Glutathione assisted preparation of gold nanoclusters using minimum amount of protein

Wu, Bo-Yi; Wang, Chia‐Wei; Chen, Po‐Cheng; Chang, Huan‐Tsung

doi:10.1016/j.snb.2016.09.071

Cited by 26 publications

(10 citation statements)

References 40 publications

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“…Wu et al use bovine serum albumin (BSA) and GSH to synthesize gold nanoclusters (BSA/GSH-AuNCs) with excitation and emissions at 330 nm and 650 nm, respectively [ 100 ]. In this approach, BSA and GSH serve primarily as a limitation and reducing agents, respectively.…”

Section: Biomacromolecule-conjugated Auncsmentioning

confidence: 99%

Biomedical Applications for Gold Nanoclusters: Recent Developments and Future Perspectives

et al. 2018

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Gold nanoclusters (AuNCs) have been extensively applied as a fluorescent probe for biomedical applications in imaging, detection, and therapy due to their unique chemical and physical properties. Fluorescent probes of AuNCs have exhibited high compatibility, superior photostablility, and excellent water solubility which resulted in remarkable biomedical applications for long-term imaging, high-sensitivity detection, and target-specific treatment. Recently, great efforts have been made in the developments of AuNCs as the fluorescent probes for various biomedical applications. In this review, we have collected fluorescent AuNCs prepared by different ligands, including small molecules, polymers, and biomacromolecules, and highlighted current achievements of AuNCs in biomedical applications for imaging, detection, and therapy. According to these advances, we further provided conclusions of present challenges and future perspectives of AuNCs for fundamental investigations and practical biomedical applications.

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Section: Biomacromolecule-conjugated Auncsmentioning

confidence: 99%

Biomedical Applications for Gold Nanoclusters: Recent Developments and Future Perspectives

et al. 2018

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“…Inorganic anions, such as cyanide (CN -), sulfide (S 2-), and (nitrite) NO 2-, retain the damaging influence on ecological systems as well as human health. 108,[132][133][134][135][136][137][138][139][140][141][142][143] Among them, acquaintance to high levels of CN-leads to the compensations of organs and skin via the suppression of oxygen transportation. 108,[132][133][134][135][136] Besides, hydrogen cyanide (HCN) is practical to be liberated from enzyme-catalyzed hydrolysis of cyanogenic glycosides that are commonly present in edible foods, like bamboo and cassava.…”

Section: Fluorescent Sensorsmentioning

confidence: 99%

Bio-Mediated Synthesis of Nanoparticles for Fluorescence Sensors

Vandarkuzhali

2021

Materials Research Foundations

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The nature deeds alike a hefty “bio-laboratory” embracing of plants, algae, fungi, yeast etc. which are poised of biomolecules. These indeed befalling biomolecules must stood notorious to play an active role in the establishment of nanoparticles through diverse shapes and sizes thus acting as a driving force intended for the scheming of greener, safe and environmentally benign protocols for the synthesis of nanoparticles. The contemporary chapter targets the proportional biogenic synthesis and mechanisms of nanoparticles using biomolecules. The practice of biomolecules not only diminishes the price of synthesis but also curtails the need of using hazardous chemicals and arouses ‘green synthesis’. It also emphases on aspects of binding of biomolecules to nanoparticles and certain of the applications of the biosynthesized nanoparticles as sensor for cations, anions and also biosensors.

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“…Thiol-containing small molecules, especially glutathione (GSH), are the most commonly used stabilizers due to the strong interactions between thiols and Au/Ag. − However, this kind of nanoclusters usually suffered from low quantum yield and poor stability against photoirradiation . Large proteins with abundant functional groups (e.g., amino, carboxyl, and sulfhydryl) could protect nanoclusters more efficiently and have been widely used for the preparation of nanoclusters. , Nanoclusters stabilized by mixed ligands such as GSH with protein or two kinds of proteins were reported to have better performance than nanoclusters capped with single ligand, such as stronger luminescence and stability. − …”

Section: Introductionmentioning

confidence: 99%

Au/Ag Bimetallic Nanoclusters Stabilized by Glutathione and Lysozyme for Ratiometric Sensing of H₂O₂ and Hydroxyl Radicals

Tang

Jin

et al. 2021

ACS Appl. Nano Mater.

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Metal nanoclusters, widely used as fluorescent probes in biosensing and bioimaging, still suffer from some barriers, such as low quantum yield, impurities, and poor sensitivity. Developing nanocluster-based fluorescent probes with good performance and a facile separation method for the synthesized nanoclusters to improve the sensing ability is of great importance. Herein, we reported a convenient approach to purify Au/Ag bimetallic nanoclusters stabilized by dual ligands of GSH and lysozyme (GSH-Lys@Ag-AuNCs) via isoelectric point precipitation, and the purified GSH-Lys@Ag-AuNCs exhibited an improved sensing sensitivity and linear range to the Fenton reagent (H2O2 + Fe2+). The fluorescence intensity ratio of F 440/F 640 was linear to the H2O2 concentration over the wide range of 0.2–500 μmol L–1 (R 2 = 0.97), with a low detection limit of 0.05 μmol L–1. Utilizing the catalytic oxidation reaction of the substrate under a specific oxidase by producing H2O2, the probe was further employed to glucose and uric acid assays with satisfactory results in human serum samples. Additionally, fluorescence confocal imaging experiments were performed to monitor the fluctuation of the •OH level in living cells, and the fluorescence intensity ratio of the blue channel to the red channel showed excellent ratiometric changes with the •OH contents.

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Glutathione assisted preparation of gold nanoclusters using minimum amount of protein

Cited by 26 publications

References 40 publications

Biomedical Applications for Gold Nanoclusters: Recent Developments and Future Perspectives

Biomedical Applications for Gold Nanoclusters: Recent Developments and Future Perspectives

Bio-Mediated Synthesis of Nanoparticles for Fluorescence Sensors

Au/Ag Bimetallic Nanoclusters Stabilized by Glutathione and Lysozyme for Ratiometric Sensing of H₂O₂ and Hydroxyl Radicals

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Glutathione assisted preparation of gold nanoclusters using minimum amount of protein

Cited by 26 publications

References 40 publications

Biomedical Applications for Gold Nanoclusters: Recent Developments and Future Perspectives

Biomedical Applications for Gold Nanoclusters: Recent Developments and Future Perspectives

Bio-Mediated Synthesis of Nanoparticles for Fluorescence Sensors

Au/Ag Bimetallic Nanoclusters Stabilized by Glutathione and Lysozyme for Ratiometric Sensing of H2O2 and Hydroxyl Radicals

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Au/Ag Bimetallic Nanoclusters Stabilized by Glutathione and Lysozyme for Ratiometric Sensing of H₂O₂ and Hydroxyl Radicals