Glutamate measured by 6-s resolution brain microdialysis: capillary electrophoretic and laser-induced fluorescence detection application

Tucci, Sonia; Rada, Pedro; Sepúlveda, Milena; Hernández, Luis

doi:10.1016/s0378-4347(96)00488-4

Cited by 60 publications

(52 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…They obtained a limit of concentration detection at 10 À10 M level. The introduction of post-column detection in a sheat flow cuvette [14] or collinear geometry [26] lowered the limit of concentration detection to 10 À13 M. These improvements made single molecule detection the ultimate level of precision for CZE analysis [15,[27][28][29][30]. As a consequence, the CE high resolution power and sensitivity within the zeptomolar range (10 À21 M) made possible the successful use of CE-LIFD in endogenous neuroactive substances, DNA and illicit drug analysis [5,6,31].…”

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

“…But FITC requires longer times of derivatization than the other previously mentioned dyes. It is necessary to have at least 16-18 h of incubation to obtain stable and well detected FITC derivatives [29,30]. CE-LIFD analysis of FITC derivatives gives subattomolar determination of AA mixture, which represents few thousands of injected molecules [14,27,29,39,40].…”

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

“…Thus, CE with its very small sample volume requirements, high-resolution power, high sensitivity detection method is the alternative to HPLC in order to analyze microdialysates collected in very short times, at very low flow rate to increase the analyte concentration in the sample. In this way, it is possible to improve microdialysis temporal resolution [15,22,29,31] from 20-min sampling period to a few minutes or even seconds [30,50,51]. The on-line coupling of microdialysis with a continuous flow derivatization has been accomplished preserving the high temporal resolution of microdialysis and preventing losses of sample.…”

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

“…In the last decade, most of the determinations of classical neurotransmitters such as AA and monoamines, peptides and other neuroactive compounds have been done with CE-LIFD [29,30,37,51,117,118,120]. The use of CZE-LIFD in combination with brain microdialysis and CSF analysis has shed some light on central nervous system (CNS) function as well as its disorders.…”

Section: Ce-lifd Coupled To Microdialysis Studies Neuroactive Substamentioning

confidence: 99%

“…The combination of CZE high efficient separation and LIFD high sensitivity detection, in a fused silica capillary filled with alcaline carbonate buffer, is especially good for acidic AA such as glutamate (Glu) and aspartate (Asp), and for basic AA such as arginine (Arg) and lysine (Lys). The detection limit reaches the subattomol level [14,15,26,29,30,116]. The applicability of CE may be enhanced if the spectral analysis is incorporated.…”

Section: Ce-lifd Coupled To Microdialysis Studies Neuroactive Substamentioning

confidence: 99%

See 4 more Smart Citations

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

show abstract

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

Section: Ce Coupling To Microdialysismentioning

confidence: 99%

Section: Ce-lifd Coupled To Microdialysis Studies Neuroactive Substamentioning

confidence: 99%

Section: Ce-lifd Coupled To Microdialysis Studies Neuroactive Substamentioning

confidence: 99%

See 3 more Smart Citations

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

show abstract

In vivomonitoring of amine neurotransmitters using microdialysis with on-line capillary electrophoresis

2001

View full text Add to dashboard Cite

Microdialysis sampling was coupled via a flow-gated interface on-line to capillary electrophoresis with laser-induced fluorescence (LIF) detection for in vivo monitoring of neuroactive amino acids and amines. In the instrument, analytes are derivatized precolumn with o-phthaldehyde and beta-mercaptoethanol to form fluorescent isoindole products. The instrument was improved over previous designs by incorporating a sheath-flow cuvette for reduced background in LIF detection which improved sensitivity by 15-fold. The methodology was improved by utilizing a voltage ramped injection which allowed generation of 500000 theoretical plates with 20 s separations. Resolution of the isoindole derivatives was further improved by addition of hydroxypropyl-modified beta-cyclodextrin to the electrophoresis buffer. The new instrumentation and methods allow resolution and detection of glutamate, gamma-aminobutyric acid, glycine, aspartate, serine, taurine, glutamine and dopamine (if levels are elevated) collected from in vivo sampling probes every 20 s. The technique is suited to continuous monitoring for dynamic measurements of these compounds in vivo.

show abstract

Microdialysis Sampling as a Sample Preparation Method

Nandi

Lunte

2010

Handbook of Sample Preparation

View full text Add to dashboard Cite

Glutamate measured by 6-s resolution brain microdialysis: capillary electrophoretic and laser-induced fluorescence detection application

Cited by 60 publications

References 25 publications

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

In vivomonitoring of amine neurotransmitters using microdialysis with on-line capillary electrophoresis

Microdialysis Sampling as a Sample Preparation Method

Contact Info

Product

Resources

About