Glutamine: A Suitable Nitrogen Source for Enhanced Shoot Multiplication in Cucumis sativus L.

Ayyappan, Vasudevan; Selvaraj, N.; Ganapathi, A.; Kasthurirengan, S.; Anbazhagan, V. Ramesh; Manickavasagam, Markandan

doi:10.1023/b:biop.0000024288.82679.50

Cited by 51 publications

(43 citation statements)

References 13 publications

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“…As easily accessible source of reduced nitrogen, it is generally more favorable than ammonium, to morphogenesis as reported for Cucumis sativus L. In the course of work done with the in vitro multiplication of Arachis hypogea L. from cotyledon explants, the presence of L-glutamine also improved and maintained high multiplication and growth rates. During transamination, glutamine transfers nitrogen to other carbohydrated substrates ( -keto acids) and the new amino acids immediately contribute to the biosynthesis of proteins [12,13]. The glutamine and glutamate byproduct are the primary sources of reduced nitrogen in the cellular metabolism.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l -1 citric acid, 12.2 mg.l -1 adenine sulfate, 15 mg.l -1 L-arginine, 2.46 M IBA (indole-3-butyric acid), 30 g.l -1 sucrose and 7 g.l -1 of agar, and enriched with different balances of BA (benzyladenine) and L-glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 M BA and 25 mg.l -1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in an MS medium supplemented with 2.21 M BA, 5.70 M IAA (indole-3-acetic acid) and 15 mg.l -1 L-arginine. These shoots were isolated and then rooted in MS containing 2.46 M of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable.

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Section: Discussionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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“…Numerous reports specify that reduced nitrogen forms, particularly amides and amino acids, can improve cell proliferation as well as regeneration in specific genotypes (Vasudevan et al, 2004).…”

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confidence: 99%

L-arginine impact on cherry rootstock rooting and biochemical characteristics in tissue culture

Sarropoulou¹,

Dimassi-Theriou²,

Therios³

2014

Turk J Agric For

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In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. In the CAB-6P rootstock, the best root number and root length results were obtained with 2 mg L -1 IBA plus 0.5 mg L -1 L-arginine and 1 mg L -1 IBA plus 1 mg L -1 L-arginine, respectively. The rooting percentage was highest (100%) with 2 mg L -1 IBA alone or combined with 1 mg L -1 L-arginine. In the Gisela 6 explants, 2 mg L -1 IBA without L-arginine significantly enhanced root number (9.27) and root fresh and dry weight, as well as rooting percentage (100%). Root length was longest (38 mm) in the combination of the lowest IBA (0.5 mg L -1 ) and highest L-arginine (2 mg L -1 ) concentrations. Thus, L-arginine promotes the positive effect of IBA on rooting with regard to both root number and root length in both cherry rootstocks. In the CAB-6P explants, L-arginine combined with IBA had an inhibitory effect on leaf chlorophyll content, whereas in the Gisela 6 rootstock it had absolutely no effect. In the CAB-6P rootstock, depleted levels of proline in roots were observed, showing the osmoregulation and osmotic adjustment mechanisms. The carbohydrate concentration in leaves was greatest with 0.5 mg L -1 IBA plus 0.5 mg L -1 L-arginine, and in roots with 2 mg L -1 IBA plus 0.5 mg L -1 L-arginine. In the Gisela 6 explants, IBA (1 and 2 mg L -1 ) applied both alone and along with L-arginine, as well as the combination of the lowest IBA (0.5 mg L -1 ) and the highest L-arginine (2 mg L -1 ) concentrations, considerably increased leaf carbohydrate concentration compared to the control. In roots, carbohydrate concentration was maximum with 2 mg L -1 IBA plus 0.5 mg L -1 L-arginine. The elevated levels of proline in roots with 1 mg L -1 IBA plus 0.5 mg L -1 L-arginine indicate stressful conditions.

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“…Thomas and Sreejesh (2004) have reported that the addition of 0.2 μM NAA with 4 μM 6-benzylaminopurine (BAP) was beneficial for adventitious shoot regeneration in the cotyledon-derived callus of ash gourd. When compared with cucumber (Vasudevan et al, 2004;Vasudevan et al, 2007b), watermelon (Sultana and Bari, 2003;Sultana et al, 2004), melon (Curuk et al, 2002;Muruganantham et al, 2002) and squash (Ananthakrishnan et al, 2003;Kathiravan et al, 2006), chieh-qua also needed a high concentration of 6-BA for shoot induction. Such a high concentration may influence shoot proliferation as well as elongation, and could be one of the reasons for the difficulty of regeneration of chieh-qua.…”

Section: Discussionmentioning

confidence: 99%