Glutathione and related enzyme activity in human lung cancer cell lines

Carmichael, James; Mitchell, James B.; Friedman, Norman R.; Gazdar, Adi F.; Russo, Angelo

doi:10.1038/bjc.1988.236

Cited by 63 publications

(30 citation statements)

References 31 publications

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“…A549 cells possess enzymes necessary for metabolic activation (41) and have a high level of glutathione (42). We believe that this was crucial for lower LTN % in A549 than in HepG2 cells.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Tryptophol Genotoxicity in Four Cell Lines In Vitro: A Pilot Study with Alkaline Comet Assay

Kosalec¹,

Ramić²,

Jelić³

et al. 2011

Archives of Industrial Hygiene and Toxicology

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Tryptophol is an aromatic alcohol and secondary metabolite of the opportunistic fungus Candida albicans. Although its toxicity profi le at cell level has been poorly investigated, recent data point to cytotoxic, cytostatic, and genotoxic effects in lymphocytes and the induction of apoptosis in leukaemic blood monocytes. In this pilot study we evaluated the genotoxicity of tryptophol in vitro on four permanent cell lines of animal and human origin: ovary cells, alveolar epithelium, liver cells, and blood monocytes using the alkaline comet assay. We selected cells that might be principal targets of tryptophol and other low-molecular geno(toxins) secreted by Candida albicans during host invasion. Our results suggest that tryptophol applied in vitro at 2 mmol L -1 for 24 h damages DNA in HepG2, A549 and THP-1 cells, obviously due to bioactivation and/or decomposition of the parent compound, which results in the formation of more genotoxic compound(s) and production of reactive species that additionally damage DNA. On the other hand, notably lower levels of primary DNA damage were recorded in CHO cells, which lack metabolic activity. Future studies with tryptophol should look further into mechanisms involved in its toxic action and should focus on other cell types prone to infection with Candida spp. such as vaginal epithelial cells or keratinocytes of human origin.

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“…A549 cells possess enzymes necessary for metabolic activation (41) and have a high level of glutathione (42). We believe that this was crucial for lower LTN % in A549 than in HepG2 cells.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Tryptophol Genotoxicity in Four Cell Lines In Vitro: A Pilot Study with Alkaline Comet Assay

Kosalec¹,

Ramić²,

Jelić³

et al. 2011

Archives of Industrial Hygiene and Toxicology

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show abstract

“…Manipulation of cellular glutathione levels is known to influence the sensitivity of cells to UV and ionizing radiation (41) and thus represents a potential cytoprotective pathway. However, no consistent differences have been observed in the cellular content of glutathione or glutathione-metabolizing enzyme activities in classic and variant SCLC (42). A wealth of information has recently highlighted the p53 protein as an important modulator of (5) where loss of functional p53 may increase the threshold of tumor cells to entry into apoptosis.…”

Section: Discussionmentioning

confidence: 99%

c-Jun NH2-terminal Kinase Regulation of the Apoptotic Response of Small Cell Lung Cancer Cells to Ultraviolet Radiation

Butterfield

Storey

Maas

et al. 1997

Journal of Biological Chemistry

143

102

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“…Low amounts of the MDR1 gene product P-glycoprotein (P-gp), known to confer intrinsic MDR in various human malignancies (review: Bellamy and Dalton, 1994), have been detected in unselected NSCLC cell lines (review: Doyle, 1993). Reduced levels of topoisomerase II (Giaccone et al, 1992) and an increased amount of glutathione-S-transferases (Bai et al, 1996;Carmichael et al, 1988) have been observed in association with chemoresistance. However, all such drug resistance mechanisms could not explain entirely the observed MDR phenotype in NSCLC (Doyle, 1993).…”

mentioning

confidence: 99%

Expression of the multidrug resistance-associated protein (MRP) and chemoresistance of human non-small-cell lung cancer cells

Berger¹,

Elbling²,

Hauptmann³

et al. 1997

Int. J. Cancer

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Glutathione and related enzyme activity in human lung cancer cell lines

Cited by 63 publications

References 31 publications

Assessment of Tryptophol Genotoxicity in Four Cell Lines In Vitro: A Pilot Study with Alkaline Comet Assay

Assessment of Tryptophol Genotoxicity in Four Cell Lines In Vitro: A Pilot Study with Alkaline Comet Assay

c-Jun NH2-terminal Kinase Regulation of the Apoptotic Response of Small Cell Lung Cancer Cells to Ultraviolet Radiation

Expression of the multidrug resistance-associated protein (MRP) and chemoresistance of human non-small-cell lung cancer cells

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