison, E. F.; White, R. R.

doi:10.1042/bj0800162

Cited by 141 publications

(74 citation statements)

References 21 publications

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“…Glucose and lactate were estimated by enzymic methods (Barker and Britton 1957;Huggett and Nixon 1957). The 14C02 produced by the spermatozoa from 14C-Iabelled substrates was assayed as barium carbonate by the procedure of Annison and White (1961). After determining the initial and final levels of lactate and glucose in the flasks, the total amount of glucose utilized, the amount of lactate accumulated, and the amount of lactate formed could be calculated.…”

Section: (D) Analytical Methodsmentioning

confidence: 99%

The Fallopian Tube of the Sheep Iv. The Metabolism of Ram Spermatozoa in the Presence of Fluid from the Fallopian Tube

Restall¹,

Wales²

1966

Aust. Jnl. Of Bio. Sci.

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SummaryUsing isotopically labelled substrates, the metabolism of ram spermatozoa was examined in the presence of fluid from the fallopian tubes of normal ewes and spayed ewes receiving oestrogen and progesterone in factorial combination. The oxygen uptake of spermatozoa in tubal fluid was variable but was generally less than that of spermatozoa incubated in a saline diluent containing glucose (control). Due to the presence of lactate in the tubal fluids, the oxidation of added glucose by spermatozoa was consistently depressed when compared with the saline controls. On the other hand glucose utilization and lactate accumulation by spermatozoa were stimulated in the presence of tubal fluids in all experiments, the response being generally twice that of the controls. In addition, similar effects were found in fluids collected during two consecutive oestrous cycles.The hormonal treatments had little effect on the metabolism of spermatozoa. There were no differences in response to fluids collected during different stages of the oestrous cycle.

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Section: (D) Analytical Methodsmentioning

confidence: 99%

The Fallopian Tube of the Sheep Iv. The Metabolism of Ram Spermatozoa in the Presence of Fluid from the Fallopian Tube

Restall¹,

Wales²

1966

Aust. Jnl. Of Bio. Sci.

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“…Blood CO2 was isolated as BaCO3 (Annison & White, 1961) and assayed for radioactivity as described below.…”

Section: Chemical Methodsmentioning

confidence: 99%

Mammary and whole animal metabolism of glucose and fatty acids in fasting lactating goats

Annison¹,

Linzell²,

West³

1968

The Journal of Physiology

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SUMMARY1. Measurements were made of milk yield, mammary blood flow and mammary arteriovenous differences during the measurement of substrate entry rate by the isotope dilution method using [U-14C] acids at 094-6-8 mg/min/kg and 6-9 % of total CO2 was derived from each. The udder took up 3-0-5*7 mg/min/kg of tissue and 4-8 % of mammary CO2 was derived from each acid. In the udder 8 and 5-5 % of stearate and oleate were oxidized and 25 % of palmitate. Mammary uptake of stearate was 31-5 % of the total entry rate, palmitate 1 %, and oleate 7-5 %. Only long chain milk fatty acids were labelled. 6. During fasting the mammary R.Q. was 0-85 + 0 045 compared with a value in fed animals of 1-24 + 0-02, when the udder is synthesizing fatty acids from acetate. The total mammary uptake of lipid precursors was only 74 % of the rate of milk fat secretion and there was an 18 % shrinkage in empty udder volume, suggesting the use of endogenous mammary tissue substrates.

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“…Adult sheep have an entry rate of about 120 gjday (Annison and White 1961), and the contribution of both kidneys is not more than about 2 gjday, i.e. less than 2 %.…”

Section: Discussionmentioning

confidence: 99%

Kidney Function and Net Glucose Production in Normal and Acidotic Sheep

McIntosh¹,

Filsell²,

Jarrett³

1973

Aust. Jnl. Of Bio. Sci.

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Net glucose production and renal function were assessed simultaneously in normal sheep which were subsequently made acidotic by ammonium chloride administration. Arteriovenous differences in glucose concentration were measured, together with p-aminohippuric acid and inulin clearances in one kidney. In normal sheep there was a small net output of glucose by the kidney [20 Ilg min-1 (kg body weight)-l] which was increased threefold during acidosis. The increase was associated with an elevated venous-arterial difference in glucose concentration and a fall in renal blood flow, while glomerular filtration rate did not change. The increase in the proportion of blood being filtered indicates that there is a greater perfusion of the active zone of the cortex, which is the major site of glucose production in the kidney. This suggests that changed intrarenal events are associated with the physiological response of the kidney to altered acid-base status. The contribution by the kidneys to the overall glucose requirements in sheep, even during acidosis, is unlikely to be greater than 6%.

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Glucose utilization in sheep

Cited by 141 publications

References 21 publications

The Fallopian Tube of the Sheep Iv. The Metabolism of Ram Spermatozoa in the Presence of Fluid from the Fallopian Tube

The Fallopian Tube of the Sheep Iv. The Metabolism of Ram Spermatozoa in the Presence of Fluid from the Fallopian Tube

Mammary and whole animal metabolism of glucose and fatty acids in fasting lactating goats

Kidney Function and Net Glucose Production in Normal and Acidotic Sheep

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