1979
DOI: 10.1111/j.1432-1033.1979.tb13056.x
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Glutathione S‐Conjugate Formation from 1‐Chloro‐2,4‐dinitrobenzene and Biliary S‐Conjugate Excretion in the Perfused Rat Liver

Abstract: The isolated hemoglobin‐free perfused rat liver was used as a model to study the formation of a glutathione S‐conjugate and its disposition into the biliary as well as other extracellular (caval perfusate) compartments. 1‐Chloro‐2,4‐dinitrobenzene was chosen as substrate because it reacts with the glutathione‐S‐transferases at high activity to give rise to an S‐conjugate, S‐(2,4‐di‐nitrophenyl)‐glutathione, a product readily discernible from the parent compound. S‐(2,4‐Dinitrophenyl)‐glutathione formation rat… Show more

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Cited by 100 publications
(44 citation statements)
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“…As shown, the mBCI-GSH conjugation during the first 8 min initially follows a first-order kinetic reaction. However, the conjugation reaction will not reach a plateau due to the second-order kinetic reaction caused by a number of possible additional reactions [e.g., conjugation of mBCl to nonprotein thiols (21), new synthesis of GSH (3,17,34), feedback inhibition by mBC1-GSH on GST activity (9), slow leakage of the mBC1-GSII conjugate from cells (32)]. Curve-fitting regression analysis was performed using scans that follow the first-order kinetic reaction to minimize the confounding factors referenced above ( i c , where the correlation coefficient = 1 ).…”
Section: Analysis Of Gsh-mbci Fluorescencementioning
confidence: 99%
“…As shown, the mBCI-GSH conjugation during the first 8 min initially follows a first-order kinetic reaction. However, the conjugation reaction will not reach a plateau due to the second-order kinetic reaction caused by a number of possible additional reactions [e.g., conjugation of mBCl to nonprotein thiols (21), new synthesis of GSH (3,17,34), feedback inhibition by mBC1-GSH on GST activity (9), slow leakage of the mBC1-GSII conjugate from cells (32)]. Curve-fitting regression analysis was performed using scans that follow the first-order kinetic reaction to minimize the confounding factors referenced above ( i c , where the correlation coefficient = 1 ).…”
Section: Analysis Of Gsh-mbci Fluorescencementioning
confidence: 99%
“…The intracellular formation of glutathione Sconjugates is followed by their active extrusion from the cell [3-51. In liver, glutathione Sconjugates and glutathione disulfide (GSSG) are secreted preferentially into bile [4,6] by, what appears to be, a common transport system [7]. A similar transport system, involved in the extrusion * To whom correspondence should be addressed…”
Section: Introductionmentioning
confidence: 99%
“…In situ, CDNB is conjugated with GSH at a high rate to give DNPG [16]. Consequently, the interactions of this conjugate with the enzyme GSSG reductase were studied in detail.…”
Section: Discussionmentioning
confidence: 99%
“…In view of the high activity of these enzymes (120 U/g liver [16]), the concentration of CDNB is assumed to be low in situ. Since nitrated aromatic compounds are known as strong inhibitors of glutathione reductase [17] we tested the effect of CDNB on this enzyme in vitro and found a Ki value of 22 pM.…”
Section: Inhibition Of' Glutathione Reductase By I-chloro-24-dinitromentioning
confidence: 99%