2006
DOI: 10.1038/sj.jcbfm.9600400
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Glutamate is Preferred over Glutamine for Intermediary Metabolism in Cultured Cerebellar Neurons

Abstract: The glutamate-glutamine cycle is thought to be of paramount importance in the mature brain; however, its significance is likely to vary with regional differences in distance between astrocyte and synapse. The present study is aimed at evaluating the role of this cycle in cultures of cerebellar neurons, mainly consisting of glutamatergic granule cells. Cells were incubated in medium containing [U-13 C]glutamate or [U-13 C]glutamine in the presence and absence of unlabeled glutamine and glutamate, respectively. … Show more

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Cited by 45 publications
(61 citation statements)
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“…In contrast, many pathways entering or exiting glycolysis or the TCA cycle, such as those for lactate and glutamate metabolism, were clearly compartmentalized, with some reactions catalyzed by neurons and others catalyzed by astrocytes. These expression-based observations were confirmed by direct metabolic measurements, by exposing acutely isolated GLT1 ϩ astrocytes to [U-13 C]glucose and analyzing the labeled intermediates by mass spectrometry (MS) (Bak et al, 2006;McKenna et al, 2006;Olstad et al, 2007). TCA cycle intermediates and derivates, such as citrate, malate, and glutamate, contained 13 C label from [U-13 C]glucose, confirming that cortical astrocytes have a robust capacity for oxidative metabolism.…”
Section: Introductionmentioning
confidence: 62%
“…In contrast, many pathways entering or exiting glycolysis or the TCA cycle, such as those for lactate and glutamate metabolism, were clearly compartmentalized, with some reactions catalyzed by neurons and others catalyzed by astrocytes. These expression-based observations were confirmed by direct metabolic measurements, by exposing acutely isolated GLT1 ϩ astrocytes to [U-13 C]glucose and analyzing the labeled intermediates by mass spectrometry (MS) (Bak et al, 2006;McKenna et al, 2006;Olstad et al, 2007). TCA cycle intermediates and derivates, such as citrate, malate, and glutamate, contained 13 C label from [U-13 C]glucose, confirming that cortical astrocytes have a robust capacity for oxidative metabolism.…”
Section: Introductionmentioning
confidence: 62%
“…3A). A coupling of aspartate and glutamate metabolism has been demonstrated in previous studies [36].…”
Section: Hippocampal Formationmentioning
confidence: 71%
“…This is consistent with a more generalized exchange between lactate and pyruvate/alanine pools in undifferentiated cells than in differentiated ones. This compartmentalized model of cell metabolism has been discussed thoroughly in literature both in cell cultures [33,34] and in isolated perfused organs [35]. The pool of oxidizable pyruvate is more accurately defined by the pool of alanine, and an increase in alanine fractional enrichment in differentiated cells denotes a targeting of [U-13 C]glucose for oxidative metabolism in detriment of lactate production.…”
Section: Discussionmentioning
confidence: 99%