Glutamine attenuates tumor necrosis factor-α release and enhances heat shock protein 72 in human peripheral blood mononuclear cells

Wischmeyer, Paul E.; Riehm, Jacob J.; Singleton, Kristen D.; Ren, Hao; Musch, Mark W.; Kahana, Madelyn; Chang, Eugene B.

doi:10.1016/s0899-9007(02)00839-0

Cited by 126 publications

(85 citation statements)

References 21 publications

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“…In contrast, glutamine in a concentration equivalent to that found in normal plasma markedly increases HSP72 expression in mononuclear cells following LPS treatment (Wischmeyer et al, 2003). Naka et al (1996) examined the effect of an intravenous glutamine dipeptide administration on septic rats and found that mortality was significantly lower in the glutamine-total parenteral nutrition group than in animals receiving conventional diet.…”

Section: Cell Defense and Repairmentioning

confidence: 99%

“…This topic has been reviewed by Newsholme (2001). Expression and production of TNF-a by cultured mononuclear cells stimulated with lipopolyssacharide (LPS) can be suppressed by glutamine (2-10 mM) (Pithon-Curi et al, 2002;Wischmeyer et al, 2003). Opposite effects were found for the synthesis and secretion of IL-1b and IL-6 in LPS-stimulated rat peritoneal macrophages.…”

Section: Modulation Of Immune Functionmentioning

confidence: 99%

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Molecular mechanisms of glutamine action

Curi

Lagranha

Doi

et al. 2005

Journal Cellular Physiology

416

300

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Glutamine is the most abundant free amino acid in the body and is known to play a regulatory role in several cell specific processes including metabolism (e.g., oxidative fuel, gluconeogenic precursor, and lipogenic precursor), cell integrity (apoptosis, cell proliferation), protein synthesis, and degradation, contractile protein mass, redox potential, respiratory burst, insulin resistance, insulin secretion, and extracellular matrix (ECM) synthesis. Glutamine has been shown to regulate the expression of many genes related to metabolism, signal transduction, cell defense and repair, and to activate intracellular signaling pathways. Thus, the function of glutamine goes beyond that of a simple metabolic fuel or protein precursor as previously assumed. In this review, we have attempted to identify some of the common mechanisms underlying the regulation of glutamine dependent cellular functions.

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Section: Cell Defense and Repairmentioning

confidence: 99%

Section: Modulation Of Immune Functionmentioning

confidence: 99%

Molecular mechanisms of glutamine action

Curi

Lagranha

Doi

et al. 2005

Journal Cellular Physiology

416

300

View full text Add to dashboard Cite

show abstract

“…The results of recent studies show the regulative impact of glutamine on the inflammatory response in severe infections and indicate that it is necessary to administer high doses (e.g. in parenteral administration 0.35 g/kg × day) to obtain a better therapeutic effect [49,55,56].…”

Section: Glutaminementioning

confidence: 99%

Alterations in innate antibacterial response after immunomodulatory nutrition

Słotwiński¹,

Słotwińska²,

Kędziora³

et al. 2012

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Septic infections in malnourished surgical patients show the highest morbidity and mortality rate. The attempt to correct postoperative immune and nutritional disorders by introducing immune-enhancing nutrition (immunonutrition) is a promising way of improving outcome, but as yet little is known about the mechanisms of correcting an extensive postoperative inflammatory response (systemic inflammatory response syndrome [SIRS]) to a massive infection using this type of nutrition. A significant role in the innate antibacterial and inflammatory response is played by Toll-like receptors (TLRs) that recognize pathogen-associated molecular patterns (PAMPs). The regulatory impact of immunonutrition on TLR expression in surgical septic patients seems to be a new research direction. In this paper special emphasis was put on clinical trials and the research results for the TLR-dependent immune response and anti-bacterial/anti-inflammatory response applying immu nomo dulatory nutrition with increased concentrations of glutamine and unsaturated fatty acids. StreszczenieZakażenia septyczne w grupie niedożywionych pacjentów chirurgicznych obarczone są najwyższym wskaźnikiem chorobowości i śmiertelności. Korekcja pooperacyjnych zaburzeń odporności i niedożywienia poprzez żywienie immunomodulujące jest obiecującą metodą uzyskania lepszych wyników leczenia, ale nadal niewiele wiadomo o mechanizmach regulacji zwiększonej pooperacyjnej odpowiedzi zapalnej (systemic inflammatory response syndrome -SIRS) na masywne zakażenie za pomocą tego typu terapii. Istotne znaczenie w regulacji wrodzonej odpowiedzi przeciwbakteryjnej i przeciwzapalnej mają receptory TLRs (Toll-like receptors), rozpoznające związane z patogenami wzory molekularne (pathogen-associated molecular patterns -PAMPs). Ocena regulacyjnego wpływu żywienia immunomodulacyjnego na ekspresję receptorów TLR u septycznych pacjentów chirurgicznych jest nowym kierunkiem badań naukowych. W przedstawionej pracy szczególną uwagę zwrócono na wyniki badań klinicznych i eksperymentalnych dotyczące regulacji zależnej od TLRs odpowiedzi przeciwbakteryjnej/przeciwzapalnej za pomocą żywienia immunomodulacyjnego, zawierającego zwiększone stężenia glutaminy i nienasyconych kwasów tłuszczowych.

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“…Therefore, intracellularly activated HSP70s are cytoprotective and antiinflammatory by avoiding protein denaturation and excessive NF-B activation which may be damaging to the cells. It is strikingly noteworthy that L-glutamine attenuates TNF- release and enhances HSP72 expression in human peripheral blood mononuclear cells (Wischmeyer et al, 2003). In fact, L-glutamine induces HSP70 expression via O-glycosylation and phosphorylation of HSF-1 and Sp1 (Singleton, K.D.…”

Section: Intracellular Hsp70mentioning

confidence: 99%