Glutamate receptor GluA1 subunit is implicated in capsaicin induced modulation of amygdala LTP but not LTD

Gebhardt, Christine; Albrecht, Doris

doi:10.1101/lm.045948.117

Cited by 3 publications

(19 citation statements)

References 51 publications

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“…We have recently extended these findings by showing that transcriptional changes can also be detected after a long-term memory can no longer be recalled (Perez, Patel, Rivota, Calin-jageman, & Calin-Jageman, 2018). Specifically, we used microarray and qPCR to identify changes in gene expression that accompany forgetting of a long-term sensitization memory in Aplysia californica .…”

Section: Introductionmentioning

confidence: 78%

“…We have previously shown that savings memory is apparent 7 days after sensitization training, a time point at which recall has become impossible for most animals (Perez et al, 2018). Here we sought to determine if savings memory persists even longer, testing 14 days after training (protocol in Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

“…This consisted of two moderate shocks (60Hz biphasic DC pulse for 2s at 20ma of constant current) applied to the midline of the tail with a 15-minute rest between the shocks. This protocol is strong enough to produce short-term sensitization in naïve animals, but not sufficient to produce long-term sensitization (Perez et al, 2018; Philips et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

“…For the savings experiment, we set a goal of collecting 21 animals, as this had been sufficient for our previous study of savings (Perez et al, 2018). For qPCR, we have previously found that 8–10 samples is sufficient to regularly detect the large changes in gene expression that can accompany long-term sensitization training (Conte et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…In the protocol we use, sensitization is forgotten within ~ 7 days, indicated by a progressive decline in T-SWR duration back to baseline levels (Conte et al, 2017). This apparent forgetting, however, belies a persistent savings memory, as a brief re-training can produce a long-lasting re-expression of the sensitization memory (Perez et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional changes before and after forgetting of a long-term sensitization memory in Aplysia californica

Patel

Perez

Farrell

et al. 2018

Neurobiology of Learning and Memory

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Most long-term memories are forgotten, becoming progressively less likely to be recalled. Still, some memory fragments may persist, as savings memory (easier relearning) can be detected long after recall has become impossible. What happens to a memory trace during forgetting that makes it inaccessible for recall and yet still effective to spark easier re-learning? We are addressing this question by tracking the transcriptional changes that accompany learning and then forgetting of a long-term sensitization memory in the tail-elicited siphon withdrawal reflex of Aplysia californica. First, we tracked savings memory. We found that even though recall of sensitization fades completely within 1 week of training, savings memory is still detectable at 2 weeks post training. Next, we tracked the time-course of regulation of 11 transcripts we previously identified as potentially being regulated after recall has become impossible. Remarkably, 3 transcripts still show strong regulation 2 weeks after training and an additional 4 are regulated for at least 1 week. These long-lasting changes in gene expression always begin early in the memory process, within 1 day of training. We present a synthesis of our results tracking gene expression changes accompanying sensitization and provide a testable model of how sensitization memory is forgotten.

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Section: Introductionmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptional changes before and after forgetting of a long-term sensitization memory in Aplysia californica

Patel

Perez

Farrell

et al. 2018

Neurobiology of Learning and Memory

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Registered Report: Transcriptional Analysis of Savings Memory Suggests Forgetting is Due to Retrieval Failure

Rosiles

Nguyen

Duron

et al. 2020

eNeuro

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There is fundamental debate about the nature of forgetting: some have argued that it represents the decay of the memory trace, others that the memory trace persists but becomes inaccessible because of retrieval failure. These different accounts of forgetting lead to different predictions about savings memory, the rapid re-learning of seemingly forgotten information. If forgetting is because of decay, then savings requires re-encoding and should thus involve the same mechanisms as initial learning. If forgetting is because of retrieval failure, then savings should be mechanistically distinct from encoding. In this registered report, we conducted a preregistered and rigorous test between these accounts of forgetting. Specifically, we used microarray to characterize the transcriptional correlates of a new memory (1 d after training), a forgotten memory (8 d after training), and a savings memory (8 d after training but with a reminder on day 7 to evoke a long-term savings memory) for sensitization in Aplysia californica ( n = 8 samples/group). We found that the reactivation of sensitization during savings does not involve a substantial transcriptional response. Thus, savings is transcriptionally distinct relative to a newer (1-d-old) memory, with no coregulated transcripts, negligible similarity in regulation-ranked ordering of transcripts, and a negligible correlation in training-induced changes in gene expression ( r = 0.04 95% confidence interval (CI) [–0.12, 0.20]). Overall, our results suggest that forgetting of sensitization memory represents retrieval failure.

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High Morphine Use Disorder Susceptibility Is Predicted by Impaired Learning Ability in Mice

et al. 2022

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An obvious reason for substance uses disorders (SUDs) is drug craving and seeking behavior induced by conditioned context, which is an abnormal solid context memory. The relationship between susceptibility to SUD and learning ability remains unclear in humans and animal models. In this study, we found that susceptibility to morphine use disorder (MUD) was negatively correlated with learning ability in conditioned place preference (CPP) in C57 mice. By using behavioral tests, we identified the FVB mouse as learning impaired. In addition, we discovered that learning-relevant proteins, such as the glutamate receptor subunits GluA1, NR1, and NR2A, were decreased in FVB mice. Finally, we assessed the context learning ability of FVB mice using the CPP test and priming. We found that FVB mice had lower learning performance with respect to normal memory but higher performance of morphine-reinstatement memory. Compared to C57 mice, FVB mice are highly sensitive to MUDs. Our results suggest that SUD susceptibility is predicted by impaired learning ability in mice; therefore, learning ability can play a simple and practical role in identifying high-risk SUD groups.

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Glutamate receptor GluA1 subunit is implicated in capsaicin induced modulation of amygdala LTP but not LTD

Cited by 3 publications

References 51 publications

Transcriptional changes before and after forgetting of a long-term sensitization memory in Aplysia californica

Transcriptional changes before and after forgetting of a long-term sensitization memory in Aplysia californica

Registered Report: Transcriptional Analysis of Savings Memory Suggests Forgetting is Due to Retrieval Failure

High Morphine Use Disorder Susceptibility Is Predicted by Impaired Learning Ability in Mice

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